【Paper】

* corresponding author, † equal contribution

2019

■Multifunctional temozolomide-loaded lipid superparamagnetic nanovectors: Dual targeting and disintegration of glioblastoma spheroids by synergic chemotherapy and hyperthermia treatment.
Attilio Marino, Alice Camponovo, Andrea degl’Innocenti, Martina Bartolucci, Christos Tapeinos, Chiara Martinelli, Daniele de Pasquale, Francesca Santoro, Valentina Mollo, Satoshi Arai, Madoka Suzuki, Yoshie Harada, Andrea Petretto and Gianni Ciofani
Nanoscale, in press
doi: 10.1039/C9NR07976A. (link)

■Polydopamine Coating as a Scaffold for Ring-Opening Chemistry To Functionalize Gold Nanoparticles.
*Shingo Sotoma, Yoshie Harada
Langmuir. 35, 8357-8362 (2019).
doi: 10.1021/acs.langmuir.9b00762. (link)

■HCV IRES captures an actively translating 80S ribosome.
Takeshi Yokoyama, Kodai Machida†, Wakana Iwasaki†, Tomoaki Shigeta, Madoka Nishimoto, Mari Takahashi, Ayako Sakamoto, Mayumi Yonemochi, Yoshie Harada, Hideki Shigematsu, Mikako Shirouzu, *Hisashi Tadakuma, *Hiroaki Imataka, *Takuhiro Ito
Molecular Cell. 74(6), 1205-1214 (2019).
doi: 10.1016/j.molcel.2019.04.022. (link)

2018

■Magnetic Field Imaging of Superparamagnetic Particles Using High-Density, Perfectly Oriented NV Centers in Diamond CVD Film
*Yuji Hatano, Takeharu Sekiguchi, Takayuki Iwasaki, Mutsuko Hatano, and Yoshie Harada
Physica Status Solidi (a), 215(22), pssa.201800254 (2018).
doi: 10.1002/pssa.201800254 (link).

■Fluorescent nanodiamonds as a robust temperature sensor inside a single cell.
Takeharu Sekiguchi, Shingo Sotoma†, *Yoshie Harada
Biophysics and Physicobiology, 15, 229-234 (2018),
doi: 10.2142/biophysico.15.0_229 (link).

■Construction of integrated gene logic-chip.
Takeya Masubuchi, *Masayuki Endo, Ryo Iizuka, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Hao Qi, Ryosuke Iinuma, Yuya Miyazono, Shuichi Shoji, Takashi Funatsu, *Hiroshi Sugiyama, Yoshie Harada, *Takuya Ueda, *Hisashi Tadakuma
Nature Nanotechnology. 13(10), 933-940 (2018).
doi: 10.1038/s41565-018-0202-3. (link)

■Enrichment of ODMR-active nitrogen-vacancy centres in five-nanometre-sized detonation-synthesized nanodiamonds: Nanoprobes for temperature, angle and position.
Shingo Sotoma, Daiki Terada, Takuya F. Segawa, *Ryuji Igarashi, *Yoshie Harada, *Masahiro Shirakawa
Scientific Reports, 8(1), pp 5463 (2018),
doi:10.1038/s41598-018-23635-5 (link).

■Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas.
Sotaro Fujii, Misa Masanari-Fujii, Shinya Kobayashi, Chiaki Kato, Masayoshi Nishiyama, Yoshie Harada, Satoshi Wakai, *Yoshihiro Sambongi
Biosci Biotechnol Biochem., 82(5), pp 792-799 (2018),
doi:10.1080/09168451.2018.1448255 (link).

■Morphological Control of Microtubule-Encapsulating Giant Vesicles by Changing Hydrostatic Pressure.
*Kingo Takiguchi, Masahito Hayashi, Yuki Kazayama, Taro Toyota, Yoshie Harada, Masayoshi Nishiyama
Biol Pharm Bull., 41(3), pp 288-293 (2018),
doi:10.1248/bpb.b17-00366 (link).

2017

■Live Cell Imaging Using Photoswitchable Diarylethene-Doped Fluorescent Polymer Dots.
*Yasuko Osakada, *Tuyoshi Fukaminato, Yuma Ichinose, *Mamoru Fujitsuka, Yoshie Harada, *Tetsuro Majima
Chem Asian J., 12(20), pp 2660-2665 (2017),
doi:10.1002/asia.201701038 (link).

2016

■Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein.
Seiichiro Kizaki, Tingting Zou, Yue Li, Yong‐Woon Han, Yuki Suzuki, Yoshie Harada, *Hiroshi Sugiyama
Chemistry, 22(46), pp 16598-16601 (2016),
doi:10.1002/chem.201602435 (link).

■Reversible Morphological Control of Tubulin-Encapsulating Giant Liposomes by Hydrostatic Pressure.
Masahito Hayashi, *Masayoshi Nishiyama, Yuki Kazayama, Taro Toyota, Yoshie Harada, and *Kingo Takiguchi
Langmuir, 32 (15), pp 3794–3802 (2016), doi:10.1021/acs.langmuir.6b00799 (link).

■The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein–DNA complex formation.
*Yong-Woon Han, *Hiroshi Sugiyama and *Yoshie Harada
Biomater. Sci, 4(3), pp 391-399 (2016), doi:10.1039/C5BM00214A (link).

2015

■Synergistic effect of ATP for RuvA-RuvB-Holiday junction DNA complex formation.
*Takuma Iwasa, *Yong-Woon Han, Ryo Hiramatsu, †Hiroaki Yokota, Kimiko Nakao, Ryuji Yokokawa, Teruo Ono and *Yoshie Harad
Scientific Reports, 5, pp 18177 (2015) doi:10.1038/srep18177 (link).

■Suppression of Nonspecific Protein–Nanodiamond Adsorption Enabling Specific Targeting of Nanodiamonds to Biomolecules of Interest
Shingo Sotoma, Ryuji Igarashi*, Jun Iimura, Yuta Kumiya, Hidehito Tochio, Yoshie Harada and Masahiro Shirakawa*
Chemistry Letters, 44(3), pp 354-356 (2015) doi:org/10.1246/cl.141036 (link).

■ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues Nucl.
Ikumi Oomoto, Asuka Suzuki-Hirano, Hiroki Umeshima, Yong-Woon Han, Hiroyuki Yanagisawa, Peter Carlton, Yoshie Harada, Mineko Kengaku, Akimitsu Okamoto, Tomomi Shimogori and *Dan Ohtan Wang
Nucleic Acids Res., 43(19), e126 (2015), doi:10.1093/nar/gkv614 (link).

■Comprehensive and quantitative analysis for controlling the physical/chemical states and particle properties of nanodiamonds
for biological applications.
Shingo Sotoma, Kenichi Akagi, Saburo Hosokawa, Ryuji Igarashi, Hideto Tochio, Yoshie Harada and *Masahiro Shirakawa
RSC Adv., 5, pp 13818-13827 (2015), doi: 10.1039/C4RA16482B (link).

■Optically Detected Magnetic Resonance of Nanodiamonds In Vivo; Implementation of Selective Imaging and Fast Sampling.
*Yosuke Yoshinari, Shigeyuki Mori, Ryuji Igarashi, Takuma Sugi, Hiroaki Yokota, Kazuhiro Ikeda, Hitoshi Sumiya, Ikue Mori, Hidehito Tochio, *Yoshie Harada and Masahiro Shirakawa
Journal of Nanoscience and Nanotechnology, 15, pp 1014-1021(2015), doi:10.1166/jnn.2015.9739 (link).

2014

■Quantitative in vivo FCCS analyses highlight the importance of competitive effects in the regulation of protein-protein interactions.
Wakako Sadaie, Yoshie Harada, Michiyuki Matsuda and *Kazuhiro Aoki
Molecular and Cellular Biology, 34(17), pp 3272-3290 (2014), doi:10.1128/MCB.00087-14 (link).

■Effective Production of Fluorescent Nanodiamond Containing Negatively-Charged Nitrogen-Vacancy Centers by Ion Irradiation.
Shingo Sotoma, Yosuke Yoshinari, Ryuji Igarashi, Akiyoshi Yamazaki, Shige H. Yoshimura, Hidesato Tochio, Masahiro Shirakawa and *Yoshie Harada
Diamond and Related Materials, 49, pp 33-38 (2014), doi:10.1016/j.diamond.2014.07.011 (pdf).

■Real-time single-cell imaging of protein secretion.
Yoshitaka Shirasaki, Mai Yamagishi, Nobutake Suzuki, Kazushi Izawa, Asahi Nakahara, Jun Mizuno, Shuichi Shoji, Toshio Heike, Yoshie Harada, Ryuta Nishikomori and *Osamu Ohara
Scientific Reports, 4, pp 4736 (2014), doi:10.1038/srep04736 (link).

■Hard X-ray-induced optical luminescence via　biomolecule-directed metal clusters.
*Yasuko Osakada, Guillem Pratx, Conroy Sun, Masanori Sakamoto, Moiz Ahmad, Olga Volotskova, Qunxiang Ong, Toshiharu Teranishi, Yoshie Harada, *Lei Xing and *Bianxiao Cui
Chemical Communications (Camb), 50, pp 3549-3551 (2014), doi:10.1039/c3cc48661c (link).

■Construction and Characterization of Cy3- or Cy5-Conjugated Hairpin Pyrrole/Imidazole Polyamides binding to DNA in the Nucleosome.
*Yong-Woon Han, Yasuo Tsunaka, Hiroaki Yokota, Tomoko Matsumoto, Gengo Kashiwazaki, Hironobu Morinaga, Kaori Hashiya, Toshikazu Bando,
*Hiroshi Sugiyama and *Yoshie Harada
Biomaterials Science, 2, pp 297-307 (2014), doi:10.1039/C3BM60202H (link).

2013

■Observing the rotational diffusion of nanodiamonds with arbitrary nitrogen vacancy center configurations.
*Yohsuke Yoshinari, *Ziya Kalay and *Yoshie Harada
Phys. Rev. B, 88, pp 235206 (2013), doi:10.1103/PhysRevB.88.235206 (link).

■In Vivo Fluorescent Adenosine 5′-Triphosphate (ATP) Imaging of　Drosophila melanogaster and Caenorhabditis elegans
by Using a Genetically Encoded Fluorescent ATP Biosensor Optimized for Low Temperatures.
†Taiichi Tsuyama, †Jun-ichi Kishikawa, Yong-Woon Han, Yoshie Harada, Asako Tsubouchi, Hiroyuki Noji, Akira Kakizuka, Ken Yokoyama, Tadashi Uemura and †Hiromi Imamura
dx.doi.org/10.1021/ac4015325 Anal. Chem., 85, pp 7889－7896 (2013), doi:10.1021/ac4015325 (link).

■Effect of single Pyrrole replacement with balanine on DNA binding affinity and sequence specificity of
hairpin Pyrrole/Imidazole Polyamides targeting 5´-GCGC-3´.
*Yong-Woon Han, Gengo Kashiwazaki, Hironobu Morinaga, Tomoko Matsumoto, Kaori Hashiya, Toshikazu Bando, *Yoshie Harada and *Hiroshi Sugiyama
Bioorganic & Medicinal Chemistry,　21(17), pp 5436–5441 (2013), doi:10.1016/j.bmc.2013.06.005 (link).

■Single-Molecule Imaging of the Oligomer Formation of the Nonhexameric Escherichiacoli UvrD Helicase.
*Hiroaki Yokota, Yuko Ayabe Chujo and Yoshie Harada
Biophysical Journal, 104, 924–933 (2013), doi:10.1016/j.bpj.2013.01.014 (link).

■ParA-mediated plasmid partition driven by protein pattern self-organization.
†Ling Chin Hwang, †Anthony G Vecchiarelli, Yong-Woon Han, Michiyo Mizuuchi, Yoshie Harada, Barbara E Funnell and *Kiyoshi Mizuuchi
The EMBO Journal, 32(9), pp 1238-1249 (2013), doi:10.1038/emboj.2013.34 (pdf).

2012

■Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy.
Kohki Okabe, Noriko Inada, Chie Gota, Yoshie Harada, Takashi Funatsu and *Seiichi Uchiyama
Nature Communications, 3, 705 (2012), doi:10.1038/ncomms1714 (link).

■Real-Time Background-Free Selective Imaging of Fluorescent Nanodiamonds in Vivo.
†Ryuji Igarashi, †Yousuke Yoshinari, †Hiroaki Yokota, Takuma Sugi, Fuminori Sugihara, Kazuhiro Ikeda, Hitoshi Sumiya, Shigenori Tsuji, Ikue Mori, Hidehito Tochio, *Yoshie Harada and *Masahiro Shirakawa
Nano Letters, 12, 5726-5732 (2012), doi:10.1021/nl302979d (link).

■Binding of hairpin pyrrole and imidazole polymides to DNA:relationship between torsion angle and association rate constants.
*Yong-Woon Han, Tomoko Matsumoto, Hiroaki Yokota, Gengo Kashiwazaki, Hironobu Morinaga, Kaori Hashiya, Toshikazu Bando,*Yoshie Harada and *Hiroshi Sugiyama
Nucleic Acids Research, 40, pp 11510-11517 (2012), doi:10.1093/nar/gks897 (link).

■Direct Visualization of the Movement of a Single T7 RNA Polemerase and Transcription on a DNA Nanostructure.
*Masayuki Endo, Koichi Tatsumi, Kosuke Terushima , Yosuke Katsuda, Kumi Hidaka, Yoshie Harada, *Hiroshi Sugiyama
Angew.Chem.Int.Ed.Engl., 51(35), pp 8778-8782 (2012), DOI:10.1002/anie.201201890 (link).

■Direct observation stand passage by DNA-topoisomerase and its micrometre processivity.
Katsunori Yogo, Taisaku Ogawa, Masahito Hayashi, Yoshie Harada, Takayuki Nishizaka and *Kazuhiro Kinoshita Jr.
PLoS ONE., 7(4), e34920 (2012), doi:10.1371/journal.pone.0034920 (pdf).

2011

■Facilitated Intracellular Transport of TrkA by an Interaction with Nerve Growth Factor.
Mami Nomura, Takeharu Nagai, Yoshie Harada and *Tomomi Tani
Developmetal Neurobiology, 71(7), pp 634-649 (2011), doi:10.1002/dneu.20879 (link).

■Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2′ -O-methyl RNA probes in living cells
Kohki Okabe, Yoshie Harada, Junwei Zhang, Hisashi Tadakuma, Tokio Tani and *Takashi Funatsu
Nucleic Acids Research, 39(4), e20 (2011), doi:10.1093/nar/gkq1196 (link).

【Publications】

2010

■Strain through the neck linker ensures processive runs: a DNA-kinesin hybrid nanomachine study.
Yuya Miyazono, Masahito Hayashi, Peter Karagiannis, Yoshie Harada and *Hisashi Tadakuma
The EMBO Journal, 29, 93-106 (2010), doi:10.1038/emboj.2009.319 (pdf).

2009

■Single-molecule visualization of binding modes of helicase to DNA on PEGylated surface.
*Hiroaki Yokota, Yong-Woon Han, Jean-Francois Allemand, Xuguang Xi, David Bensimon, Vincent Croquette, Yoshihiro Ito and Yoshie Harada
Chemistry Letters, 38(4), 308-309 (2009), doi:10.1246/cl.2009.308 (pdf).

■Hydrophilic fluorescent nanogel thermometer for intracellular thermometry.
Chie Gota, Kohki Okabe, Takashi Funatsu, Yoshie Harada and *Seiichi Uchiyama
Journal of the American Chemical Society, 131(8), 2766-2767 (2009), doi:10.1021/ja807714j (link).

2008

■Single-cell chemical lysis method for analysis of intracellular molecules using an array of picoliter-scale microwells.
Yasuhiro Sasuga, Tomoyuki Iwasawa, Kayoko Terada, Yoshihiro Oe, Hiroyuki Sorimachi, *Osamu Ohara and Yoshie Harada
Analytical Chemistry , 80(23), 9141-9149 (2008), doi:10.1021/ac8016423 (link).

2007

■Direct observation of the reversible unwinding of a single DNA molecule caused by the intercalation of ethidium bromide.
*Masahito Hayashi and Yoshie Harada
Nucleic Acids Research, 35(19), e125 (2007), doi:10.1093/nar/qkm529 (link).

■Visualization of RecA filaments and DNA by fluorescence microscopy.
*Taro Nishinaka, Yuko Doi, Makiko Hashimoto, Reiko Hara, Takehiko Shibata, Yoshie Harada, Kazuhiko Kinosita Jr, Hiroyuki Noji and Eiji Yashima
J. Biochem., 141(2), 147-156 (2007), doi:10.1093/jb/mvm033 (pdf).

■Inhibition of Reversed Electron Transfer and Proton Transport in the Beef Heart Cytochrome bc1 Complex by Chemical Modification.
*Toshiaki Miki, Masato Umeda and Yoshie Harada
J. Biochem., 141(3), 377-387 (2007), doi:10.1093/jb/mvm042 (link).

2006

■Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex.
Yong-Woon Han, Tomomi Tani, Masahito Hayashi, Takashi Hishida, Hiroshi Iwasaki, Hideo Shinagawa and *Yoshie Harada
Proc. Natl. Acad. Sci. USA., 103(31), 11544-11548 (2006), doi:10.1073/pnas.0600753103 (link).

■Development of a microscopic platform for real-time monitoring of biomolecular interactions.
Yasuhiro Sasuga, Tomomi Tani, Masahito Hayashi, Hisashi Yamakawa, *Osamu Ohara and Yoshie Harada
Genome Res., 16, 132-139 (2006), doi:10.1101/gr.4235806 (link).

2005

■Structure-function analysis of the three domains of RuvB DNA motor protein.
Takayuki Ohnishi, Takashi Hishida, Yoshie Harada, Hiroshi Iwasaki and *Hideo Shinagawa
J. Biol. Chem., 280, 30504-30510 (2005), doi:10.1074/jbc.M502400200 (link).

■Trafficking of a ligand-receptor complex on the growth cones as an essential step for the uptake of nerve growth factor
at the distal end of axon: a single-molecule analysis
*Tomomi Tani, Yoshikazu Miyamoto, Kazuhiro E. Fujimori, Takahisa Taguchi, Toshio Yanagida, Yasushi Sako and Yoshie Harada
J. Neurosci., 25, 2181-2191 (2005), doi:10.1523/JNEUROSCI.4570-04.2005 (link).

【Publications】

2004

■Studies on biomolecules using single molecule imaging and manipulation techniques.
*Yoshie Harada
Sci. Tech. Adv. Mat.5, 709-713, doi: 10.1016/j.stam.2004.02.014 (link).

2001

■Direct observation of DNA rotation during transcription by Escherichia coli RNA polymerase.
*Yoshie Harada, Osamu Ohara, Akira Takatsuki, Hiroyasu Itoh, Nobuo Shimamoto and Kazuhiko Kinosita Jr
Nature409, 113-115, doi:10.1038/35051126 (link).

2000

■Stepping rotation of F1-ATPase visualized through angle-resolved single-fluorophore imaging.
Kengo Adachi, Ryohei Yasuda, Hiroyuki Noji, Hiroyasu Itoh, Yoshie Harada, Masasuke Yoshida and Kazuhiko Kinosita Jr
Proc. Natl. Acad. Sci. USA97, 7243-7247, doi: 10.1073/pnas.120174297 (pdf).

1999

■Tying a molecular knot with optical tweezers.
Yasuharu Arai, Ryohei Yasuda, Ken-ichirou Akashi, *Yoshie Harada, Hidetake Miyata, Kazuhiko Kinosita Jr. and Hiroyasu Itoh
Nature399, 446-448, doi:10.1038/20894 (link).

■Single molecule imaging of RNA polymerase-DNA interactions in real time.
*Yoshie Harada, Takashi Funatsu, Katsuhiko Murakami, Yoshikazu Nonoyama, Akira Ishihama and Toshio Yanagida
Biophys. J.76, 709-715, doi: 10.1016/S0006-3495(99)77237-1 (pdf).

1997

■Imaging and nano-manipulation of single bio-molecules.
*Takashi Funatsu, Yoshie Harada, Hideo Higuchi, Makio Tokunaga, Kiwamu Saito, Yoshiharu Ishii, Ron D. Vale and Toshio Yanagida
Biophys. Chem.68, 63-72, doi: 10.1016/S0301-4622(97)00008-2 (link).

■Single molecular assay of the individual ATP turnovers by a myosin-GFP fusion Protein expressed in vitro.
*Atsuko Hikikoshi Iwane, Takashi Funatsu, Yoshie Harada, Makio Tokunaga, Osamu Morimoto and Toshio Yanagida
FEBS Letters407, 235-238 (link).

■Coupling of protein surface hydrophobicity change to ATP hydrolysis by myosin motor domain.
*Makoto Suzuki, Junji Shigematsu, Yoshifumi Fukunishi, Yoshie Harada, Toshio Yanagida and Takao Kodama
Biophys. J.72, 18-23, doi: 10.1016/S0006-3495(97)78643-0 (pdf).

1996

■Direct inhibition of the actomyosin motility by local anesthetics in vitro.
*Yuri Tsuda, Takashi Mashimo, Ikuto Yoshiya, Kuniyoshi Kaseda, Yoshie Harada and Toshio Yanagida
Biophys. J.71, 2733-2741, doi: 10.1016/S0006-3495(96)79466-3 (link).

■Direct observation of single kinesin molecules moving along microtubules.
*Ron D. Vale, Takashi Funatsu, Laura Romberg, Dan W. Pierce, Yoshie Harada and Toshio Yanagida
Nature380, 451-453, doi: 10.1038/380451a0 (pdf).

■Multiple and single-molecule analysis of the actomyosin motor by nanometer-piconewton manipulation with a microneedle:
Unitaly steps and forces.
*Akihiko Ishijima, Hiroaki Kojima, Hideo Higuchi, Yoshie Harada, Takashi Funatsu and Toshio Yanagida
Biophys. J.70, 383-400, doi: 10.1016/S0006-3495(96)79582-6 (pdf).

1995

■Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution.
Takashi Funatsu, Yoshie Harada, Makio Tokunaga, Kiwamu Saito and *Toshio Yanagida
Nature374, 555-559, doi:10.1038/374555a0 (link).

【Publications】

1994

■Single-molecule analysis of the actomyosin motor using nano-manipulation.
Akihiko Ishijima, Yoshie Harada, Hiroaki Kojima, Takashi Funatsu, Hideo Higuchi and Toshio Yanagida
Biochem. Biophys. Res. Commun.199, 1057-1063, doi: 10.1006/bbrc.1994.1336 (link).

1993

■Force-generating domain of myosin motor.
Shinji Itakura, Hisashi Yamakawa, Yoko Yano Toyoshima, Akihiko Ishijima, Hiroaki Kojima, Yoshie Harada, Toshio Yanagida, Takeyuki Wakabayashi and *Kazuo Sutoh
Biochem. Biophys. Res. Commun.196, 1504-1510 (link).

1990

■Mechanochemical coupling in actomyosin energy transduction studied by in vitro. movement assay.
Yoshie Harada, Katsuhiko Sakurada, Toshiaki Aoki, David D. Thomas and Toshio Yanagida
J. Mol. Biol.216, 49-68 (link).

1988

■Direct observation of molecular motility by light microscopy.
Yoshie Harada and Toshio Yanagida
Cell Motility and the Cytoskeleton10, 71-76 (link).

1987

■Sliding movement of single actin filaments on one-headed myosin filaments.
Yoshie Harada, Akira Noguchi, Akiyoshi Kishino and Toshio Yanagida
Nature326, 805-808 (link).

【Reviews】

2014

■Optically detected magnetic resonance of nanodiamonds in vivo.
Yoshie Harada
Lecture Notes on Cell Biology and Materials Science, Yuh-Jyh Jong and Norio Nakatsuji Editors University of Taiwan System Press (link).

1998

■Single molecule imaging and nanomanipulation of biomolecules.
Yoshie Harada, Takashi Funatsu, Makio Tokunaga, Kiwamu Saito, Hideo Higuchi, Yoshiharu Ishii and Toshio Yanagida
Single molecule imaging and nanomanipulation of biomolecules, Methods Cell Biol. 55, 117-128 (link).

1993

■Nano-manipulation of actomyosin molecular motors in vitro: a new working principle.
Toshio Yanagida, Yoshie Harada and Akihiko Ishijima
Trends in Biochemical Sciences18, 319-324 (link).

■Coupling between ATPase and force-generating attachment-detachment cycles of actomyosin in vitro.
Toshio Yanagida, Akihiko Ishijima, Kiwamu Saito and Yoshie Harada
Mechanism of Myofilament Sliding in Muscle Contraction, Edited by H. Sugi and G. H. Pollack
Plenum Press, New York339-349 (link).

■Do the two heads of the myosin molecule function independently or cooperatively?
Yoshie Harada and Toshio Yanagida
Molecular and Cellular Biology, Edited by S. L. Wolfe, Wadswarth 464-465

1991

■Chemomechanical coupling in actomyosin system: An approach by in vitro movement assay and kinetic analysis of ATP hydrolysis by shortening myofibrils.
Toshio Yanagida, Yoshie Harada and Takao Kodama
Advances in Biophysics27, 237-257

1989

■The elementary process in the actomyosin energy transduction system.
Yoshie Harada and Toshio Yanagida
Muscle Energetics (R. J. Paul, G. Elzinga and K. Yamada eds.) Alan R. Liss, Inc.27-36 (link).

1988

■Minimum structural unit required for energy transduction in muscle.
Toshio Yanagida, Yoshie Harada
Molecular Mechanism of Muscle Contraction (Sugi, H. & Pollack, G. H. eds.) Plenum New York11-19 (link).