Laboratories & Administration Office

Laboratory of Protein Profiling and Functional Proteomics

member

Director Toshifumi TAKAO
Technical Assistant Aiko KOBATAKE

連絡先

Tel 81-6-6879-4312
Fax 81-6-6879-4312
E-mail

研究概要

Mass spectrometry is a well accepted technique for peptide sequencing and analysis of post-translational modification. In conjunction with accumulating protein and gene sequence databases, we are using state-of-the-art mass spectrometry for large-scale protein identification which is indispensable for proteomics research. We apply the following developed methods to the structural analysis of micro quantities of peptides, proteins, and their related substances.


<Fig.1>
Novel fatty acid modification (palmitoleoyl modification at Ser209) of Wnt protein
essential for Wnt secretion (Takada R. et al. Developmental Cell, 11, 791-801 ,
2006).


<Fig.2>
Automated quantitation of a peptide by a WEB application, Isotopica
(http://coco.protein.osaka-u.ac.jp/Isotopica).
An 18O-stable-isotope-labeled synthetic peptide was spiked into a urinary sample,
and the resultant mixture was measured by MALDI-MS.
(Rapid Commun. Mass Spectrom. 18, 2465, 2004; Nucleic Acids Res. 32, 674, 2004)

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