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Reviews and Proceedings
Toshihiko Sugiki, Kyoko Furuita, Toshimichi Fujiwara and Chojiro Kojima, Current NMR Techniques for Structure-Based Drug Discovery, Molecules, 23(1), E148(2018)
First Online:12 Jan 2018 , DOI:10.3390/molecules23010148


Akira R. Kinjo, Gert-Jan Bekker, Hiroshi Wako, Shigeru Endo, Yuko Tsuchiya, Hiromu Sato, Hafumi Nishi, Kengo Kinoshita, Hirofumi Suzuki, Takeshi Kawabata, Masashi Yokochi, Takeshi Iwata, Naohiro Kobayashi, Toshimichi Fujiwara, Genji Kurisu and Haruki Nakamura, New tools and functions in Data-out activities at Protein Data Bank Japan(PDBj), Protein Science, 27(1), 95-102 (2017)
Epub: 18 Sep 2017 , DOI:10.1002/pro.3273

Yoshikazu Hattori, David Heidenreich, Yuki Ono, Toshihiko Sugiki, Kei-ichi Yokoyama, Ei-ichiro Suzuki, Toshimichi Fujiwara, and Chojiro Kojima. Protein 19F-labeling using transglutaminase for the NMR study of intermolecular interactions. Journal of Biomolecular NMR, 68(4), 271-279 (2017)
Published online: 29 July 2017 , DOI: 10.1007/s10858-017-0125-6

Keisuke Ikeda, Ayame Horiuchi, Ayako Egawa, Hajime Tamaki, Toshimichi Fujiwara, and Minoru Nakano. Nanodisc-to-Nanofiber Transition of Noncovalent Peptide-Phospholipid Assemblies ACS Omega, 2,2935-2944 (2017)
Accepted: 09 June 2017 ,DOI:10.1021/acsomega.7b00424

Shoko Shinya, Mariana G. Ghinet, Ryszard Brzezinski, Kyoko Furuita, Chojiro Kojima, Sneha Shah, Evgenii L. Govrigin, Tamo Fukamizo. NMR line shape analysis of a multi-state ligand binding mechanism in chitosanase. Journal of Biomolecular NMR, 67(4), 309-319 (2017)
First Online: 09 Apr 2017 ,DOI: 10.1007/s10858-017-0109-6

Bikash Sahoo, Kenta Maruyama, Jyotheeswar Edula, Takahiro Tougan, Yuxi Lin, Young-Ho Lee, Toshihiro Horii, and Toshimichi Fujiwara, Mechanistic and structural basis of bioengineered bovine Cathelicidin-5 with optimized therapeutic activity, Scientific Reports, 7, 44781, 1-16 (2017)
First Online : 21 March 2017 , DOI: 10.1038/srep44781

Young J.Y., Westbrook J.D., Feng Z., Sala R., Peisach E., Oldfield T.J., Sen S., Gutmanas A., Armstrong D.R., Berrisford J.M., Chen L., Chen M., Di Costanzo L., Dimitropoulos D., Gao G., Ghosh S., Gore S., Guranovic V., Hendrickx P.M., Hudson B.P., Igarashi R., Ikegawa Y., Kobayashi N., Lawson C.L., Liang Y., Mading S., Mak L., Mir M.S., Mukhopadhyay A., Patwardhan A., Persikova I., Rinaldi L., Sanz-Garcia E., Sekharan M.R., Shao C., Swaminathan G.J., Tan L., Ulrich E.L., van Ginkel G., Yamashita R., Yang H., Zhuravleva M.A., Quesada M., Kleywegt G.J., Berman H.M., Markley J.L., Nakamura H., Velankar S., and Burley S.K., OneDep: Unified wwPDB System for Deposition, Biocuration, and Validation of Macromolecular Structures in the PDB Archive, Structure, 25(3), 536-545 (2017)
First Online : 9 February 2017 , DOI: 10.1016/j.str.2017.01.004

Kanako Kuwasako, Nobukazu Nameki, Kengo Tsuda, Mari Takahashi, Atsuko Sato, Naoya Tochio, Makoto Inoue, Takaho Terada, Takanori Kigawa, Naohiro Kobayashi, Mikako Shirouzu, Takuhiro Ito, Taiichi Sakamoto, Kaori Wakamatsu, Peter Guntert, Seizo Takahashi, Shigeyuki Yokoyama,and Yutaka Muto, Solution structure of the first RNA recognition motif domain of human spliceosomal protein SF3b49 and its mode of interaction with a SF3b145 fragment, Protein science, 26(2), 280-291 (2017)
First Online : 27 November 2016 , DOI: 10.1002/pro.3080

Yoh Matsuki, Toshimichi Fujiwara, “Advances in High-Field DNP Methods”,Chapter 4 (pp.91-134), Experimental Approaches of NMR Spectroscopy: Methodology and Application to Life Science and Materials Science, Edited by The Nuclear Magnetic Resonance Society of Japan, Springer(2017)
ISBN-10: 9811059659 / ISBN-13: 978-9811059650

Reviews and Proceedings
Toshihiko Sugiki, Naohiro Kobayashi, Toshimichi Fujiwara, Modern Technologies of Solution Nuclear Magnetic Resonance Spectroscopy for Three-Dimensional Structure Determination of Proteins Open Avenues for Life Scientists, Computational and Structural Biotechnology Journal, Accepted : 3 April 2017


T. Idehara, E.M. Khutoryan, I. Ogawa, Y. Matsuki and T. Fujiwara, Modulation and Stabilization of the Output Power and Frequency of FU Series Gyrotrons, Terahertz Science and Technology, 9 (4), ISSN 1941-7411 (2016) , DOI : 10.11906/TST.117-130.2016.12.12

Kenichi Harada, Eiki Yamashita, Kento Inoue, Koji Yamaguchi, Toshimichi Fujiwara, Atsushi Nakagawa, Tsutomu Kawasaki, and Chojiro Kojima, Expression, purification, and crystallization of a plant-specific DUF1110 protein from Oryza sativa, Acta Crystallographica Section F, 72(Pt6), 480-484 (2016) , DOI : 10.1107/S2053230X16007573

Bikash Ranjan Sahoo and Toshimichi Fujiwara, Conformational states of HAMP domains interacting with sensory 1 rhodopsin membrane 2 systems: An integrated all-atom and coarse-grained molecular dynamics simulation approach, Mol. BioSyst., 13, 193-207 (2017) ,
First Online : 11 November 2016 , DOI: 10.1039/C6MB00730A (2016)

Ju Yaen Kim, Misaki Kinoshita, Satoshi Kume, Guy T. Hanke, Toshihiko Sugiki, John T. Ladbury, Chojiro Kojima, Takahisa Ikegami, Genji Kurisu, Yuji Goto, Toshiharu Hase, and Young-Ho Lee, Noncovalent forces tune the electron transfer complex between ferredoxin and sulfite reductase to optimize enzymatic activity., Biochem. J., 473,3837-3854 (2016)
First Online : 22 August 2016 , DOI : 10.1042/BCJ20160658

Bikash Ranjan Sahoo, Toshimichi Fujiwara, Membrane Mediated Antimicrobial and Antitumor Activity of Cathelicidin 6: Structural Insights from Molecular Dynamics Simulation on Multi-microsecond Scale, PLOS ONE, 11(7): e0158702 (2016)
Publication date : 08 July 2016 , DOI : 10.1371/journal.pone.0158702

Nesreen Alsanousi, Toshihiko Sugiki, Kyoko Furuita, Masatomo So, Young-Ho Lee, Toshimichi Fujiwara, and Chojiro Kojima, Solution NMR structure and inhibitory effect against amyloid-β fibrillation of Humanin containing a D-isomerized serine residue, Biochemical and Biophysical Research Communications, 477, 647-653 (2016)
First Online : 25 June 2016 , DOI : 10.1016/j.bbrc.2016.06.114

Masashi Yokochi, Naohiro Kobayashi, Eldon L. Ulrich, Akira R. Kinjo, Takeshi Iwata, Yannis E. Ioannidis, Miron Livny, John L. Markley, Haruki Nakamura, Chojiro Kojima, and Toshimichi Fujiwara, Publication of nuclear magnetic resonance experimental data with semantic web technology and the application thereof to biomedical research of proteins, J. Biomed. Semantics, 7, 16 (2016)
Publication date : 05 May 2016 , DOI : 10.1186/s13326-016-0057-1

Keisuke Ueda, Yoh Matsuki, Toshimichi Fujiwara, Yoshinori Tatematsu, Isamu Ogawa, and Toshitaka Idehara, Further Characterization of 394-GHz Gyrotron FU CW GII with Additional PID Control System for 600-MHz DNP-SSNMR Spectroscopy, J. Infrared Millim. Terahz. Waves,37(9), 825-836 (2016)
First Online : 23 April 2016 , DOI : 10.1007/s10762-016-0276-7

Yuxi Lin, Jozsef Kardos, Mizue Imai, Tatsuya Ikenoue, Misaki Kinoshita, Toshihiko Sugiki, Koichiro Ishimori, Yuji Goto, and Young-Ho Lee, Amorphous aggregation of cytochrome c with inherently low amyloidogenicity is characterized by the metastability of supersaturation and the phase diagram, Langmuir, 32, 2010-2022 (2016)
Publication date(Web) : 29 January 2016 , DOI : 10.1021/acs.langmuir.5b03810

Yoh Matsuki, Toshitaka Idehara, Jun Fukazawa, and Toshimichi Fujiwara, Advanced instrumentation for DNP-enhanced MAS NMR for higher magnetic fields and lower temperatures, J. Magn. Reson., 264, 107-115 (2016)
First Online : 22 January 2016 , DOI : 10.1016/j.jmr

Hajime Tamaki, Ayako Egawa, Kouki Kido, Tomoshi Kameda, Masakatsu Kamiya, Takashi Kikukawa, Tomoyasu Aizawa, Toshimichi Fujiwara and Makoto Demura, Structure determination of uniformly 13C, 15N labeled protein using qualitative distance restraints from MAS solid-state 13C-NMR observed paramagnetic relaxation enhancement, J. Biomol. NMR, 64, 87-101 (2016)
First Online : 04 January 2016 , DOI : 10.1007/s10858-015-0010-0

Young-Ho Lee and Toshihiko Sugiki. Advanced Techniques to Detect Protein-Protein Interactions using Solution Nuclear Magnetic Resonance. Part I: Chemical Shift Perturbation and Residual Dipolar Coupling. Protein-Protein Interactions (PPIs), in press.

Toshihiko Sugiki and Young-Ho Lee. Advanced Techniques to Detect Protein-Protein Interactions using Solution Nuclear Magnetic Resonance. Part II: Cross-Saturation, Paramagnetic Effects, and In-Cell NMR. Protein-Protein Interactions (PPIs), in press.

Toshihiko Sugiki, Toshimichi Fujiwara, and Chojiro Kojima. Cold-Shock Expression System in E. coli for Protein NMR Studies. Methods in Molecular Biology: Heterologous Gene Expression in E. coli, in press.

Reviews and Proceedings
Toshihiko Sugiki, Koh Takeuchi, Ichio Shimada, and Hideo Takahashi, Preparation of isotopically-labeled recombinant proteins by using yeast Kluyveromyces lactis expression system, PSSJ Archives, 9, e082 (2016)


Takenori Dairaku, Kyoko Furuita, Hajime Sato, Yoshinori Kondo, Chojiro Kojima, Akira Ono, and Yoshiyuki Tanaka, Exploring a DNA Sequence for the Three-Dimensional Structure Determination of a Silver(I)-Mediated C-C Base Pair in a DNA Duplex By 1H NMR Spectroscopy, Nucleosides Nucleotides and Nucleic Acids, 34, 877-900 (2015)
First Online : 17 November 2015 , DOI : 10.1080/15257770.2015.1088160

Yoh Matsuki, Shinji Nakamura, Shigeo Fukui, Hiroto Suematsu and Toshimichi Fujiwara, Closed-cycle cold helium magic-angle spinning for sensitivity-enhanced multi-dimensional solid-state NMR, J. Magn. Reson.,259,76-81(2015)
First Online : 03 August 2015 , DOI : 10.1016/j.jmr
(Jouranl of Magnetic Resonance Most-downloaded Publication Award Q3 2015)

T. Idehara, E. M. Khutoryan, Y. Tatematsu, Y. Yamaguchi, A. N. Kuleshov, O. Dumbrajs, Y. Matsuki and T. Fujiwara, High speed frequency modulation of a 460 GHz gyrotron for enhancement of 700 MHz DNP-NMR spectroscopy, J.Infrared Millim. Terahz. Waves, 36(9), 819-829, (2015)
First Online : 20 June 2015 , DOI : 10.1007/s10762-015-0176-2

A. Gutmanas, P.D. Adams, B. Bardiaux, H.M. Berman, D.A. Case, R.H. Fogh, P. Guntert, P.M. Hendrickx, T. Herrmann, G.J. Kleywegt, N. Kobayashi, O.F. Lange, J.L. Markley, G.T. Montelione, M. Nilges, T.J. Ragan, C.D. Schwieters, R. Tejero, E.L. Ulrich, S. Velankar, W.F. Vranken, J.R. Wedell, J. Westbrook, D.S. Wishart, and G.M. Vuister, NMR Exchange Format: a unified and open standard for representation of NMR restraint data Nat. Struct. Mol. Biol., 22(6), 433-434 (2015)
First Online : 03 June 2015 , DOI : 10.1038/nsmb.3041

Misaki Kinoshita, Ju Yaen Kim, Satoshi Kume, Yukiko Sakakibara, Toshihiko Sugiki, Chojiro Kojima, Genji Kurisu, Takahisa Ikegami, Toshiharu Hase, Yoko Kimata-Ariga, and Young-Ho Lee, Physicochemical natures of interfaces control activity of ferredoxin NADP+ reductase through its interprotein interactions with ferredoxin, Biochim. Biophys. Acta, 1847, 1200-1211 (2015)
First Online : 23 May 2015 , DOI : 10.1016/j.bbabio

Takenori Dairaku, Kyoko Furuita, Hajime Sato, Jakub Sebera, Daichi Yamanaka, Hiroyuki Otaki, Shoko Kikkawa, Yoshinori Kondo, Ritsuko Katahira, F. Matthias Bickelhaupt, Celia Fonseca Guerra, Akira Ono, Vladimir Sychrovsky, Chojiro Kojima, and Yoshiyuki Tanaka, Direct detection of the mercury-nitrogen bond in the thymine-HgII-thymine base-pair with 199Hg NMR spectroscopy, Chem. Commun, 51, 8488-8491(2015)
First Online : 13 April 2015 , DOI: 10.1039/C5CC02423D

T. Idehara, Y. Tatematsu, E.M. Khutoryan, A.N. Kuleshov, K. Ueda, Y. Yamaguchi, Y. Matsuki, and T. Fujiwara, The Development of 460 GHz gyrotrons for 700 MHz DNP-NMR spectroscopy, J. Infrared Millim. Terahz. Waves, 36(7), 613-627 (2015)
First Online : 15 March 2015 , DOI : 10.1007/s10762-015-0150-z

Kyoko Furuita, Saori Kataoka, Toshihiko Sugiki, Yoshikazu Hattori, Naohiro Kobayashi, Takahisa Ikegami, Kazuhiro Shiozaki, Toshimichi Fujiwara, and Chojiro Kojima, Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints, J. Biomol. NMR, 61, 55-64 (2015)
First Online : 27 November 2014 , DOI : 10.1007/s10858-014-9882-7

Saori Kataoka, Kyoko Furuita, Yoshikazu Hattori, Naohiro Kobayashi, Takahisa Ikegami, Kazuhiro Shiozaki, Toshimichi Fujiwara, and Chojiro Kojima, 1H, 15N and 13C resonance assignments of the conserved region in the middle domain of S. pombe Sin1 protein, Biomol NMR Assign, 9(1), 89-92 (2015)
First Online : 08 March 2014 , DOI : 10.1007/s12104-014-9550-6

Kentaro Hanada and Toshihiko Sugiki, Characterization of Lipid Transfer Proteins, Neuromethods: Lipidomics, in press.

Shinji Nakamura, Yoh Matsuki,Toshimichi Fujiwara, NMR Probe:Closed-cycle cold helium magic-angle spinning for sensitivity-enhanced multi-dimensional solid-state NMR,
2015-016082, Japan, Jan 29, 2015; 102016000863.6, Germany, Jan 28 2016; 15/008767, USA, Jan 28 2016


Naohiro Kobayashi, A robust method for quantitative identification of ordered cores in an ensemble of biomolecular structures by non-linear multi-dimensional scaling using inter-atomic distance variance matrix, J. Biomol. NMR, 58 (1), 61-67 (2014)

Kazuya Ishikawa, Koji Yamaguchi, Kazuaki Sakamoto, Satomi Yoshimura, Kento Inoue, Seiji Tsuge, Chojiro Kojima, and Tsutomu Kawasaki, Bacterial effector modulation of host E3 ligase activity suppresses PAMP-triggered immunity in rice, Nat. Commun., 12, 5:5430 (2014)

Ken-ichi Kosami, Izuru Ohki, Minoru Nagano, Kyoko Furuita, Toshihiko Sugiki, Yoji Kawano, Tsutomu Kawasaki, Toshimichi Fujiwara, Atsushi Nakagawa, Ko Shimamoto, and Chojiro Kojima, The crystal structure of the plant small GTPase OsRac1 reveals its mode of binding to NADPH oxidase, J. Biol. Chem., 289, 28569-28578 (2014)

Hery Suwito, Jumina, Mustofa, Pratiwi Pudjiastuti, Much Zaenal Fanani, Yoko Kimata-Ariga, Ritsuko Katahira, Toru Kawakami, Toshimichi Fujiwara, Toshiharu Hase, Hasnah Mohd Sirat, and Ni Nyoman Tri Puspaningsih, Design and Synthesis of Chalcone Derivatives as Inhibitors of the Ferredoxin -- Ferredoxin-NADP+ Reductase Interaction of Plasmodium falciparum: Pursuing New Antimalarial Agents, Molecules, 19(12), 21473-21488 (2014)

Yoshinori Tatematsu, Yuusuke Yamaguchi, Toshitaka Idehara, Tatsuru Kawase, Isamu Ogawa, Teruo Saito, and Toshimichi Fujiwara, Characteristics of the mode converter of Gyrotron FU CW GII radiating Gaussian beams in both the fundamental and second harmonic frequency bands, J. Infrared Millim. Terahz. Waves, 35(6-7), 517-524 (2014)

Ryosuke Ikeda, Yuusuke Yamaguchi, Yoshinori Tatematsu, Toshitaka Idehara, Isamu Ogawa, Teruo Saito, Yoh Matsuki, and Toshimichi Fujiwara, Broadband Continuously Frequency Tunable Gyrotron for 600 MHz DNP-NMR Spectroscopy, Plasma and Fusion Research: Rapid Commun., 9, 1206058 (2014)

Yoshinori Tatematsu, Yuusuke Yamaguchi, Toshitaka Idehara, Tatsuru Kawase, Ryoichi Ichioka, Isamu Ogawa, Teruo Saito, and Toshimichi Fujiwara, Development of second harmonic gyrotrons, Gyrotron FU CW GII and Gyrotron FU CW GIII, equipped with internal mode converters, J.Infrared Millim.Terahz Waves, 35(2), 169-178 (2014)

Ken-ichi Kosami, Izuru Ohki, Kokoro Hayashi, Ryo Tabata, Sayaka Usugi, Tsutomu Kawasaki, Toshimichi Fujiwara, Atsushi Nakagawa, Ko Shimamoto, and Chojiro Kojima, Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac1, Acta Crystallographica Section F,70(1), 113-115 (2014)

Hiroshi Yamaguchi, Jakub Sebera, Jiro Kondo, Shuji Oda, Tomoyuki Komuro, Takuya Kawamura, Takenori Dairaku, Yoshinori Kondo, Itaru Okamoto, Akira Ono, Jaroslav V. Burda, Chojiro Kojima, Vladimir Sychrovsky, and Yoshiyuki Tanaka, The structure of metallo-DNA with consecutive T-HgII-T base-pairs explains positive entropy for the metallo-base-pair formation, Nucleic Acids Res., 42(6), 4094-4099 (2014)

Su-Jin Kang, Yasuto Todokoro, Ikuko Yumen, Bo Shen, Iku Iwasaki, Toshiharu Suzuki, Atsushi Miyagi, Masasuke Yoshida, Toshimichi Fujiwara, and Hideo Akutsu, Active-Site Structure of Thermophilic Foc Subunit Ring in Membranes Elucidated by Solid-State NMR, Biophysical J., 106, 390-398 (2014)

Ayako Egawa, Hideo Akutsu, and Toshimichi Fujiwara, 13C-13C Distance Measurements by Polarization Transfer Matrix Analysis of 13C Spin Diffusion in Uniformly 13C-labeled Molecular Complex under Magic-angle Spinning in "Advances in Biological Solid State NMR" Edited by F. Separovic and A. Naito, Royal Society of Chemistry, pp. 36-52. 2014. DOI:10.1039/9781782627449

Reviews and Proceedings
Toshihiko Sugiki, Toshimichi Fujiwara, and Chojiro Kojima, Latest approaches for efficient protein production in drug discovery, Expert Opinion on Drug Discovery ,9(10), 1-16 (2014)


Mayumi Miyazawa-Onami, Koh Takeuchi, Toshiaki Takano, Toshihiko Sugiki, Ichio Shimada, and Hideo Takahashi. Perdeuteration and methyl-selective 1H, 13C-labeling by using a Kluyveromyces lactis expression system. J. Biomol. NMR, 57, 297-304 (2013)

Toshihiko Sugiki and Naoko Utsunomiya-Tate, Site-specific aspartic acid isomerization regulates self-assembly and neurotoxicity of amyloid-β, Biochem. Biophys. Res. Commun., 441, 493-498 (2013)

Jing Wang, Naoya Tochio, Aya Takeuchi, Jun-ichi Uewaki, Naohiro Kobayashi, and Shin-ichi Tate, Redox-sensitive structural change in the A-domain of HMGB1 and its implication for the binding to cisplatin modified DNA, Biochem. Biophys. Res. Commun., 441, 701-706 (2013)

Jakub Sebera, Jaroslav Burda, Michal Straka, Akira Ono, Chojiro Kojima, Yoshiyuki Tanaka, and Vladimir Sychrovsky, Formation of the T-HgII-T Metal-Mediated DNA Base Pair; Proposal and Theoretical Calculation of the Reaction Pathway, Chem. Eur. J., 19, 9884-9894 (2013)

Rei Abe-Yoshizumi, Shiori Kobayashi, Mizuki Gohara, Kokoro Hayashi, Chojiro Kojima, Seiji Kojima, Yuki Sudo, Yasuo Asami and Michio Homma, Expression, purification and biochemical characterization of the cytoplasmic loop of PomA, a stator component of the Na+ driven flagellar motor, Biophysics, 9, 21-29 (2013)

Kenichi Harada, Eiki Yamashita, Atsushi Nakagawa, Takamitsu Miyafusa, Kouhei Tsumoto, Takashi Ueno, Yoshiharu Toyama, and Shigeki Takeda, Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion, Biochim Biophys Acta, 1834, 284-291 (2013)

Suyeon Bak, Su-Jin Kang, Toshiharu Suzuki, Masasuke Yoshida, Toshimichi Fujiwara, and Hideo Akutsu, Improved Purification of Thermophilic FoF1-ATP Synthase c-Subunit Rings and Solid-State NMR Characterization of Them in Different Lipid Membranes, J. Korean Magn. Reson. Soc., 17 (2), 1-9 (2013)

Keisuke Ikeda, Ayako Egawa, and Toshimichi Fujiwara, Secondary Structural Analysis of Proteins Based on 13C Chemical Shift Assignments in Unresolved Solid-State NMR Spectra Enhanced by Fragmented Structure Database, J. Biomol.NMR, 55,189-200 (2013)

Yoshikazu Hattori, Kyoko Furuita, Izuru Ohki, Takahisa Ikegami, Harumi Fukada, Masahiro Shirakawa, Toshimichi Fujiwara, and Chojiro Kojima, Utilization of lysine 13C-methylation NMR for protein-protein interaction studies, J. Biomol. NMR, 55,19-31 (2013)


Yoh Matsuki, Keisuke Ueda, Toshitaka Idehara, Ryosuke Ikeda, Isamu Ogawa, Shinji Nakamura, Mitsuru Toda, Takahiro Anai, and Toshimichi Fujiwara, Helium-Cooling and -Spinning Dynamic Nuclear Polarization for Sensitivity-Enhanced Solid-State NMR at 14 T and 30 K, J. Magn. Reson, 225,1-9 (2012)

Kumiko Kawasaki, Teppei Kanaba, Momoko Yoneyama, Naoko Murata-Kamiya, Chojiro Kojima, Yutaka Ito, Hiroyuki Kamiya, and Masaki Mishima, Insights into substrate recognition by the Escherichia coli Orf135 protein through its solution structure, Biochem Biophys Res Commun, 420, 263-268 (2012)

Kumiko Kawasaki, Momoko Yoneyama, Naoko Murata-Kamiya, Hideyoshi Harashima, Chojiro Kojima, Yutaka Ito, Hiroyuki Kamiya, and Masaki Mishima, 1H, 13C and 15N NMR assignments of the Escherichia coli Orf135 protein, Biomol NMR Assign, 6, 1-4 (2012)

Naohiro Kobayashi, Yoko Harano, Naoya Tochio, Eiichi Nakatani, Takanori Kigawa, Shigeyuki Yokoyama, Steve Mading, Eldon L. Ulrich, John L. Markley, Hideo Akutsu, and Toshimichi Fujiwara, An automated system designed for large scale NMR data deposition and annotation: Application to over 600 assigned chemical shift data entries to the BioMagResBank from the RIKEN Structural Genomics/Proteomics Initiative internal database, J. Biomol. NMR, 53, 311-320 (2012)

Ikumi Kawahara, Kaichiro Haruta, Yuta Ashihara, Daichi Yamanaka, Mituhiro Kuriyama, Naoko Toki, Yoshinori Kondo, Kenta Teruya, Junya Ishikawa, Hiroyuki Furuta, Yoshiya Ikawa, Chojiro Kojima, and Yoshiyuki Tanaka, Site-specific isotope labeling of long RNA for structural and mechanistic studies, Nucleic Acids Research, 40, e7 (2012)

Ikuko Yumen, Iku Iwasaki, Toshiharu Suzuki, Yasuto Todokoro, Kentaro Tanaka, Osamu Okada, Toshimichi Fujiwara, Masasuke Yoshida, and Hideo Akutsu, Purification, Characterization and Reconstitution into Membranes of the Oligomeric c-Subunit Ring of Thermophilic FoF1-ATP Synthase Expressed in E. coli, Protein Expression and Purification, 82, 396-401 (2012)

Toshitaka Idehara, Jagadish C. Mudiganti, La Agusu, Tomohiro Kanemaki, Isamu Ogawa, Toshimichi Fujiwara, Yoh Matsuki, and Keisuke Ueda, Development of a Compact sub-THz Gyrotron FU CW CI for Application to High Power THz Technologies, J. Infrar. Millim. Terahz. Waves, 33, 724-744 (2012)

Yoh Matsuki, Keisuke Ueda, Toshitaka Idehara, Ryosuke Ikeda, Kosuke Kosuga, Isamu Ogawa, Shinji Nakamura, Mitsuru Toda, Takahiro Anai, and Toshimichi Fujiwara, Application of Continuously Frequency-Tunable 0.4 THz Gyrotron to Dynamic Nuclear Polarization for 600 MHz Solid-State NMR, J. Infrar. Millim. Terahz. Waves, 33, 745-755 (2012)


Shino Mizuho, Hirokazu Amida, Naohiro Kobayashi, Shin-Ichi Aizawa, and Shin-Ichi Tate, The NMR Structure of FliK, the Trigger for the Switch of Substrate Specificity in the Flagellar Type III Secretion Apparatus, J. Mol. Biol., 409, 558-573 (2011)

Yuki Sudo, Rikou Tanaka, Toshitatsu Kobayashi, Naoki Kamo, Toshiyuki Kohno, and Chojiro Kojima, Functional expression of a two-transmembrane HtrII protein using cell-free synthesis Biophysics, 7, 51-58 (2011)

Ken-ichiro Taoka, Izuru Ohki, Hiroyuki Tsuji, Kyoko Furuita, Kokoro Hayashi, Tomoko Yanase, Midori Yamaguchi, Chika Nakashima, Yekti Asih Purwestri, Shojiro Tamaki, Yuka Ogaki, Chihiro Shimada, Atsushi Nakagawa, Chojiro Kojima, and Ko Shimamoto, 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen, Nature, 476, 332-335 (2011)

Yoh Matsuki, Tsuyoshi Konuma, Toshimichi Fujiwara, and Kenji Sugase, Boosting Protein Dynamics Studies Using Quantitative Non-Uniform Sampling NMR Spectroscopy, J. Phys. Chem. B, 115 , 13740-13745 (2011)

Keisuke Ikeda, Tomoshi Kameda, Erisa Harada, Hideo Akutsu, and Toshimichi Fujiwara, Combined use of replica-exchange molecular dynamics and magic-angle-spinning solid-state NMR spectral simulations for determining the structure and orientation of membrane-bound peptide, J. Phys. Chem. B, 115, 9327-9336 (2011)

Kyoko Furuita, Shunpei Murata, JunGoo Jee, Satoshi Ichikawa, Akira Matsuda, and Chojiro Kojima, Structural feature of bent DNA recognized by HMGB1, J. Am. Chem. Soc., 133 , 5788-5790 (2011)

Judith Herzfeld, Danielle Rand, Yoh Matsuki, Eugenio Daviso, Melody Mak-Jurkauskas, and Irena Mamajanov, Molecular Structure of Humin and Melanoidin via Solid State NMR, J. Phys. Chem. B,115, 5741-5745 (2011)

Emilio A. Nanni, Alexander B. Barnes, Yoh Matsuki, Paul P. Woskov, Bjorn Corzilius, Robert G. Griffin, and Richard J. Temkin, Microwave Field Distribution in a Magic Angle Spinning Dynamic Nuclear Polarization NMR Probe, J. Magn. Reson. 210, 16-23 (2011)


Kokoro Hayashi, and Chojiro Kojima, Efficient protein production method for NMR using soluble protein tags with cold shock expression vector, J. Biomol. NMR, 48, 147-155 (2010)

Yoh Matsuki, Matthew T. Eddy, Robert G. Griffin, and Judith Herzfeld, Rapid Three-Dimensional MAS NMR Spectroscopy at Critical Sensitivity, Angew. Chem. Int. Ed. 122, 9401-9404 (2010)
Angewandte designated this paper as "VIP (very important paper)"

Yasuto Todokoro, Masatoshi Kobayashi, Takeshi Sato, Toru Kawakami, Ikuko Yumen, Saburo Aimoto, Toshimichi Fujiwara, and Hideo Akutsu, "Structure analysis of membrane-reconstituted subunit c-ring of E. coli H+-ATP synthase by solid-state NMR under MAS", J. Biomol. NMR, 48, 1-11 (2010)

Alexander B. Barnes, Bjo"rn Corzilius, Melody L. Mak-Jurkauskas, Loren B. Andreas, Vikram S. Bajaj, Yoh Matsuki, Marina L. Belenky, Johan Lugtenburg, Jagadishwar R. Sirigiri, Richard J. Temkin, Judith Herzfeld, and Robert G. Griffin, Resolution and polarization distribution in cryogenic DNP/MAS experiments,Phys. Chem. Chem. Phys.12, 5861-5867(2010)

Yoh Matsuki, Hiroki Takahashi, Keisuke Ueda, Toshitaka Idehara, Isamu Ogawa, Mitsuru Toda, Hideo Akutsu, and Toshimichi Fujiwara, Dynamic Nuclear Polaraization Experiments at 14.1 T for Solid-State NMR,Phys. Chem.Chem. Phys.12 , 5799-5803(2010)

Toshitaka Idehara, Kousuke Kosuga, La Agusu, Ryosuke Ikeda, Isamu Ogawa, Teruo Saito, Yoh Matsuki, Keisuke Ueda, and Toshimichi Fujiwara, Continuously Frequency Tunable High Power Sub-THz Radiation Source-Gyrotron FU CWVI for 600MHz DNP-NMR spectroscopy, J.Infrared Millim.Terahz.Waves.31, 775-790(2010)

Hiroki Takahashi, Masatsune Kainosho, Hideo Akutsu, and Toshimichi Fujiwara, 1H-Detected 1H-1H Correlation Spectroscopy of a Stereo-Array Isotope Labeled Amino Acid under Fast Magic-Angle Spinning, J.Magn.Reson.203,253-256(2010)

Hideo Akutsu, Ayako Egawa, and Toshimichi Fujiwara, Atomic structure of the bacteriochlorophyll c assembly in intact chlorosomes from Chlorobium limicola determined by solid-state NMR, Photosynth. Res.104(2),221-231

Kyoko Furuita, JunGoo Jee, Harumi Fukada, Masaki Mishima, and Chojiro Kojima, Electrostatic interaction between oxysterol binding protein and VAMP-associated protein-A revealed by NMR and mutagenesis studies, J. Biol. Chem., 285, 12961-12970 (2010)

Takashi Oda, Hiroshi Hashimoto, Naoyuki Kuwabara, Satoko Akashi, Kokoro Hayashi, Chojiro Kojima, Hann Ling Wong, Tsutomu Kawasaki, Ko Shimamoto, Mamoru Sato, and Toshiyuki Shimizu, The structure of the N-terminal regulatory domain of a plant NADPH oxidase and its functional implications. J. Biol. Chem., 285,1435-1445 (2010)

Hideo Akutsu and Toshimichi Fujiwara, Structure and Function Studies of Energy and Signal Transducing Proteins by Solid-State NMR, Encyclopedia of Magnetic Resonance, eds-in-chief R.K.Harris and R.Wasylishen, eds McDermott, A.E and Polenova, T.John Wiley & Sons Ltd, Chichester, UK, pp517-524(2010))


Yoh Matsuki, Thorsten Maly, Olivier Ouari, Hakim Karoui, Francois Le Moigne, Egon Rizzato, Sevdalina Lyubenova, Judith Herzfeld, Thomas Prisner, Paul Tordo and Robert G. Griffin, Dynamic nuclear polarization using a rigid biradical, Angew. Chem. Int. Ed. 48, 4996-5000 (2009)
Patent Applications:
-M.I.T. Technology Disclosure: #13595
-US Patent: US2010/0249386 A1
-International Patent: WO/2010/108995

Yoh Matsuki, Matthew T. Eddy, and Judith Herzfeld, Spectroscopy by integration of frequency and time domain information for fast acquisition of high-resolution dark spectra, J. Am. Chem. Soc. 131, 4648-4656 (2009)

Alexander B. Barnes, Melody L. Mak-Jurkauskas, Yoh Matsuki, Vikram S. Bajaj, Patrick C.A. van der Wel, Ronald DeRocher, Jeffrey Bryant, Jagadishwar R. Sirigiri, Richard J. Temkin, Johan Lugtenburg, Judith Herzfeld, Robert G. Griffin, Cryogenic sample exchange NMR probe for magic angle spinning dynamic nuclear polarization, J. Magn. Reson. 198 , 261-270 (2009)

Seiji Kojima, Katsumi Imada, Mayuko Sakuma, Yuki Sudo, Chojiro Kojima, Tohru Minamino, Michio Homma and Keiichi Namba, Stator assembly and activation mechanism of the flagellar motor by the periplasmic region of MotB, Mol. Microbiol., 73, 710-718 (2009)



Kobayashi M., Struts A.V., Fujiwara T., Brown M.F., Akutsu H. Fluid mechanical matching of H -ATP synthase subunit ring with lipid membranes revealed by H solid-state NMR. Biophys.J.94,4339-4347(2008).

Takahashi H., Akutsu H., Fujiwara T. A magic-angle-spinning NMR method for H? H distance measurement using coherent polarization transfer in C-labeled organic solids J. Chem. Phys. 129, 154504 (2008).

Todokoro Y., Kobayashi M., Fujiwara T., Akutsu H. Structural analysis of membrane systems by solid-state NMR. in Chemistry, Physics, and Biology in Macromolecular Science ed. T. Sato, 173-182, Osaka University Press, Osaka (2008).


Yagi H.,Kajiwara N.,Tanaka H.,Tsukihara T.,Kato-Yamada Y., Yoshida M.,and Akutsu H. Structures of the Thermophilic F1-ATPase ε Subunit Suggesting ATP-Regulated Arm Motion of its C-Terminal Domain in F1. Proceeding of the National Academy of Sciences of the United States of America, 104, 11233-11238.

Matsuki Y.,Akutsu H., Fujiwara T. Spectral Fitting for Signal Assignment and Structural Analysis of Uniformly 13C-Labeled Solid Proteins by Simulated Annealing Based on Chemical Shifts and Spin Dynamics. Journal of Biomolecular NMR 38,325-339(2007).

Idehara T.,Ogawa I.,La Agusu.,Kanamaki T.,Mitsudo S.,Saito T., Fujiwara T.,and Takahashi H. Development of 394.6 Ghz CW gyrotron (Gyrotron FU CW II) for 600 Mhz DNP/proton NMR. International Journal of Infrared and Millimeter Waves 28, 433-442.

Iimura S., Umezaki T., Takeuchi M., Mizuguchi M., Yagi H., Ogasahara K., Akutsu H., Noda Y., Segawa S., Yutani K. Characterization of the denatured structure of pyrrolidone carboxyl peptidase from a hyperthermophile under nondenaturing conditions: Role of the C-terminal a-helix of the protein in folding and stability. Biochemistry 46, 3664-3672.

Akutsu H., Takayama Y. Functional roles of the heme architecture and its environment in tetraheme cytochrome c. Accounts of Chemical Research 40, 171-178.

Egawa A., Fujiwara T., Mizoguchi T., Kakitani Y., Koyama Y., Akutsu H. Structure of the light-harvesting bacteriochlorophyll c assebmly in chlorosomes from Chlorobium limicola determined by solid-state NMR. Proceedings of the National Academy of Sciences of the United States of America 104, 790-795.

Shen Y., Takayama Y., Wei Y., Harada E., Nakatani E., Akutsu H. Refolding of mis-folded recombinant cytochrome c3 with strong cation exchange chromatography. Journal of Liquid Chromatography & Related Technologies 30, 1051-1059.

Takayama Y., Shen Y., Akutsu H. Process ofmaturation of tetraheme cytochrome c3 in a Shewanella expression system. Journal of Biochemistry (Tokyo) 141, 121-126.

Komi N., Okawa K.,Tateishi Y.,Shirakawa M., Fujiwara T., and Akutsu H. Structural Analysis of Pituitary Adenylate Cyclase-Activating Polypeptides Bound to Phospholipid Membranes by Magic Angle Spinning Solid-State NMR. Biochimica et Biophysica Acta-Biomembranes 1768, 3001-3011.

Fujiwara T., Akutsu H. Secondary Structure Analysis of Protein from Angle Dependent Interactions. Webb G. Ed. Modern Magnetic Resonance, pp731-736, Springer-Verlag.


Iwata K., Fujiwara T., Matsuki Y., Akutsu H., Takahashi S., Naiki H., Goto Y. 3D Structure of amyloid protofilaments of b2-microglobulin fragment probed by solid-state NMR. Proceedings of the National Academy of Sciences of the United States of America 103, 18119-18124.

Kobayashi M., Matsuki Y., Yumen I., Fujiwara T., Akutsu H. Signal assignment and secondary structure analysis of a uniformly [13C, 15N]-labeled membrane protein, H+-ATP synthase subunit c, by magic-angle spinning solid-state NMR. Journal of Biomolecular NMR 36, 279-293.

Son W.-S., Kim W.-J., Kim Y.-J., Suh S.-W., Ikegami T., Akutsu H., Lee B.-J. 1H, 13C and 15N resonance assignments of the pyrazinamidase from Mycobacterium tuberculosis. Journal of Biomolecular NMR 36 (Supplement 5), 60.

Morishita H., Umitsu M., Murata Y., Shibata N., Udaka K., Higuchi Y., Akutsu H., Yamaguchi T., Yagi T., Ikegami T. Structure of the CNR/Protocadherin-a first cadherin domain reveals diversity across cadherin families. Journal of Biological Chemistry 281, 33650-33663.

Harada E., Todokoro Y., Akutsu H., Fujiwara T. Detection of Peptide-Phospholipid Interaction Sites in Bilayer Membranes by 13C NMR Spectroscopy: Observation of 2H/31P- Selective 1H-Depolarization under Magic-Angle Spinning. Journal of the American Chemical Society 128, 10654-10655.

Todokoro Y., Yumen I., Fukushima K., Kang S.-W., Park J.-S., Kohno T., Wakamatsu K., Akutsu H., Fujiwara T. Structure of Tightly Menbrane-Bound Mastoparan-X, A G-Protein- Activating Peptide, Determined by Solid-State NMR. Biophysical Journal 91, 1368-1379.

Yahata N., Ozawa K., Tomimoto Y., Morita K., Komori F., Ogata H., Higuchi Y., Akutsu H. Roles of charged residues in pH-dependent redox properties of cytochrome c3 from Desulfovibrio vulgaris Miyazaki F. BIOPHYSICS 2, 45-56 (online).

Kakitani Y., Nagae H., Mizoguchi T., Egawa A., Akiba K., Fujiwara T., Akutsu H., Koyama Y. Assembly of a Mixture of Isomeric Bchl c from Chlorobium limicola As Determined by Intermolecular 13C-13C Dipolar Correlations: Coexistence of Dimer-Based and Pseudo-Monomer-Based Stackings. Biochemistry 45, 7574-7585.

Takai T., Takaya T., Nakano M., Akutsu H., Nakagawa A., Aimoto S., Nagai K., Ikegami T. Orexin-A is composed of a highly conserved C-terminal and a specific, Hydrophilic N-terminal region, revealing the structural basis of specific recognition by the orexin-1 receptor. Journal of Peptide Science 12, 443-454.

Nosaka A. Y., Nishino J., Fujiwara T., Ikegami T., Yagi H., Akutsu H., Nosaka Y. Effects of thermal treatments on the recovery of adsorbed water and photocatalytic activities of TiO2 photocatalytic Systems. Journal of Physical Chemistry B 110, 8380-8385.

Saitoh T., Ikegami T., Nakayama M., Teshima K., Akutsu H.,Hase T. NMR Study of the Electron Transfer Complex of Plant Ferredoxin and Sulfite Reductase: Mapping the interaction sites of ferredoxin. Journal of Biological Chemistry 281, 10482-10488.

Akagi K., Watanabe J., Hara M., Kezuka Y., Chikaishi E., Yamaguchi T., Akutsu H., Nonaka T., Watanabe T., Ikegami T. Identification of the substrate interaction region of the chitin- binding domain of Streptomyces griseus chitinase C. Journal of Biochemistry (Tokyo) 139, 483-493.

Fujiwara T., Ramamoorthy A. “How Far Can the Sensitivity of NMR Be Increased?”. Annual Reports on NMR Spectroscopy 58, 159-180.

Nakano T., Ikegami T., Suzuki T., Yoshida M., Akutsu H. A New Solution Structure of ATP Synthase Subunit c From Thermophilic Bacillus PS3, Suggesting a Local Conformational Change for H+-Translocation. Journal of Molecular Biology 358, 132-144.

Takayama Y., Kobayashi Y., Yahata N., Saitoh T., Hori H., Ikegami T., Akutsu H. Specific Binding CO to Tetraheme Cytochrome c3. Biochemistry 45, 3163-3169.

Yahata N., Saitoh T., Takayama Y., Ozawa K., Ogata H., Higuchi Y., Akutsu H. Redox Interaction of Cytochrome c3 with [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F. Biochemistry 45, 1653-1662.

Kim S.-M., Yamamoto T., Todokoro Y., Takayama Y., Fujiwara T., Akutsu H. Phospholipid-Dependent Regulation of Cytochrome c3-Mediated Electron Transport across Membranes. Biophysical Journal 90, 506-513.


Umitsu M., Morishita H., Murata Y., Udaka K., Akutsu H., Yagi T., Ikegami T. 1H, 13C and 15N resonance assignment of the first cadherin domain of Cadherin-related neuronal receptor (CNR)/protocadherin a. Journal of Biomolecular NMR 31, 365-366.

Sabchevski S., Idehara T., Mitsudo S., Fujiwara T. Conceptual Design Study of a Novel Gyrotron for NMR/DNP Spectroscopy. International Journal of Infrared and Millimeter Waves 26, 1241-1264.


Yagi H., Tsujimoto T., Yamazaki T., Yoshida M., Akutsu H. Conformational change of H+-ATPase b monomer revealed on segmental isotope labeling NMR spectroscopy. Journal of American Chemical Society 126, 16632-16638.

Iimura S., Yagi H., Ogasahara K., Akutsu H., Noda Y., Segawa S., Yutani K. Unusually slow denaturation and refolding processes of pyrrolidone carboxyl peptidase from a hyperthermophile are highly cooperative: real-time NMR studies.. Biochemistry 43, 11906-11915.

Takayama Y., Harada E., Kobayashi R., Ozawa K., Akutsu H. Roles of Noncoordinated Aromatic Residues in Redox Regulation of Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F. Biochemistry 43, 10859-10866.

Nosaka A. Y., Fujiwara T., Yagi H., Akutsu H., Nosaka Y. Characteristics of Water Adsorbed on TiO2 Photocatalytic Systems with Increasing Temperature as Studied by Solid-State 1H NMR Spectroscopy.

Lee J.-H., Park C.-J. Shin J.-S., Ikegami T., Akutsu H., Choi B.-S. NMR structure of the DNA decamer duplex containing double T-G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex. Nucleic Acids Research 32, 2474-2481.

Saitoh T., Tachibana Y., Higuchi Y., Hori H., Akutsu H. Correlation between the g Tensors and the Nonplanarity of Porphyrin Rings in Desulfovibrio vulgaris Miyazaki F Cytochrome c3, Studied by Single Crystal EPR. Bulletin of the Chemical Society of Japan 77, 357-363.

Fujiwara T., Todokoro Y., Yanagishita H., Tawarayama M., Kohno T., Wakamatsu K., Akutsu H. Signal assignments and chemical-shift structural analysis of uniformly 13C, 15N-labeled peptide, mastoparan-X by multidimensional solid-state NMR under magic-angle spinning. Journal of Biomolecular NMR 28, 311-325.

Matsuki Y., Akutsu H., Fujiwara T. Precision 1H-1H distance measurement via 13C NMR signals: utilization of 1H-1H double-quantum dipolar interactions recoupled under magic angle spinning conditions. Magnetic Resonance in Chemistry 42, 291-300.

Sato M., Shibata N., Morimoto Y., Takayama Y., Ozawa K., Akutsu H., Higuchi Y., Yasuoka N. X-ray induced reduction of the crystal of high-molecular-weight cytochrome c Revealed by microspectrophotometry. Journal of Synchrotron Radiation 11, 113-116.


Ozawa K., Takayama Y., Yasukawa F., Ohmura T., Cusanovich M. A., Tomimoto Y., Ogata H., Higuchi Y., Akutsu H. Roles of the Aromatic Ring of Tyr43 in Tetraheme Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F. Biophysical Journal 85, 3367-3374.

Nosaka A. Y., Fujiwara T., Yagi H., Akutsu H., Nosaka Y. Photocatalytic Reaction Sites at the TiO2 Surface as Studied by Solid-State 1H NMR Spectroscopy. Langmuir 19, 1935-1937.

Nosaka A. Y., Kojima E., Fujiwara T., Yagi H., Akutsu H., Nosaka Y. Photoinduced Changes of Adsorbed Water on a TiO2 Photocatalytic Film As Studied by 1H NMR Spectroscopy. The Journal of Physical Chemistry Part B 107, 12042-12044.

Matsuki Y., Akutsu H., Fujiwara T. Band-Selective Recoupling of Homonuclear Double-Quantum Dipolar Interaction with a Generalized Composite 0 Degrees Pulse: Application to 13C Aliphatic Region-Selective Magnetization Transfer in Solids. Journal of Magnetic Resonance 162, 54-66.

Khitrin A. K., Fujiwara T., Akutsu H. Phase-modulated heteronuclear decoupling in NMR of solids. Journal of Magnetic Resonance 162, 46-53.

Kyogoku Y., Fujiyoshi Y., Shimada I., Nakamura H., Tsukihara T., Akutsu H., Odahara T., Nomura N. Structural genomics of membrane proteins. Account of Chemical Research 36, 199-206.


Harada E., Kumagai J., Ozawa K., Imabayashi S., Tsapin A. S., Nealson K. H., Meyer T. E., Cusanovich M. A., Akutsu H. A directional electron transfer regulator based on heme-chain architecture in the small tetraheme cytochrome c from Shewanella oneidensis. FEBS Letter 532, 333-337.

Harada E., Fukuoka Y., Ohmura T., Fukunishi A., Kawai G., Fujiwara T., Akutsu H. Redox-Coupled Conformational Alternations in Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F on the Basis of its Reduced Solution Structure. Journal of Molecular Biology 319, 767-778.

Sato T., Kawakami T., Akaji K., Konishi H., Mochizuki K., Fujiwara T., Akutsu H., Aimoto S. Synthesis of a membrane protein with two transmembrane regions. Journal of Peptide Science 8, 172-180.

Sugase K., Oyama Y., Kitano K., Akutsu H., Ishiguro M. Structure-activity relationships for mini atrial natriuretic Peptide by proline-scanning mutagenesis and shortening of peptide backbone. Bioorganic & Medicinal Chemistry Letters 12, 1245-1247.

Sugase K., Oyama Y., Kitano K., Iwashita T., Fujiwara T., Akutsu H., Ishiguro M. Designing analogues of mini atrial natriuretic Peptide based o structural analysis by NMR and restrained molecular dynamics. Journal of Medicinal Chemistry 45, 881-887.

-Japanese- Akutsu H., Fujiwara T. Structural Analysis of Membrane Proteins by Solid-State NMR. Tanpakushitsu Kakusan Koso 47, 1144-1151.


Tozawa K., Yagi H., Hisamatsu K., Ozawa K., Yoshida M., Akutsu H. Functions and ATP-binding responses of the twelve histidine residues in the TF1-ATPase b subunit. Journal of Biochemistry (Tokyo) 130, 527-533.

Tsapin Al I., Vandenberghe I., Nealson K. H., Scott J. H., Meyer T. E., Cusanovich M. A., Harada E., Kaizu T., Akutsu H., Leys D., VanBeeumen J. J. Identification of a small tetraheme cytochrome c And a flavocytochrome c As two of the principal soluble cytochromes c in Shewanella oneidensis strain MR1. Applied and Environmental Microbiology 67, 3236-3244.

Ozawa K., Yasukawa F., Fujiwara Y., Akutsu H. A simple, rapid, and highly efficient gene expression system for multiheme cytochromes c. Bioscience, Biotechnology, and Biochemistry 65, 185-189.


Ozawa K., Tsapin A. I., Nealson K. H., Cusanovich M. A., Akutsu H. Expression of a tetraheme protein, Desulfovibrio vulgaris Miyazaki F cytochrome c3, in Shewanella oneidensis MR-1. Applied and Environmental Microbiology 66, 4168-4171.

Fujiwara T., Khandelwal P., Akutsu H. Compound radiofrequency-driven recoupling pulse sequences for efficient magnetization transfer by homonuclear dipolar interaction under magic-angle spinning conditions. Journal of Magnetic Resonance 145, 73-83.

Ozawa K., Meikari T., Motohashi K., Yoshida M., Akutsu H. Evidence for the presence of an F-type ATP synthase involved in sulfate respiration in Desulfovibrio vulgaris. Journal of Bacteriology 182, 2200-2206.


Yagi H., Tozawa K., Sekino N., Iwabuchi T., Yoshida M., Akutsu H. Functional conformation changes in the TF1-ATPase b subunit probed by 12 tyrosine residues. Biophysical Journal 77, 2175-2183.

Kim S., Kiyonaga H., Yamaguchi H., Morozumi H., Fujiwara T., Ando S., Tsuge H., and Akutsu H. Osmotic stability of muramyl dipeptide-bearing liposomes and  molecular miscibility in their membranes . Bulletin of the Chemical Society of Japan 72, 541-548.

Yoshida S., Aso Y., Kojima S., Sakurai S., Fujiwara T., And Akutsu H. Mobility of protein in lyophilized formulations linked to the molecular mobility of polymer excipients, as determined by high resolution 13C solid-state NMR. Pharmaceutical Reserch 16, 1621-1625.

-Japanese- Fujiwara T., Akutsu H. Solid-state nuclear magnetic resonance. Tanpakushitsu Kakusan Koso 44, 582-589.


Fujiwara T., Shimomura T., Ohigashi Y., and Akutsu H. Multidimensional solid-state nuclear magnetic resonance for determining the dihedral angle from the correlation of 13C-1H and 13C-13C dipolar interactions under magic-angle spinning conditions . Journalof Chemical Physics 109, 2380-2393.

Ohmura T., Harada E., Fujiwara T., Kawai G., Watanabe K., and Akutsu H. Paramagnetic inversion of the sign of the interference contribution to the transverse relaxation of the imido protons of the coordinated imidazoles in the uniformly 15N Labeled cytochrome c3. Journal of Magnetic Resonance 131, 367-372.

Kim S., Shin K., Fujiwara T., and Akutsu H. The interactions of ferric and ferrous cytochrome c with cardiolipin in phospholipid membranes studied by solid-state 2H and 31P NfMR. Journal of Molecular Structure 441, 183-188.