DNA Methyltransferase
Characterization of DNA-binding activity in the N-terminal domain of DNA methyltransferase Dnmt3a.
Isao Suetake, Yuichi MIshima, Hironobu KImura, Young-Hoo Lee, Yuji Goto, HIdeyuki Takeshimas, Takahisa Ikegami, and Shoji Tajima
Biochem. J. online publication (2011)

Structural insight into maintenance methylation by mouse Dnmt1.
Kohei Takeshita*, Isao Suetake*, Eiki Yamashita, Michihiro Suga, Hirotaka Narita, Atsushi Nakagawa, and Shoji Tajima
Proc. Natl. Acad. Sci. USA. online publication (2011) (* equal contribution)

Recombinant mammalian DNA methyltranasferase activity on model transcriptional gene silencing short RNA-DNA heteroduplex substrates.
Jason P. Ross, Isao Suetake, Shoji Tajima, and Peter L. Molloy.
Biochem. J. 432, 323-332 (2010)

Array-based genomic resequencing of human leukemia.
Yoshihiro Yamashita, Jin Yuan, Isao Suetake, Hiromu Suzuki, Yuichi Ishikawa, Young Lim Choi, Toshihide Ueno, Manabu Soda, Toru Hamada, Hidenori haruta, Yasushi Miyazaki, Shuji Takada, Hitoshi Kiyoi, Etsuro Ito, Tomoki Naoe, Masao Tomonaga, Minoru Toyota, Shoji Tajima, Atsushi Iwama, and Hiroyuki Mano.
Oncogene 29, 3723-3731 (2010)

The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1 delta/epsilon.
Yasunori Sugiyama, Naoya Hatano, Noriyuki Sueyoshi, Isao Suetake, Shoji Tajima, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, and Isamu Kameshita.
Biochem. J. 427, 489-497 (2010)
Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects.
Seiji Takashima, Masanori Takehashi, Jiyoung Lee, Shinichiro Chuma, Masaki Okano, Kenichiro Hata, Isao Suetake, Norio Nakatsuji, Hiroyuki Miyoshi, Shoji Tajima, Hiroyuki Sasaki, Mito Kanatsu-Shinohara, and Takashi Shinohara.
Biol. Rprod. 81, 155-164 (2009)

Cyclin-dependent kinase-like 5 binds and phosphorylates DNA methyltransferase 1.
Isamu Kameshita, Mari, Sekiguchi, Daisuke Hamasaki, Yasunori Sugiyama, Naoya Hatano, Isao Suetake, Shoji Tajima, and Noriyuki Sueyoshi.
Biochem. Biophys. Res. Commun. 377, 1162-1167 (2008)

Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development.
Ryutaro Hirasawa, Hatsune Chiba, Masahiro Kaneda, Shoji Tajima, En Li, Rudolf Jaenisch, and Hiroyuki Sasaki.
Genes. Dev. 22, 1607-1616 (2008)

Mouse@Dnmt3a preferentially methylates linker DNA, and is inhibited by histone H1.
Hideyuki Takeshima, Isao Suetake, Shoji Tajima.
J. Mol. Biol. 383, 810-821 (2008)

The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA
Sharif Jafar, Masahiro Muto, Shin-ichiro Takebayashi, Isao Suetake, Akihiro Iwamatsu, Takaho A. Endo, Yoko Mizutani-Koseki, Kunihiro Okamura, Shoji Tajima, Kohzoh Mitsuya, Masaki Okano, and Haruhiko Koseki.
Nature, 450, 908-912 (2007)

Bmi1 cooperates with Dnmt1 associated protein in gene silencing.
Masamitsu Negishi, Atsunori Saraya, Satoru Miyagi, Kenji Nagao, Yoshimasa Inagaki, Mitsuo Nishikawa, Shoji Tajima, Haruhiko Koseki, Hiroshi Tsuda, Yoshinari Takasaki, Hiromitsu Nakauchi, and Atsushi Iwama.
Biochem. Biophys. Res. Commun. 353, 992-998 (2007)

The amino-terminus of mouse DNA methyltransferase 1 forms an independent domain and binds to DNA with the sequence involving PCNA binding motif.
Isao Suetake, Daichika Hayata, and Shoji Tajima
J. Biochem. 140, 763-776 (2006)

Stimulation effect of Dnmt3L on the DNA methylation activity of Dnmt3a2.
Isao Suetake, Yuuki Morimoto, Takuya Fuchikami, Kuniya Abe, Shoji Tajima
J. Biochem. 140, 553-559 (2006)

Distinct DNA methylation activity of DNA methyltransferases Dnmt3a and Dnmt3b towards the DNA in nucleosome.
Hideyuki Takeshima, Isao Suetake, Hideto Shimahara, Shin-ichi Tate, Kiyoe Ura, and Shoji Tajima
J. Biochem. 139, 503-515 (2006)

Processive methylation of hemimethylated CpG sites by mouse Dnmt1 DNA methyltransferase.
Giedrius Vilkaitis,Isao Suetake, Saulius Klimasauskas, and Shoji Tajima
J. Biol. Chem. 280, 64-72 (2005)

Co-expression of de novo DNA methyltransferase Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos.
Yasuhiro Sakai*, Isao Suetake*, Fuminori Shinozaki, Shohei Yamashina, and Shoji Tajima.
Gene Expression Patterns 5, 231-237 (2004)  (*equal contribution)

Expression of Dnmt3b in mouse hematopoietic progenitor cells and spermatogonia at specific stages.
Daisuke Watanabe, Isao Suetake, Shoji Tajima*, Kazunori Hanaoka.
Gene Expression Patterns 5, 43-49 (2004) (*corresponding author)

DNMT3L stimulates DNA methylation activity of Dnmt3a and Dnmt3b through a direct interaction.
Isao Suetake, Fuminori Shinozaki, Junichi Miyagawa, Hideyuki Takeshima, and Shoji Tajima.
J. Biol. Chem. 279, 27816-27823 (2004)

Distinct enzymatic properties of recombinant mouse DNA methyltransferases Dnmt3a and Dnmt3b.
Isao Suetake, Junko Miyazaki, Chikako Murakami, Hideyuki Takeshima and Shoji Tajima.
J. Biochem. 133, 737-744 (2003)

Monoclonal antibody against Dnmt1 arrests the cell division of Xenopus early stage embryos.
Hideharu Hashimoto, Isao Suetake, and Shoji Tajima.
Exp. Cell Res. 286, 252-262 (2003)

Biochemical fractionation reveals association of DNA methyltransferase (Dnmt) 3b with Dnmt1 and that of Dnmt3a with histone H3 methyltransferase and HDAC1.
Jharna Datta, Kalpana Ghoshal, Sudarshana M. Sharma, Shoji Tajima, and Samson T. Jacob.
J. Cellular Biochem. 88, 855-64 (2003)

PCNA clamp facilitates DNA cytosine methyltransferase 1 activity on hemimetylated DNA.
Tetsuo Iida, Isao Suetake, Shoji Tajima, Hiroshi Morioka, Satoshi Ohta, Chikashi Obuse, and Toshiki Tsurimoto.
Genes to Cells 7, 997-1007 (2002)

Stage- and cell-specific expression of Dnmt3a and Dnmt3b during embryogenesis.
Daisuke Watanabe*, Isao Suetake*, Takashi Tada, and Shoji Tajima.
Mech. Dev. 118, 187-190 (2002) (*equal contribution)

Three Novel DNMT3B Mutations in Japanese Patients with ICF Syndrome.
Hisao Shirohzu, Takeo Kubota, Azumi Kumazawa, Takashi Sado, Takahito Chijiwa, Kouichi Inagaki, Isao Suetake, Shoji Tajima, Keiko Wakui, Yuko Miki, Masatoshi Hayashi, Yoshimitsu Fukushima, and Hiroyuki Sasaki.
American J. Med. Genet. 112, 31-37 (2002)

Exogenous expression of mouse Dnmt3 induces apoptosis in Xenous early embryos.
Hironobu Kimura, Isao Suetake, and Shoji Tajima.
J. Biochem. 131, 933-941 (2002)

Expression of DNA methyltransferase (Dnmt1) in testicular germ cells during development of mouse embryo.
Yasuhiro Sakai, Isao Suetake, Kunihiko Itoh, Michinao Mizugaki, Shoji Tajima, and Shohei Yamashina.
Cell Struct. Funct. 26, 685-691 (2001)

Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases.
Asako Aoki, Isao Suetake, Junichi Miyagawa, Takayuki Fujio, Takahito Chijiwa, Hiroyuki Sasaki, and Shoji Tajima.
Nucleic Acids Res. 29, 3506-3512 (2001)

Xenopus eggs express identical DNA methyltransferase, Dnmt1, as somatic cells. Liheng Shi, Isao Suetake, Tohru Kawakami, Saburo Aimoto, and Shoji Tajima.
J. Biochem. 130, 359-366 (2001)

Proliferation stage-dependent expression of DNA methyltransferase (Dnmt1) in mouse small@intestine.
Isao Suetake, Liheng Shi, Daisuke Watanabe, Masahiko Nakamura, and Shoji Tajima.
Cell Struct. Funct. 26, 79-86 (2001)

Maintenance-type DNA methyltransferase is highly expressed in post-mitotic neurons and localized in the cytoplasmic compartment.
Kouichi Inano, Isao Suetake, Takayuki Ueda, Yasuko Miyake, Masahiko Nakamura, Masahiko Okada, and Shoji Tajima.
J. Biochem. 128, 315 - 321 (2000)

Xenopus maintenance-Type DNA methyltransferase is accumulated and translocated into germinal vesicles of oocytes.
Hironobu Kimura, Isao Suetake, and Shoji Tajima.
J. Biochem. 125, 1175-1182 (1999)

Isolation and expression of a Xenopus laevis DNA methyltransferase cDNA.
Hironobu Kimura, Goshi Ishihara, and Shoji Tajima.
J. Biochem. 120, 1182-1189 (1996)

Overexpression of DNA methyltransferase in myoblast cells accelerates myotube formation.
Hidekazu Takagi, Shoji Tajima, and Akira Asano.
Eur. J. Biochem. 231, 282-291 (1995)

Isolation and expression of a chicken DNA methyltransferase cDNA.
Shoji Tajima, Hideki Tsuda, Nobunao Wakabayashi, Akira Asano, Shigeki Mizuno, and Katsuhiko Nishimori.
J. Biochem. 117, 1050-1057 (1995)

Methylated DNA Binding Protein
Expression, purification and Characterization of methyl DNA binding protein from Bonbyx mori.
Tomohide Uno, Yuka Nomura, Masahiko Nakamura, Atsushi Nakao, Shoji Tajima, Kengo Kanamaru, HIroshi Yamagata, and Yosuke Iwanaga
J. Insect Science 5:8 (2005)

Xenopus MBD3 plays a crucial role in an early stage of development.
Hidetomo Iwano, Masahiko Nakamura, and Shoji Tajima.
Dev. Biol. 268, 416-428 (2004)

A novel DNA binding protein that recognizes methylated c-Myc binding motif.
Isao Suetake, Shoji Tajima, and Akira Asano.
J. Biochem. 118, 244-249 (1995)

Identification of two novel mouse nuclear proteins that bind selectively to a methylated c-Myc recognizing sequence.
Isao Suetake, Shoji Tajima, and Akira Asano.
Nucl. Acids Res. 21, 2125-2130 (1993)

Others
Downregulation of the tumor suppressor Cbp/PAG1 is mediated by epigenetic histone modifications via the MAPK/P13K pathway.
Kei Suzuki, Chitose Oneyama, Hironobu Kimura, Shoji Tajima, and Masato Okada
J. Biol. Chem. in press (2011)

Recombinant Tol2 transposase with activity in Xenopus embryos.
Takashi Shibano, Masatoshi Takeda, Isao Suetake, Koichi Kawakami, Makoto Asashima, Shoji Tajima, Masanori Taira.
FEBS Lett. 581, 4333-4336 (2007)

Ars insulator identified in sea urchin possesses an activity to ensure the transgene expression in mouse cells.
Shoji Tajima, Keiko Shinohara, Maiko Fukumoto, Reiko Zaitsu, Junichi Miyagawa, Shinjiro Hino, Jun Fan, Koji Akasaka, and Masao Matsuoka
J. Biochem. 139, 705-714 (2006)

Isolation of the novel cDNA of a gene of which expression is induced by a demethylating stimulus.
Junichi Miyagawa, Michio Muguruma, Hiroshi Aoto, Isao Suetake, Masahiko Nakamura, and Shoji Tajima.
Gene, 240, 289-295 (1999)

Genomic organization of the mouse AZ1 gene that encodes the protein localized to preacrosomes of spermatids.
Hiroshi Aoto, Yasuko Miyake, Masahiko Nakamura, and Shoji Tajima.
Genomics 40, 138-141 (1997)

Isolation of a novel cDNA that encodes a protein localized to the pre-acrosome region of spermatids.
Hiroshi Aoto, Junji Tsuchida, Yukio Nishina, Yoshitake Nishimune, Akira Asano, and Shoji Tajima.
Eur. J. Biochem. 234, 8-15 (1995)

Review

Regulation and function of DNA methylation in vertebrates.
Shoji Tajima and Isao Suetake
J. Biochem. 123, 993-999 (1998)