Femtosecond X-ray serial crystallography has revolutionized atomic resolution structural investigation by drastically expanding the accessibility to proteins in small-sized crystals, at room temperature and on dynamic reaction. Despite such an enticing opportunity, robust and reliable crystal carrying media are largely lacking. Here, we introduce a grease matrix carrier for protein microcrystals to obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type-3 beyond 2Å resolution under ambient conditions.
IPR concluded an academic exchange agreement with the Center for Protein Science, Peking University in the People’s Republic of China on September 22, 2014. The agreement aims to enhance academic exchange and research collaboration among students and researchers between our institutes.
IPR concluded an academic exchange agreement with National Center for Protein Science, Shanghai on September 1st, 2014. The agreement aims to enhance academic exchange and research collaboration among students and researchers between our institutes, with common goal to advance scientific research in Asia together.
IPR concluded an academic exchange agreement with Institute of Tropical Disease, Airlangga University, Indonesia on August 1st, 2014. The agreement aims to enhance academic exchange and research collaboration among students and researchers between our institutes, with common goal to advance scientific research in Asia together.
Dr. Kohei Takeshita was given the Young Scientist Award of the Protein Science Society of Japan 2014.
Wednesday May 14, 2014 00:00 UTC (Coordinated Universal Time) Spanning the globe from the US, UK, and Japan, the Worldwide Protein Data Bank (wwPDB) organization announces that the Protein Data Bank archive now contains more than 100,000 entries.
Cilia and flagella are formed and maintained by intraflagellar transport (IFT) and play important roles in sensing and moving across species. At the distal tip of the cilia/flagella, IFT complexes turn around to switch from anterograde to retrograde transport, however, the underlying regulatory mechanism is unclear. Here, we identified ICK kinase localization at the tip of cilia as a regulator of ciliary transport. In ICK-deficient mice, we found ciliary defects in neuronal progenitor cells with Hedgehog signal defects. ICK-deficient cells formed cilia with mislocalized Hedgehog signaling components. Loss of ICK caused the accumulation of IFT-A, IFT-B, and BBSome components at the ciliary tips. In contrast, overexpression of ICK induced the strong accumulation of IFT-B but not IFT-A or BBSome components at ciliary tips. In addition, ICK directly phosphorylated Kif3a, while inhibition of this Kif3a phosphorylation affected ciliary formation. Our results suggest that ICK is a Kif3a kinase and essential for proper ciliogenesis in development by regulating ciliary transport at the tip of cilia.