|Professor of Donated Fund Research||Kiyotoshi SEKIGUCHI|
|Assistant Professor of Donated Fund Research||Kazumasa FUJITA|
Our long-term goal is to understand the molecular mechanisms defining morphogenetic interactions of cells with their surrounding microenvironment, i.e., extracellular matrices. We are particularly interested in the roles of the basement membrane in histogenesis/organogenesis, with an emphasis on the adhesive interactions of cells with basement membrane proteins through the integrin family of cell adhesion receptors and the resulting signaling events that regulate proliferation, differentiation, apoptosis, and motility of cells. An emphasis is also given to the development of culture substrates suitable for efficient expansion as well as directed differentiation of human pluripotent stem cells in regenerative medicine.
Current Research Programs
1. Spatiotemporal customization of the basement membrane composition during mammalian development.
2. Mechanistic basis of the interactions of laminins with integrins.
3. Development of culture substrates optimized for human pluripotent stem cells and tissue stem cells.
4. Functions of polydom, an extracellular matrix protein involved in lymphatic development.
1. Polydom is an extracellular matrix protein involved in lymphatic vessel remodeling. Morooka, N., et al. Circ. Res. 120:1276-1288 (2017).
2. Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Ohta, R., et al. Sci. Rep. 6:35680 (2016) doi: 10.1038/srep35680.
3. Molecular basis of the ligand-binding specificity of alphavbeta8 integrin. Ozawa, A., et al. J. Biol. Chem., 291:11551-1165 (2016).
4. Molecular basis of laminin-integrin interactions. Yamada, M. and Sekiguchi, K. Curr. Top. Membr. 76:197-229 (2015)
5. A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Nakagawa, M., et al. Rep., 4: 3594 (2014) doi: 10.1038/srep03594.
6. Basement membrane assembly of the integrin alpha8beta1 ligand nephronectin requires Fraser syndrome-associated proteins. Kiyozumi, D., et al. J. Cell Biol., 197:677-689 (2012)