Last updated on July 10, 2020
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松木 陽、理学博士、准教授
Yoh Matsuki, Ph.D., Associate Professor

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大阪大学 大阪大学蛋白質研究所
Institute for Protein Research
Osaka University

大阪大学 先導的学際研究機構 量子情報・量子生命研究センター
Quantum Information and Quantum Biology Division,
Institue for Open and Transdisciplinary Research Initiatives,
Osaka University

3-2 Yamadaoka, Suita, Osaka, 565-0871 Japan
+81 668 798 598 (office)
+81 668 798 599 (fax)
yoh@protein.osaka-u.ac.jp

RESEARCH INTERESTS

Methods and Instruments for High-Field Dynamic Nuclear Polarization (DNP)-Enhanced MAS NMR
-- 14.1 T and 16.4 T DNP MAS NMR Spectrometers
-- Closed-Cycle Helium MAS System for DNP at 20K -200 K
-- Dual 460 GHz-Gyrotron DNP System with Active Feedback Frequency Control
-- Novel Poralizing Agents based on Transition Metal Complexes

Methods and Software Distribution for Non-Uniform Sampling (NUS) Fast NMR Data Acquisition Techiniques
-- Spectroscopy by Integrating Frequency and Time domain information (SIFT) method
-- Application to R2 dispersion measurements of short-lived proteins

Structural Analysis of Amyloids and Membrane Proteins
-- Amyloid Fiblrils (Beta2m, a-Synuclein etc)
-- Sodium, Halo and Sensory Rhodopsins

WORK EXPERIENCES

2018-
Osaka University, Associate Professor
2009-2018
Osaka University, Assistant Professor
2006-2009
Massachusetts Institute of Technology (Massachusetts, USA), Visiting Scientist
in Prof. Robert G. Griffin Lab
2006-2009
Brandeis University (Massachusetts, USA), Post-Doctral Fellow
in Prof. Judith Herzfeld Lab
2003-2006
Japan Science and Technology Agency (JST), Post-Doctral Fellow
in Prof. Hideo Akutsu Lab

EDUCATION

-2003
Osaka University, Graduate School of Science
PhD, Chemistry
Dissertation: “Novel Methods for Structural Analysis by High-Resolution Solid-State NMR Spectroscopy utilizing the Double-Quantum Dipolar Interactions”
-2000
Chuo University, Graduate School of Science and Technology
BS, Applied Chemistry
Thesis: “NMR Study on the Interaction between a Platinum Complex [Pt(Phen)(Leu)] and Oligomeric DNA”
-1998
Chuo University, School of Science and Technology
BA, Applied Chemistry
Thesis: “Synthesis and Characterization of Cisplatin Analogue Complex Involving 1, 10-Phenanthroline and L-Leucine”

PATENTS

国内特許
藤原敏道, 松木 陽, 遠藤由宇生, 中村新治, 根本貴宏, “極低温条件でも抜けない試料管キャップ” 特願2018-081242, 2018
藤原敏道, 松木 陽, 中村新治, “循環方式冷却器を用いた動的核偏極固体高分解能NMR装置” 特願2015-016082, 2015

US patent
Olivier Ouari, Hakim Karoui, Francois LeMoigne, Paul Tordo, Robert G. Griffin, Yoh Matsuki and Thorsten Maly, “Dinitroxide-Type Biradical Compounds Optimized for Dynamic Nuclear Polarization (DNP),” U.S. Patent US9035054 B2, Issued on May 19th, 2015

International patent
2. T. Fujiwara, Yoh Matsuki and Shinji Nakamura, “DNP-NMR probe using cryogen circulation system,” International Patent Pending: G20150053 (US)(DE), 2015
1. Olivier Ouari, Hakim Karoui, Francois LeMoigne, Paul Tordo, Robert G. Griffin, Yoh Matsuki and Thorsten Maly, “Dinitroxide Biradical Compounds Optimized for Dynamic Nuclear Polarization (DNP),” International Patent Application: WO/2010/108995

INTERNATIONAL TALKS

Invited
25. International Society of Magnetic Resonance Conference (ISMAR), Osaka (August, 2021)
24. Weekly ssNMR/DNP Zoominar, Organized by: Kong Ooi Tan, Robert G. Griffin (MIT, USA), https://griffingroup.mit.edu/weekly-ssnmrdnp-zoominar (July, 2020)
23. 4th Ulm Meeting - Biophysics of Amyloid Formation, Ulm, Germany (February, 2020)
22. 2nd Indo-Japanese Bilateral Meeting on Magnetic Resonance, Hyderabad, India (December, 2019)
21. 14th International Symposium of the Institute Network for Biomedical Sciences, Osaka University (October, 2019)
20. EUROISMAR, Berlin, Germany (August, 2019)
19. TSRC Workshop, Emerging Methodologies for Paramagnetic NMR and Dynamic Nuclear Polarization in Biological and inorganic Materials, Telluride, CO, USA (August, 2019)
18. The 1st Asian-European International Exchange Symposium in Solid-State NMR, RIKEN-Yokohama (June, 2019)
17. Japan-Korea Bilateral Symposium on Multi-Scale Structural Life Science and Advanced Technologies, IPR, Osaka University (March, 2019)

16. The 3rd Joint Conference of Asia-Pacific EPR Society and the International EPR Society , The University of Queensland, Brisbane, Australia, September, 2018, “DNP-Enhanced MAS-NMR Spectroscopy at 16.4 T and 30 K, Instrumentation and Applications”
15. The 3rd International Conference on Spin Physics, Spin Chemistry, and Spin Technology, Novosibirsk, Russia, September 2018, “DNP-Enhanced MAS-NMR Spectroscopy at 16.4 T and 30 K, Instrumentation and Applications”
14. Big Mag @ University of California, Santa Barbara , University of California, Santa Barbara, USA, May 2018, “460 GHz-700 MHz DNP-NMR using Closed-Cycle Helium MAS and Dual Gyrotron System”
13. The 10th International Workshop on Strong Microwaves and Terahertz Waves, Nizhny Novgorod, Russia, July 2017, “Advanced Instrumentation for High-Field DNP for Sensitivity-Enhanced Biological Solid-State NMR”
12. The 5th Awaji lnternational Workshop on Electron Spin Science & Technology , Awaji Island, Japan, June 2017, “Advanced Instrumentation for High-Field DNP for Sensitivity-Enhanced Biological Solid-State NMR”
11. The 6th International Workshop on Far-Infrared Technologies, Univerisity of Fukui, Japan, March 2017, “Advanced Instrumentation for Sensitivity-Enhanced Biological Solid-State NMR”
10. The Second Trilateral Workshop for Frontier Protein Studies, Osaka Univerisity, Japan, June 2016, “Advanced Instrumentation for High-Field DNP for Sensitivity-Enhanced Biological Solid-State NMR”
9. International Chemical Congress of Pacific Basin Society (Pacifichem), Honolulu, HI, USA, December 2015, “Dynamic Nuclear Polarization at 16.4 T and 30 K using a Novel Closed-Cycle Helium-Cooling MAS NMR Probe System”
8. 19th International Workshop on Advanced Molecular Materials Science , Osaka City University, November 2015, “Advanced Instrumentations for DNP-Enhanced MAS NMR at High-Fields and Low Temperatures”
7. 57th Experimental NMR Conference (ENC), Asilomar, CA, USA, April 2015, “Closed-Cycle Helium-Cooling and -Spinning MAS NMR Probe System for DNP at 16.4 T”
6. APES-IES-SEST (International EPR Society Symposium), Nara, Japan, November 2014, “Dynamic Nuclear Polarization (DNP) for Biological Solid-State NMR at High Fields and Low Temperatures”
5. 7th Japanese-Russian International Workshop on Open-Shell Compounds and Molecular Spin Devises, Awaji, Japan, October 2013, “Dynamic Nuclear Polarization-Enhanced Solid-State NMR using High Field, Low Temperature and a Rigid Biradical”
4. 5th International Workshop on Far-Infrared Technologies, Fukui, Japan, March 2013, “Application of Continuously Frequency-Tunable 0.4-GHz Gyrotron for DNP-Enhanced Solid-State NMR”
3. 3rd International Meeting on DNP, Laussane, Switzerland, September 2011, “High-Field, High-Sensitivity Solid-State NMR by Dynamic Nuclear Polarization”
2. 48th Solid-State NMR and Material Science Forum, Tsukuba, Tsukuba, Japan October 2010, “High-Field, High-Sensitivity Solid-State NMR by Dynamic Nuclear Polarization”
1. Yokohama NMR Association for Structural Biology, Yokohama, Japan March 2010 “Spectroscopy by Integration of Frequency and Time Information”

Regular (Listed International mettings only)
1. Experimental NMR Conference (ENC), Miami, Florida, April 2012, "Helium-Cooling and -Spinning Dynamic Nuclear Polarization for Sensitivity Enhanced Solid-State NMR at 14T and 30K" Plenary Talk
2. The international symposium on nuclear magnetic resonance (ISNMR), Yokohama, Japan, November 2011, “High-Field, High-Sensitivity Solid-State NMR by Dynamic Nuclear Polarization”
3. Group Lecture after the Griffin-Herzfeld Reunion "Four Decades @ the Magic Angle", Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, January 2011 “High-Field, High-Sensitivity Solid-State NMR by Dynamic Nuclear Polarization”
4. Experimental NMR Conference (ENC), Asiloma, Calfornia, March 2008, “Spectroscopy by Integration of Frequency and Time Information (SIFT) and Fast Acquisition of Dark Spectra (FADS)” Upgraded from poster
5. P-Chem Seminar, Brandeis University, April 2006, “Spectral Fitting for Signal Assignment and Structural Analysis of Uniformly 13C-Labeled Solid Proteins by Simulated Annealing Based on Chemical Shifts and Spin Dynamics”
6. International Conference on Magnetic Resonance in Biological Systems (ICMRBS), Hyderabard, India, February 2005, “Novel Solid-State NMR Approach to Structural Analysis of Proteins: Application to Phoborhodopsin Transducer pHtrII in Lipid Membrane Environment”
7. The Japan-Korea Bilateral Symposium on Biological NMR, Seoul Korea, Octover 2003, “Novel Solid-State NMR Approach to Structural Analysis of Proteins”

DOMESTIC TALKS

Invited
20. 蛋白質科学会ワークショップ「Novel NMR techniques for diversified protein analysis」、Zoom (June, 2021)
19. 大阪大学蛋白質研究所セミナー「生体膜上の生物科学」、吹田 (March, 2021)

18. 第58回 NMR討論会、川崎 (November, 2019)
17. 第一回高出力遠赤外光・分子物質科学研究会、福井大学 (June, 2019)
16. IAS Seminar, Future of Solid-State DNP NMR, OIST, Okinawa (February, 2019)
15. 理研シンポジウム「タンパク質構造・凝集」、理研和光 (January, 2019)

14. 固体材料フォーラム、産総研、つくば (July, 2018)
13. BINDSシンポジウム、理研、横浜 (May, 2018)

12. 超超プロジェクトDNP-NMRワークショップ、産総研触媒化学融合研究センター、つくば (November, 2017)
11. 蛋白研セミナー「高分極核スピンが拓く未来—DNP法の最前線—」、大阪大学 (August, 2017)
10. 第18回固体NMR技術交流会、群馬大学 (March, 2017)
9. 第72回物理学会年会「核偏極技術の進展とその応用」、大阪 (March, 2017)

8. 7. 第42回内藤コンファレンス 生命科学に革命をもたらす最先端構造生物学、北海道 (October, 2016)
6. 理研シンポジウム 分子構造解析2016:MS とNMR の基礎と実践、理化学研究所、和光 (May, 2016)

5. 日本生化学会、国立京都国際会館 (August, 2014)
4. 固体NMR・材料フォーラム、兵庫県民会館 (2014)
3. 非線形サンプリングシンポジウム, 名古屋大学 (2014)

2. RRR workshop, 東京 (January 2012)
1. 第48回固体材料フォーラム、つくば (October 2010)

PAPERS

2021
1. Y. Matsuki, T. Kobayashi, J. Fukazawa, F. A. Perras, M. Pruski and T. Fujiwara, “Efficiency Analysis of Helium-Cooled MAS DNP: Case Studies of Surface-Modified Nanoparticles and Homogeneous Small-Molecule Solutions">Phys. Chem. Chem. Phys. C, 23, 4919- (2021)
DOI: 10.1039/d0cp05658h

2020
1. Y. Matsuki, T. Sugishita, and T. Fujiwara, “Surface-Only Spectroscopy for Diffusion-Limited Systems using Ultra-Low Temperature DNPMASNMR at 16.4 T", J. Phys. Chem. C, 124, 18609- (2020)
doi/10.1021/acs.jpcc.0c04873
2. T. Kanda, M. Kitawaki, T. Arata, Y. Matsuki, and T. Fujiwara, “Structural analysis of cross-linked poly(vinyl alcohol) using high-field DNP-NMR, RSC Advances, 10, 8039 (2020)
https://doi.org/10.1016/j.ssnmr.2019.02.001

2019
1. T. Sugishita, Y. Matsuki, and T. Fujiwara, “Absolute 1H Polarization Measurement with a Spin-Correlated Component of Magnetization by Hyperpolarized MAS-DNP Solid-State NMR, Solid-State NMR, 99, 20-26 (2019)
https://doi.org/10.1016/j.ssnmr.2019.02.001
2. (Review) Y. Matsuki, and T. Fujiwara, “High-Field DNP Using Closed-Cycle Helium MAS System" , JEOL News, 54, 46-52 (2019)
https://www.jeol.co.jp/en/download_jeolnews.html
3. (総説) 松木 陽, 藤原敏道, “循環型極低温ヘリウムMASを用いる高磁場DNPーNMR" , 日本電子News, 51, 16-21 (2019)
https://www.jeol.co.jp/download_jeolnews.html


2018
1. Y. Matsuki, and T. Fujiwara, “Cryogenic Platforms and Optimized Sensitivity”, High-Frequency Dynamic Nuclear Polarization Nuclear Magnetic Resonance (DNP NMR) Spectroscopy, Edited by Robert Griffin (MIT), Shimon Vega (Weizmann Institute), Bjorn Corzilius (Goethe University) and Vladimir Michaelis (University of Alberta), Wiley (2018)

2017
1. E. M. Khutoryan, T. Idehara, A. N. Kuleshov, Y. Tatematsu, Y. Yamaguchi, Y. Matsuki, and T. Fujiwara, "Simultaneous Stabilization of Gyrotron Frequency and Power by PID Double Feedback Control on the Acceleration and Anode Voltages", J. Infrared. Milli. Terahz. Waves , in press (2017)
2. Y. Matsuki, and T. Fujiwara, “Advances in High-Field DNP Methods”,Chapter 4 (pp.91-134), Experimental Approaches of NMR Spectroscopy: Methodology and Application to Life Science and Materials Science, Edited by The Nuclear Magnetic Resonance Society of Japan, Springer(2017)
ISBN-10: 9811059659 / ISBN-13: 978-9811059650

2016
1. Y. Matsuki, T. Idehara and T. Fujiwara, "Advanced instrumentation for DNP-enhanced MAS NMR for higher magnetic fields and lower temperatures", J. Magn. Reson. , 264, 107-115 (2016)
2. K. Ueda, Y. Matsuki, T. Fujiwara, Y. Tatematsu, I. Ogawa, and T. Idehara, "Further characterization of 394-GHz gyrotron FU-CW GII with additional PID control system for 600-MHz-DNP-SSNMR spectroscopy", J. Infrared. Milli. Terahz. Waves , 37, 825-836 (2016)
3. T. Idehara, E. M. Khutoryan, I. Ogawa, Y. Matsuki, and T. Fujiwara, "Modulation and Stabilization of the Output Power and Frequency of FU Series Gyrotrons", Int. J. Terahz. Sci. Tech , 9, 117-130 (2016)

2015
1. Y. Matsuki, S. Nakamura, S. Fukui, H. Suematsu and T. Fujiwara, "Closed-cycle cold helium magic-angle spinning for sensitivity-enhanced multi-dimensional solid-state NMR", J. Magn. Reson. , 259, 76-81 (2015); JMR most downloaded paper award, 2015 Q3; Selected for the journal cover (October, 2015)
2. T. Idehara, E. M. Khutoryan, Y. Tatematsu, Y. Yamaguchi, A. N. Kuleshov, O. Dumbrajs, Y. Matsuki, and T. Fujiwara, "High-speed frequency modulation of a 460 GHz gyrotron for enhancement of 700 MHz DNP-NMR spectroscopy", J. Infrar. Millim. Terahz. Waves, 36, 819-829 (2015)
3. T. Idehara, Y. Tatematsu, Y. Yamaguchi, E. M. Khutoryan, A. N. Kuleshov, K. Ueda, Y. Matsuki, and T. Fujiwara, "The development of 460 GHz gyrotrons for 700 MHz DNP-NMR spectroscopy", J. Infrar. Millim. Terahz. Waves, 36, 613-627 (2015)

2014
1. R. Ikeda, Y. Yamaguchi, Y. Tatematsu, T. Idehara, I. Ogawa, T. Saito, Y. Matsuki, and T. Fujiwara, "Broadband Continuously Tunable Gyrotron for 600 MHz DNP-NMR Spectroscopy", Plasma Fusion Res., 9, 1206058 (2014)

2012
1. Y. Matsuki, K. Ueda, T. Idehara, R. Ikeda, I. Ogawa, S. Nakamura, M. Toda, T. Anai, and T. Fujiwara, "Helium-Cooling and -Spinning Dynamic Nuclear Polarization for Sensitivity-Enhanced NMR at 14T and 30K", J. Magn. Reson. , 225, 1-9 (2012)
2. T. Idehara, J. C. Mudiganti, L. Agusu, T. Kanemaki, I. Ogawa, T. Fujiwara, Y. Matsuki, K. Ueda, "Development of a Compact sub-THz Gyrotron FU CW CI for Application to High Power THz Technologies", J. Infrar. Millim. Terahz. Waves, 33, 724-744 (2012)
3. Yoh Matsuki, Keisuke Ueda, Toshitaka Idehara, Ryosuke Ikeda, Kosuke Kosuga, Isamu Ogawa, Shinji Nakamura, Mitsuru Toda, Takahiro Anai, and Toshimichi Fujiwara, "Application of Continuously Frequency-Tunable 0.4 THz Gyrotron to Dynamic Nuclear Polarization for 600 MHz Solid-State NMR", J. Infrar. Millim. Terahz. Waves, 33, 745-755 (2012)


2011
1. Yoh Matsuki, Tsuyoshi Konuma, Toshimichi Fujiwara, and Kenji Sugase, "Boosting Protein Dynamics Studies Using Quantitative Non-Uniform Sampling NMR Spectroscopy", J. Phys. Chem. B, 115, 13740-13745 (2011)
2. Judith Herzfeld, Danielle Rand, Yoh Matsuki, Eugenio Daviso, Melody Mak-Jurkauskas, and Irena Mamajanov, "Molecular Structure of Humin and Melanoidin via Solid State NMR", J. Phys. Chem. B, 115, 5741-5745 (2011)
3. Emilio A. Nanni, Alexander B. Barnes, Yoh Matsuki, Paul P. Woskov, Bjorn Corzilius, Robert G. Griffin, and Richard J. Temkin, "Microwave Field Distribution in a Magic Angle Spinning Dynamic Nuclear Polarization NMR Probe, J. Magn. Reson, 210, 16-23 (2011)


2010
1. Yoh Matsuki, Matthew T. Eddy, Robert G. Griffin and Judith Herzfeld, "Rapid Three-Dimensional MAS NMR Spectroscopy at Critical Sensitivity", Angew. Chem. Int. Ed. 122, 9401-9404 (2010) Angewandte designated this paper as "VIP (very important paper)"
2. Alexander B. Barnes, Bjo"rn Corzilius, Melody L. Mak-Jurkauskas, Loren B. Andreas, Vikram S. Bajaj, Yoh Matsuki, Marina L. Belenky, Johan Lugtenburg, Jagadishwar R. Sirigiri, Richard J. Temkin, Judith Herzfeld and Robert G. Griffin, "Resolution and polarization distribution in cryogenic DNP/MAS experiments", Phys. Chem. Chem. Phys. 12, 5861-5867(2010)
3. Yoh Matsuki, Hiroki Takahashi, Keisuke Ueda, Toshitaka Idehara, Isamu Ogawa, Mitsuru Toda, Hideo Akutsu and Toshimichi Fujiwara, "Dynamic Nuclear Polaraization Experiments at 14.1 T for Solid-State NMR", Phys. Chem.Chem. Phys. 12, 5799-5803(2010)
4. Toshitaka Idehara, Kousuke Kosuga, La Agusu, Ryosuke Ikeda, Isamu Ogawa, Teruo Saito, Yoh Matsuki, Keisuke Ueda and Toshimichi Fujiwara, "Continuously Frequency Tunable High Power Sub-THz Radiation Source-Gyrotron FU CWVI for 600MHz DNP-NMR spectroscopy", J.Infrared Millim.Terahz.Waves,31, 775-790 (2010)


2009
1. Yoh Matsuki, Thorsten Maly, Olivier Ouari, Hakim Karoui, Francois Le Moigne, Egon Rizzato, Sevdalina Lyubenova, Judith Herzfeld, Thomas Prisner, Paul Tordo and Robert G. Griffin, "Dynamic nuclear polarization using a rigid biradical", Angew. Chem. Int. Ed. 48, 4996-5000 (2009)
2. Yoh Matsuki, Matthew T. Eddy, and Judith Herzfeld, "Spectroscopy by integration of frequency and time domain information for fast acquisition of high-resolution dark spectra", J. Am. Chem. Soc. 131, 4648-4656 (2009)
3. Alexander B. Barnes, Melody L. Mak-Jurkauskas, Yoh Matsuki, Vikram S. Bajaj, Patrick C.A. van der Wel, Ronald DeRocher, Jeffrey Bryant, Jagadishwar R. Sirigiri, Richard J. Temkin, Johan Lugtenburg, Judith Herzfeld, Robert G. Griffin, "Cryogenic sample exchange NMR probe for magic angle spinning dynamic nuclear polarization", J. Magn. Reson. 198 , 261-270 (2009)

2007
1. Matsuki Y.,Akutsu H., Fujiwara T. "Spectral Fitting for Signal Assignment and Structural Analysis of Uniformly 13C-Labeled Solid Proteins by Simulated Annealing Based on Chemical Shifts and Spin Dynamics" J. Biomol. NMR 38,325-339(2007)

2006
1. Iwata K., Fujiwara T., Matsuki Y., Akutsu H., Takahashi S., Naiki H., Goto Y. "3D Structure of amyloid protofilaments of b2-microglobulin fragment probed by solid-state NMR" Proc. Natl. Acad. Sci. USA 103, 18119-18124
2. Kobayashi M., Matsuki Y., Yumen I., Fujiwara T., Akutsu H. "Signal assignment and secondary structure analysis of a uniformly [13C, 15N]-labeled membrane protein, H+-ATP synthase subunit c, by magic-angle spinning solid-state NMR" J. Biomol. NMR 36, 279-293 (2006)

2004
1. Matsuki Y., Akutsu H., Fujiwara T. "Precision 1H-1H distance measurement via 13C NMR signals: utilization of 1H-1H double-quantum dipolar interactions recoupled under magic angle spinning conditions" Magn. Reson. Chem. 42, 291-300 (2004)

2003
1. Matsuki Y., Akutsu H., Fujiwara T. "Band-Selective Recoupling of Homonuclear Double-Quantum Dipolar Interaction with a Generalized Composite 0 Degrees Pulse: Application to 13C Aliphatic Region-Selective Magnetization Transfer in Solids" J. Magn. Reson. 162, 54-66 (2003)

OTHER

In 1996, back-packing traveled throughout the west Europe (United Kingdom, Netherlands, France, Germany, Switzerland, and Italy).
Three-month in-country experience at Strasbourg, France in 1998.
Fluent in English.
Fluent in French. Crazy about French literature (especially on the avant-gardes).
Like swimming, running and playing tennis.
Also like listening Gustav Mahler, Frank Zappa and Joe Pass.
Prefer Ales to Pilsners.