N-terminal sequencing of protein on PVDF membrane
Laboratory for Biomolecular Analysis, Institute for Protein Research, Osaka Univ.
Sample protein: 5 pmol of Bovine Serum Albumin
Sample apply: Dot blot on a PVDF membrane (Immobilon PSQ, Millipore).
Sequencer: Shimadzu PPSQ-53A Gradient System