Okumura's page

Publications

Original papers

Okumura N, Takao T. The zinc form of carnosine dipeptidase 2 (CN2) has dipeptidase activity but its substrate specificity is different from that of the manganese form. Biochem Biophys Res Commun. (2017) 494, 484-490.

Katano T, Fukuda M, Furue H, Yamazaki M, Abe M, Watanabe M, Nishida K, Yao I, Yamada A, Hata Y, Okumura N, Nakazawa T, Yamamoto T, Sakimura K, Takao T, Ito S. Involvement of Brain-Enriched Guanylate Kinase-Associated Protein (BEGAIN) in Chronic Pain after Peripheral Nerve Injury. eNeuro. (2016) Oct 17;3(5).pii: ENEURO.0110-16.2016.

Okumura N, Tamura J, Takao T. Evidence for an essential role of intradimer interaction in catalytic function of carnosine dipeptidase II using electrospray-ionization mass spectrometry. Protein Sci. (2016) 25, 511-22.

Sakakibara K, Eiyama A, Suzuki SW, Sakoh-Nakatogawa M, Okumura N, Tani M, Hashimoto A, Nagumo S, Kondo-Okamoto N, Kondo-Kakuta C, Asai E, Kirisako H, Nakatogawa H, Kuge O, Takao T, Ohsumi Y, Okamoto K. Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling. EMBO J. (2015) Nov 3;34(21):2703-19.

Okumura N, Tsujihata M, Momohara C, Yoshioka I, Suto K, Nonomura N, Okuyama A, Takao T. Diversity in protein profiles of individual calcium oxalate kidney stones.PLoS One. (2013) Jul 9;8(7):e68624.

Kimura M, Okumura N, Kose S, Takao T, Imamoto N. Identification of cargo proteins specific for importin-β with importin-α applying a stable isotope labeling by amino acids in cell culture (SILAC)-based in vitro transport system. J Biol Chem. (2013) Aug 23;288(34):24540-9.

Kimura M, Kose S, Okumura N, Imai K, Furuta M, Sakiyama N, Tomii K, Horton P, Takao T, Imamoto N. Identification of cargo proteins specific for the nucleocytoplasmic transport carrier transportin by combination of an in vitro transport system and stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics. Mol Cell Proteomics. (2013) Jan;12(1):145-57.

Kim C, Aono S, Marubashi S, Wada H, Kobayashi S, Eguchi H, Takeda Y, Tanemura M, Okumura N, Takao T, Doki Y, Mori M, Nagano H. Significance of alanine aminopeptidase N (APN) in bile in the diagnosis of acute cellular rejection after liver transplantation. J Surg Res. (2012) Jun 1;175(1):138-48.

Kishi S, Shimoke K, Nakatani Y, Shimada T, Okumura N, Nagai K, Shin-Ya K, Ikeuchi T. Nerve growth factor attenuates 2-deoxy-d-glucose-triggered endoplasmic reticulum stress-mediated apoptosis via enhanced expression of GRP78. Neurosci Res. 66, 14-21(2010)

Kawaguchi C, Isojima Y, Shintani N, Hatanaka M, Guo X, Okumura N, Nagai K, Hashimoto H, Baba A. PACAP-deficient mice exhibit light parameter-dependent abnormalities on nonvisual photoreception and early activity onset. PLoS One. Feb 18;5(2):e9286 (2010)

Unno, H., Yamashita, T., Ujita, S., Okumura, N., Otani, H., Okumura, A., Nagai, K. and Kusunoki, M. (2008). Structural basis for substrate recognition and hydrolysis by mouse carnosinase CN2. J Biol Chem 283, 27289-99.

Otani, H., Okumura, A., Nagai, K. and Okumura, N. (2008). Colocalization of a carnosine-splitting enzyme, tissue carnosinase (CN2)/cytosolic non-specific dipeptidase 2 (CNDP2), with histidine decarboxylase in the tuberomammillary nucleus of the hypothalamus. Neurosci Lett 445, 166-9.

Okumura, A., Nagai, K. and Okumura, N. (2008). Interaction of alpha1-syntrophin with multiple isoforms of heterotrimeric G protein alpha subunits. FEBS J 275, 22-33.

Gotoh, H., Okumura, A., Nagai, K. and Okumura, N. (2008). Localization of phospho-tyrosine489-beta-adducin immunoreactivity in the hypothalamic tanycytes and its involvement in energy homeostasis. Brain Res 1228, 97-106.

Tanida, M., Gotoh, H., Taniguchi, H., Otani, H., Shen, J., Nakamura, T., Tsuruoka, N., Kiso, Y., Okumura, N. and Nagai, K. (2007). Effects of central injection of L-carnosine on sympathetic nerve activity innervating brown adipose tissue and body temperature in rats. Regul Pept 144, 62-71.

Yamashita, T., Unno, H., Ujita, S., Otani, H., Okumura, N., Hashida-Okumura, A., Nagai, K. and Kusunoki, M. (2006). Crystallization and preliminary crystallographic study of carnosinase CN2 from mice. Acta Crystallogr Sect F Struct Biol Cryst Commun 62, 996-8.

Yamano, T., Tanida, M., Niijima, A., Maeda, K., Okumura, N., Fukushima, Y. and Nagai, K. (2006). Effects of the probiotic strain Lactobacillus johnsonii strain La1 on autonomic nerves and blood glucose in rats. Life Sci

Taniguchi, H., Tanida, M., Okumura, N., Hamada, J., Sano, S. and Nagai, K. (2006). Regulation of sympathetic and parasympathetic nerve activities by BIT/SHPS-1. Neurosci Lett 398, 102-6.

Kita, K., Okumura, N., Takao, T., Watanabe, M., Matsubara, T., Nishimura, O. and Nagai, K. (2006). Evidence for phosphorylation of rat liver glucose-regulated protein 58, GRP58/ERp57/ER-60, induced by fasting and leptin. FEBS Lett 580, 199-205.

Gotoh, H., Okumura, N., Yagi, T., Okumura, A., Shima, T. and Nagai, K. (2006). Fyn-induced phosphorylation of beta-adducin at tyrosine 489 and its role in their subcellular localization. Biochem Biophys Res Commun 346, 600-5.

Tanida, M., Yamano, T., Maeda, K., Okumura, N., Fukushima, Y. and Nagai, K. (2005). Effects of intraduodenal injection of Lactobacillus johnsonii La1 on renal sympathetic nerve activity and blood pressure in urethane-anesthetized rats. Neurosci Lett 389, 109-14.

Otani, H., Okumura, N., Hashida Okumura, A. and Nagai, K. (2005). Identification and characterization of a mouse dipeptidase that hydrolyzes L-carnosine. J Biochem (Tokyo) 137, 167-75.

Okumura, N., Hashida-Okumura, A., Kita, K., Matsubae, M., Matsubara, T., Takao, T. and Nagai, K. (2005). Proteomic analysis of slow- and fast-twitch skeletal muscles. Proteomics 5, 2896-906.

Matsubara, T., Okumura, N., Okumura, A. and Nagai, K. (2005). cGMP-dependent phosphorylation and degradation of myristoylated alanine-rich C-kinase substrate. Biochem Biophys Res Commun 326, 735-43.

Taniguchi, H., Okumura, N., Hamada, J., Inagaki, M., Nakahata, Y., Sano, S. and Nagai, K. (2004). Cold exposure induces tyrosine phosphorylation of BIT through NMDA receptors in the rat hypothalamus. Biochem Biophys Res Commun 319, 178-84.

Hamada, J., Okumura, N., Inagaki, M., Taniguchi, H., Nakahata, Y., Sano, S. and Nagai, K. (2004). Tyrosine phosphorylation of BIT on photic stimulation in the rat retina. FEBS Lett 557, 204-8.

Nakahata, Y., Okumura, N., Otani, H., Hamada, J., Numakawa, T., Sano, S. and Nagai, K. (2003). Stimulation of BIT induces a circadian phase shift of locomotor activity in rats. Brain Res 976, 194-201.

Nagai, K., Niijima, A., Yamano, T., Otani, H., Okumura, N., Tsuruoka, N., Nakai, M. and Kiso, Y. (2003). Possible role of L-carnosine in the regulation of blood glucose through controlling autonomic nerves. Exp. Biol. Med. 228, 1138-1145.

Isojima, Y., Okumura, N. and Nagai, K. (2003). Molecular mechanism of mammalian circadian clock. J Biochem 134, 777-84.

Shima, T., Okumura, N., Takao, T., Satomi, Y., Yagi, T., Okada, M. and Nagai, K. (2001). Interaction of the SH2 domain of Fyn with a cytoskeletal protein, beta-adducin. J Biol Chem 276, 42233-40.

Shima, T., Yagi, T., Isojima, Y., Okumura, N., Okada, M. and Nagai, K. (2000). Changes in circadian period and morphology of the hypothalamic suprachiasmatic nucleus in fyn kinase-deficient mice. Brain Res 870, 36-43.

Nakahata, Y., Okumura, N., Shima, T., Okada, M. and Nagai, K. (2000). Light-induced tyrosine phosphorylation of BIT in the rat suprachiasmatic nucleus. J Neurochem 74, 2436-44.

Hirose, T., Kawabuchi, M., Tamaru, T., Okumura, N., Nagai, K. and Okada, M. (2000). Identification of tudor repeat associator with PCTAIRE 2 (Trap). A novel protein that interacts with the N-terminal domain of PCTAIRE 2 in rat brain. Eur J Biochem 267, 2113-21.

Hidaka, Y., Shimono, C., Ohno, M., Okumura, N., Adermann, K., Forssmann, W.G. and Shimonishi, Y. (2000). Dual function of the propeptide of prouroguanylin in the folding of the mature peptide: disulfide-coupled folding and dimerization. J Biol Chem 275, 25155-62.

Fernandez de Cossio, J., Gonzalez, J., Satomi, Y., Shima, T., Okumura, N., Besada, V., Betancourt, L., Padron, G., Shimonishi, Y. and Takao, T. (2000). Automated interpretation of low-energy collision-induced dissociation spectra by SeqMS, a software aid for de novo sequencing by tandem mass spectrometry. Electrophoresis 21, 1694-9.

Hashida-Okumura, A., Okumura, N., Iwamatsu, A., Buijs, R.M., Romijn, H.J. and Nagai, K. (1999). Interaction of neuronal nitric-oxide synthase with alpha1-syntrophin in rat brain. J Biol Chem 274, 11736-41.

Kobayashi, S., Okumura, N., Okada, M. and Nagai, K. (1998). Depolarization-induced tyrosine phosphorylation of p130(cas). J Biochem (Tokyo) 123, 624-9.

Kobayashi, S., Okumura, N., Nakamoto, T., Okada, M., Hirai, H. and Nagai, K. (1997). Activation of pp60c-src depending on cell density in PC12h cells. J Biol Chem 272, 16262-7.

Hirose, T., Tamaru, T., Okumura, N., Nagai, K. and Okada, M. (1997). PCTAIRE 2, a Cdc2-related serine/threonine kinase, is predominantly expressed in terminally differentiated neurons. Eur J Biochem 249, 481-8.

Matsumine, A., Ogai, A., Senda, T., Okumura, N., Satoh, K., Baeg, G.H., Kawahara, T., Kobayashi, S., Okada, M., Toyoshima, K. and Akiyama, T. (1996). Binding of APC to the human homolog of the Drosophila discs large tumor suppressor protein. Science 272, 1020-3.

Khan, M.A., Okumura, N. and Okada, M. (1996). Depolarization-induced tyrosine phosphorylation of paxillin in PC12h cells. Eur J Biochem 235, 579-84.

Khan, M.A., Okumura, N., Okada, M., Kobayashi, S. and Nakagawa, H. (1995). Nerve growth factor stimulates tyrosine phosphorylation of paxillin in PC12h cells. FEBS Lett 362, 201-4.

Hashida-Okumura, A., Nagai, K., Okumura, N. and Nakagawa, H. (1995). Activation of neuronal nitric oxide synthase by flavin adenine dinucleotide. Biochem Mol Biol Int 35, 1339-48.

Okumura, N., Okada, M. and Nakagawa, H. (1994). Depolarization-induced tyrosine phosphorylation in PC12h cells. J Biochem (Tokyo) 116, 346-50.

Okumura, N., Okada, M., Nagai, K. and Nakagawa, H. (1994). Vasoactive intestinal peptide induces tyrosine phosphorylation in PC12h cells. J Biochem (Tokyo) 116, 341-5.

Okumura, N., Miyatake, Y., Takao, T., Tamaru, T., Nagai, K., Okada, M. and Nakagawa, H. (1994). Vasoactive intestinal peptide induces differentiation and MAP kinase activation in PC12h cells. J Biochem (Tokyo) 115, 304-8.

Nagai, K., Nagai, N., Isojima, Y., Itoh, S., Okumura, N. and Nakagawa, H. (1994). Mazindol enhances glucose uptake by rat skeletal muscle. Eur J Pharmacol 260, 29-37.

Matsuda, S., Ii, Y., Desaki, J., Yoshimura, H., Okumura, N. and Sakanaka, M. (1994). Development of Purkinje cell bodies and processes with basic fibroblast growth factor-like immunoreactivity in the rat cerebellum. Neuroscience 59, 651-62.

Okada, K., Matsuda, S., Ii, Y., Okumura, N., Uryu, K., Fujita, H. and Sakanaka, M. (1993). Basic fibroblast growth factor-like immunoreactivity in the rat trigeminal sensory system and peri-oral skin with vibrissae. Cell Tissue Res 272, 417-27.

Morimoto, A., Matsuda, S., Uryu, K., Fujita, H., Okumura, N. and Sakanaka, M. (1993). Light- and electron-microscopic localization of basic fibroblast growth factor in adult rat retina. Okajimas Folia Anat Jpn 70, 7-12.

Miyatake, Y., Okumura, N., Nagai, K. and Nakagawa, H. (1993). The signal transduction pathway for VGF expression due to NGF is different from that due to bFGF in PC12h cell. Biochem Mol Biol Int 30, 231-6.

Matsuda, S., Fujita, H., Imaoka, S., Okumura, N. and Sakanaka, M. (1993). Immunoelectron microscopic localization of basic FGF in neuroglias and neurons of the trigeminal mesencephalic and motor nuclei. Okajimas Folia Anat Jpn 69, 335-43.

Imaizumi, K., Iwata, H., Yoshida, S., Sun, G., Okumura, N. and Shiosaka, S. (1993). Coexistence of amyloid beta-protein precursor and basic fibroblast growth factor in single cells of the rat parietal cortex, hippocampus and basal magnocellular nucleus. J Chem Neuroanat 6, 159-65.

Funakoshi, Y., Matsuda, S., Uryu, K., Fujita, H., Okumura, N. and Sakanaka, M. (1993). An immunohistochemical study of basic fibroblast growth factor in the developing chick. Anat Embryol (Berl) 187, 415-23.

Okumura, N. and Shimazu, T. (1992). Vanadate stimulates D-glucose transport into sarcolemmal vesicles from rat skeletal muscles. J Biochem (Tokyo) 112, 107-11.

Matsuyama, A., Iwata, H., Okumura, N., Yoshida, S., Imaizumi, K., Lee, Y., Shiraishi, S. and Shiosaka, S. (1992). Localization of basic fibroblast growth factor-like immunoreactivity in the rat brain. Brain Res 587, 49-65.

Matsuda, S., Okumura, N., Yoshimura, H., Koyama, Y. and Sakanaka, M. (1992). Basic fibroblast growth factor-like immunoreactivity in Purkinje cells of the rat cerebellum. Neuroscience 50, 99-106.

Matsuda, S., Desaki, J., Okumura, N., Shiosaka, S., Imaoka, S. and Sakanaka, M. (1992). Basic fibroblast growth factor-like immunoreactivity in the trigeminal proprioceptive and motor systems. Brain Res 577, 92-100.

Matsuda, S., Desaki, J., Fujita, H., Okumura, N. and Sakanaka, M. (1992). Immuno-electron-microscopic localization of basic fibroblast growth factor in the dystrophic mdx mouse masseter muscle. Cell Tissue Res 270, 569-76.

Desaki, J., Matsuda, S., Okumura, N., Koyama, Y. and Sakanaka, M. (1992). Fine structure of nerve processes containing basic fibroblast growth factor in muscle spindles of the rat masseter muscle. Neurosci Lett 137, 237-40.

Iwata, H., Matsuyama, A., Okumura, N., Yoshida, S., Lee, Y., Imaizumi, K. and Shiosaka, S. (1991). Localization of basic FGF-like immunoreactivity in the hypothalamo-hypophyseal neuroendocrine axis. Brain Res 550, 329-32.

Okumura, N., Takimoto, K., Okada, M. and Nakagawa, H. (1989). C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. J Biochem (Tokyo) 106, 904-9.

Hara, M., Matsuda, Y., Okumura, N., Hirai, K. and Nakagawa, H. (1989). Effect of glucose starvation on glucose transport in neuronal cells in primary culture from rat brain. J Neurochem 52, 909-12.

Hara, M., Matsuda, Y., Hirai, K., Okumura, N. and Nakagawa, H. (1989). Characteristics of glucose transport in neuronal cells and astrocytes from rat brain in primary culture. J Neurochem 52, 902-8.

著書(分担執筆)

Okumura N, Tanida M, Niijima A, Tsuruoka N, Kiso Y, Horii Y, Nagai K. Carnosine and the control of blood glucose. in "Imidazole dipeptides - chemistry, analysis, function and effects", pp. 257-276, Royal Society of Chemistry (2015).

Okumura N. Carnosine dipeptidase II. in Handbook of Proteolytic Enzymes, pp. 1597-1600, (2012) Academic Press.

奥村宣明 「蛋白質の定量法」 蛋白質実験ノート改訂第4版(上) pp 29-34、羊土社 (2011).

奥村宣明 「ポリアクリアルアミドゲル電気泳動・ウエスタンブロッティング」蛋白質実験ノート改訂第4版(下) pp 17-55、羊土社 (2011).

奥村宣明 「プロテオミクスの基礎技術」組織細胞化学2005 pp17-22 (2005).

奥村宣明、永井克也 「二次元ゲル電気泳動」、 ゲノミクス・プロテオミクスの新展開 エヌ・ティー・エス社 pp468-473 (2004).

奥村宣明 「蛋白質の定量法」 蛋白質実験ノート改訂第3版(上) pp 29-34、羊土社 (2004).

奥村宣明 「ポリアクリアルアミドゲル電気泳動・ウエスタンブロッティング」蛋白質実験ノート改訂第3版(下) pp 17-47、羊土社 (2004). 

Reviews

Nagai K, Tanida M, Niijima A, Tsuruoka N, Kiso Y, Horii Y, Shen J, Okumura N.Role of L-carnosine in the control of blood glucose, blood pressure, thermogenesis, and lipolysis by autonomic nerves in rats: involvement of the circadian clock and histamine.Amino Acids. 2012 Jul;43(1):97-109.

奥村宣明、須藤浩三、高尾敏文 「尿のペプチドミクス」日本腎臓学会誌 52, 485-488 (2010)

Nakagawa H, Okumura N. Coordinated regulation of circadian rhythms and homeostasis by the suprachiasmatic nucleus. Proc Jpn Acad Ser B Phys Biol Sci. 86, 391-409 (2010)

永井克也、谷田守、福島洋一、山野俊彦、新島旭、前田景子、奥村宣明、堀井裕子、沈嬌、 「乳酸菌の腸内投与による自律神経活動と生理機能の変化」」 腸内細菌学雑誌 23、209-216 (2009)

谷田守、山野俊彦、前田景子、福島洋一、奥村宣明、永井克也、「LC1乳酸菌の自律神経を介する血圧低下作用」 肥満研究 12, 247-250(2006)

奥村宣明、谷口裕幸、濱田樹理、中畑泰和、奥村明子、永井克也「寒冷曝露によってチロシンリン酸化の亢進するタンパク質の解析−BIT/SHPS-1と自律神経」 肥満研究 11:328-331 (2005)

Isojima, Y., Okumura, N., and Nagai, K. (2003) Molecular mechanism of mammalian circadian clock. J Biochem (Tokyo) 134, 777-784

奥村宣明、奥村明子、永井克也 「自律神経系による代謝調節」 日本臨床別冊、肥満症 61:259-264 (2003)

永井克也、礒島康史、奥村宣明 「哺乳類の概日リズムの時刻発信機構」蛋白質・核酸・酵素 47:1914-1923 (2002)

奥村宣明、永井克也 「プロテオミクスにおける二次元電気泳動」 Molecular Medicine 別冊、癌ゲノム学、39:158-165 (2002)

高尾敏文、松八重雅美、奥村宣明、永井克也 「プロテオーム:分析法」蛋白質・核酸・酵素 46:1504-1509 (2001)

原著論文

著書(分担執筆)

総説