Laboratory of Nuclear Network
Organisms promptly and appropriately respond to various environmental changes for their survival. In order to respond to various forms of stress, cells have developed signal transduction cascades for each stress. We will clarify the molecular bases for networks of proteins or cellular signals, focusing on the key proteins that are commonly involved in several distinct signal transductions. Our current interests are crosstalk of signal transductions for chromosome maintenance and those for nutrient recognition. Our study will contribute to understanding the mechanisms of chromosome disease, cancer or diabetes.
Current Research Programs
- Analysis of molecular functions of telomere-binding proteins.
- Analysis of functional networks among the chromosomal non-coding regions including telomeres.
- Analysis of molecular bases for Tel2-PIKK network.
- Xue, J., Chen, H., Wu, J., Takeuchi, M., Inoue, H., Liu, Y., *Chen, Y., *Kanoh, J., and *Lei, M. Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex. (2017) Cell Res.,27: 1503-1520. (* Co-corresponding authors)
- Tashiro, S., Nishihara, Y., Kugou, K., Ohta, K., and Kanoh, J. Subtelomeres constitute a safeguard for gene expression and chromosome homeostasis. (2017) Nucleic Acids Res., 45: 10333-10349. Selected as a Breakthrough Article.
- Fujita, I., Tanaka, M., and Kanoh, J. Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe. (2012) PLoS ONE, 7: e49151.
- Fujita, I., Nishihara, Y., Tanaka, M., Tsujii, H., Chikashige, Y., Watanabe, Y., Saito, M., Ishikawa, F., Hiraoka, Y., and Kanoh, J. Telomere-nuclear envelope dissociation promoted by Rap1 phosphorylation ensures faithful chromosome segregation. (2012) Curr. Biol., 22: 1932-1937.
- Chen, Y., Rai, R., Zhou, Z-R., Kanoh, J., Ribeyre, C., Yang, Y., Zheng, H., Damay, P., Wang, F., Tsujii, H., Hiraoka, Y., Shore, D., Hu, H-Y., Chang, S. and Lei, M. A conserved motif within RAP1 plays diversified roles in telomere protection and regulation in different organisms. (2011) Nature Struct. Mol. Biol., 18: 213-221.
- Miyoshi, T., Kanoh, J., Saito, M. and Ishikawa, F. Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length. (2008) Science, 320: 1341-1344.
- Shikata, M., Ishikawa, F. and Kanoh, J. Tel2 is required for the activation of Mrc1-mediated DNA replication checkpoint. (2007) J. Biol. Chem., 282: 5346-5355.
- Kanoh, J.*, Sadaie, M. Urano T. and Ishikawa, F. Telomere-binding protein Taz1 establishes Swi6 heterochromatin independently of RNAi at telomeres. (2005) Curr. Biol., 15: 1808-1819.
- Kanoh, J. and Ishikawa, F. spRap1 and spRif1, recruited to telomeres by Taz1, are essential for telomere function in fission yeast. (2001) Curr. Biol., 11: 1624-1630.