生体分子解析研究室 N末端アミノ酸配列受託分析 509、510号室の所内用共通設備 メンバー 蛋白研HP English

Peptide mass fingerprinting using MALDI-TOF MS

Laboratory for Biomolecular Analysis, Institute for Protein Research, Osaka Univ.
Back to "Examples and Protocols"
2023.9.29 updated
Analysed on:
Brker FlexAnalysis
Bruker Biotools
Mascot Distiller
Mascot Distiller (peak list)
Mascot Server (Summary)
Mascot Server (Protein view)

Sample preparation: In-gel trypsin digestion of 1 pmol BSA

Mass spectometer: Autorflex maX (Bruker)

Operation mode: Reflector, positive

Matrix: 7 mg/ml alpha-CHCA in 50% acetonitrile/0.1% TFA

Calibration standard: Bruker Peptide standard II