Measurement of protein mass on MALDI-TOF MS (linear mode)
Laboratory for Biomolecular Analysis, Institute for Protein Research, Osaka Univ.
Mass spectometer: Autoflex maX (Bruker)
Operation mode: Linear, positive
Sample: Glycogen phosphorylase (Rabbit), muscle form (Sigma-Aldrich, #P00489), 0.5 mg/ml
Theoretical average mass: 97430.38 (M+H)
Matrix: 10 mg/ml sinapinic acid in 50% acetonitrile/0.1% TFA
Calibration standard: Protein standard II (Bruker)
