Laboratory of Advanced Protein Characterization

Member

Director Atsushi NAKAGAWA
Junichi TAKAGI
Toshifumi TAKAO
Associate Professor Yohei MIYANOIRI
Eiki YAMASHITA
Assistant Professor Akifumi HIGASHIURA
Toshihiko SUGIKI
Technical Staff Naoko NORIOKA

 

Correspondence

Tel
Fax
E-mail
URL

Research

Synchrotron Radiation Research Group

【Member】
Professor    Atsushi NAKAGAWA
Assistant Professor    Eiki YAMASHITA
Assistant Professor    Akifumi HIGASHIURA

【Correspondence】
Tel:  06-6879-8635
Fax: 06-6879-4313
E-mail:
URL: http://www.protein.osaka-u.ac.jp/rcsfp/supracryst/jp/info/bl44xu.html

【Research】
We are working on development of new methodologies for X-ray protein crystallography using synchrotron radiation beamline for biological macromolecular assemblies at SPring-8 (BL44XU).

  1. Development of methodologies for X-ray crystal structure determination of biological macromolecular assemblies using synchrotron radiation
  2. Structural studies on membrane proteins and macromolecular complexes

NMR Research Group

【Member】
Assistant Professor    Naotoshi MIMURA
Specially Appointed Assist. Prof.    Toshihiko SUGIKI

【Correspondence】
Tel: 06-6879-4334
Fax: 06-6879-8600
E-mail:
URL: http://www.protein.osaka-u.ac.jp/rcsfp/structure/nmr/index_jap.html

【Research】
Nuclear magnetic resonance (NMR) is a powerful tool for the analyses of protein structures and dynamics at atomic resolutions. Our group determines the three-dimensional structures of proteins using NMR, observes the interactions between proteins and ligands, studies dynamics of proteins, and develops methodology of related NMR techniques.

Electron Microscopy Group

【Member】
Professor   Junichi TAKAGI

【Correspondence】
Tel: 06-6879-8607
Fax: 06-6879-8609
E-mail:
URL: http://www.protein.osaka-u.ac.jp/rcsfp/synthesis/em/iwasaki.html

【Research】
We are engaged in a research & development of EM techniques for the purpose of understanding the molecular architecture in the context of the real life. Technologies being developed include a novel molecular labeling method and hybrid analysis strategies that combine EM imaging with computer simulation, biochemistry, X-ray crystallography, and XFEL.

Molecular Analysis Group

【Member】
Professor   Toshifumi TAKAO
Professor   Atsushi NAKAGAWA
Professor   Junichi TAKAGI
Associate Professor   Takeshi SATO
Technical Staff   Naoko NORIOKA

【Correspondence】
Tel: 066-6878-4324
Fax: 06-6879-4313
Email:
URL:

【Research】
This group is working on analysis of the primary structure of proteins and peptides by using conventional mass spectrometers and peptide sequencers. Nowadays we often use a variety of recombinant proteins for structural and functional study of target proteins. To obtain solid results, information on the chemical properties of recombinant proteins of experimental materials, such as terminal sequences and post-translational modifications, is very useful. This group is accumulating various data for such quality control of proteins to make them appropriate for molecular analysis.

Current Research Programs

References

Synchrotron Radiation Research Group

  1. A high-resolution structure of pre-microRNA nuclear export machinery. Okada et al. Science, 326, 1275-1279
  2. Distinguishing between Cl⁻ and O₂²⁻ as the bridging element between Fe³⁺ and Cu²⁺ in resting-oxidized cytochrome oxidase. Suga. et al., Acta Cryst. D67, 742-744 (2011)

NMR Research Group

  1. Binding energetics of ferredoxin-NADP⁺ reductase with ferredoxin and its relation to function. Lee et al. (2011) Chembiochem 12, 2062-2070.
  2. Characterization of DNA-binding activity in the N-terminal domain of the DNA methyltransferase Dnmt3a. Suetake et al. (2011) Biochem. J. 437, 141-148.

Electron Microscopy Group

  1. Comparison of the envelope architecture of E. coli using two methods: CEMOVIS and cryo-electron tomography. Kishimoto-Okada, A. et al. (2010). J.Electron Microsc., 59, 419-426.