(for reprint request, send e-mail to J. Takagi)

[2025]

• Engineered ACE2 Decoy in Dry Powder Form for Inhalation: A Novel Therapy for SARS-CoV-2 Variants
• Ito T, Suzuki T, Sakai Y, Nishioka K, Itoh Y, Sakamoto K, Ikemura N, Matoba S, Kanda Y, Takagi J, Okamoto T, Tahara K, Hoshino A.
  Ther. Methods & Clin. Dev. (2025) 33(2):101459 [Pubmed]
• Immunological analysis of LC16m8 vaccine: preclinical and early clinical insights into mpox
• Kobiyama K, Utsumi D, Kaku Y, Sasaki E, Yasui F, Okamura T, Onodera T, Tobuse AJ, Sakkour A, Amiry AF, Hayashi T, Temizoz B, Liu K, Negishi H, Toyama-Sorimachi N, Kohara M, Sawasaki T, Takagi J, Sato K, Takahashi Y, Yasutomi Y, Ishii KJ.
  eBioMedicine (2025) 115:105703 [Pubmed]
• CAR T or NK cells targeting mismatched HLA-DR molecules in acute myeloid leukemia after allogeneic hematopoietic stem cell transplant
• Ikeda S, Hasegawa K, Kogue Y, Arimori T, Kawamoto R, Wibowo T, Yaga M, Inada Y, Uehara H, Matsubara M, Tachikawa M, Suga M, Kida S, Shibata K, Tsutsumi K, Fukushima K, Fujita J, Ueda T, Kusakabe S, Hino A, Ichii M, Hirose A, Nakamae H, Hino M, Nakao T, Inoue M, Yoshihara K, Yoshihara S, Ueda S, Tachi T, Kuroda H, Murakami K, Kijima N, Kishima H, Igashira E, Murakami M, Takiuchi T, Kimura T, Hiroshima T, Kimura T, Shintani Y, Imai C, Yusa K, Mori R, Ogino T, Eguchi H, Takeda K, Oji Y, Kumanogoh A, Takagi J, Hosen N.
  Nature Cancer (2025) 6(4):595-611 [Pubmed]
• Impact of mechanotransduction on gene expression changes in periodontal ligament during orthodontic tooth movement
• Chida S, Chiba T, Uchida Y, Matsushima T, Kurimoto R, Miyazaki T, Yagasaki L, Nakamura S, Mihara E, Takagi J, Moriyama K, Asahara H.
  J. Bone Miner. Metab. (2025) in press [Pubmed]
[2024]

• Development of a new antibody drug to treat congenital tooth agenesis
• Takahashi K, Kiso H, Mihara E, Takagi J, Tokita Y, Murashima-Suginami A.
  J. Oral Biosci. (2024) 66(4):1-9 [Pubmed]
• A cyclic peptide-grafted Fc with hepatocyte growth factor functionality ameliorates hepatic fibrosis in a non-alcoholic steatohepatitis mouse model
• Rojas-Chaverra NM, Imamura R, Sato H, Passioura T, Mihara E, Nishimura T, Takagi J, Suga H, Matsumoto K, Sakai K.
  iScience (2024) 27(8):110426 [Pubmed]
• PlexinB1 Inactivation Reprograms Immune Cells in the Tumor Microenvironment, Inhibiting Breast Cancer Growth and Metastatic Dissemination
• Franzolin G, Brundu S, Cojocaru CF, Curatolo A, Ponzo M, Mastrantonio R, Mihara E, Kumanogoh A, Suga H, Takagi J, Tamagnone L, Giraudo E.
  Cancer Immunol Res. (2024) 12(9):1286-1301 [Pubmed]
• Locally misfolded HER2 expressed on cancer cells is a promising target for development of cancer-specific antibodies
• Arimori T, Mihara E, Suzuki H, Ohishi T, Tanaka T, Kaneko MK, Takagi J, Kato Y.
  Structure (2024) 32(5):536-549 [Pubmed]
• C-terminal amino acids in the type I transmembrane domain of L-type lectin VIP36 affect γ-secretase susceptibility
• Hua Z, Watanabe R, Fukunaga T, Matsui Y, Matsuoka M, Yamaguchi S, Tanabe S, Yamamoto M, Kawakami KT, Takagi J, Kajita M, Futai E, Shirakabe K.
  Biochem Biophys Res Commun. (2024) 12:696:149504 [Pubmed]
• Anti-α6β4 integrin autoantibodies inhibit the binding of laminins to α6β4 integrin in patients with pemphigoid and affect the gastrointestinal tract
• Arase N, Sasaoka Y, Narita J, Kiyohara E, Hashimoto K, Shinzaki S, Nojima S, Takagi J, Fujimoto M.
  J Eur Acad Dermatol Venereol. (2024) 38(2):404-412 [Pubmed]
[2023]

• An inhaled ACE2 decoy confers protection against SARS-CoV-2 infection in preclinical models
• Urano E, Itoh Y, Suzuki T, Sasaki T, Kishikawa J, Akamatsu K, Higuchi Y, Sakai Y, Okamura T, Mitoma S, Sugihara F, Takada A, Kimura M, Nakao S, Hirose M, Sasaki T, Koketsu R, Tsuji S, Yanagida S, Shioda T, Hara E, Matoba S, Matsuura Y, Kanda Y, Arase H, Okada M, Takagi J, Kato T, Hoshino A, Yasutomi Y, Saito A, Okamoto T.
  Sci Transl Med. (2023) 15(711):eadi2623 [Pubmed]
• Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex
• Shirai T, Nakai A, Ando E, Fujimoto J, Leach S, Arimori T, Higo D, Eerden F, Tulyeu J, Liu Y, Okuzaki D, Murayama MA, Miyata H, Nunomura K, Lin B, Tani A, Kumanogoh A, Ikawa M, Wing JB, Standley DM, Takagi J, Suzuki K.
  Science Immunology (2023) 8(81):eadc9324 [Pubmed]
• Wnt 2022 EMBO | the Company of Biologists workshop and Yamada conference
• Kikuchi A, Takagi J, Takada S, Ishitani T, Minami Y.
  Genes to Cells (2023) 28(4):249-257 [Pubmed]
• Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein
• Kuhn CC, Basnet N, Bodakuntla S, Alvarez-Brecht P, Nichols S, Martinez-Sanchez A, Agostini L, Soh YM, Takagi J, Biertümpfel C, Mizuno N.
  Nat Commun. (2023) 14(1):620 [Pubmed]
• Advances in tooth agenesis and tooth regeneration
• Ravi V, Murashima-Suginami A, Kiso H, Tokita Y, Huang CL, Bessho K, Takagi J, Sugai M, Tabata Y, Takahashi K.
  Regen Ther. (2023) 3:22:160-168 [Pubmed]
[2022]

• Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions
• Sakai K, Sugano-Nakamura N, Mihara E, Rojas-Chaverra NM, Watanabe S, Sato H, Imamura R, Voon DC, Sakai I, Yamasaki C, Tateno C, Shibata M, Suga H, Takagi J, Matsumoto K.
  Nat Biomed Eng. (2022) 7(2):164-176 [Pubmed]
• Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy
• Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M, Kiuchi T, Takagi J, Watanabe N.
  Cell Reports Methods (2022) 2(10):100301 [Pubmed]
• Structure-Based Discovery of a Novel Class of Small-Molecule Pure Antagonists of Integrin αVβ3
• Sen S, Spasic A, Sinha A, Wang J, Bush M, Li J, Nešić D, Zhou Y, Angiulli G, Morgan P, Salas-Estrada L, Takagi J, Walz T, Coller BS, Filizola M.
  J Chem Inf Model. (2022) 62(22):5607-5621 [Pubmed]
• Hybrid in vitro/in silico analysis of low-affinity protein-protein interactions that regulate signal transduction by Sema6D
• Tanaka T, Ekimoto T, Nagatomo M, Neyazaki M, Shimoji E, Yamane T, Kanagawa S, Oi R, Mihara E, Takagi J, Akashi S, Ikeguchi M, Nogi T.
  Protein Sci. (2022) 31(11):e4452 [Pubmed]
• De novo Fc-based receptor dimerizers differentially modulate PlexinB1 function
• Sugano-Nakamura N, Matoba K, Hirose M, Bashiruddin NK, Matsunaga Y, Yamashita K, Hirata K, Yamamoto M, Arimori T, Suga H, Takagi J.
  Structure (2022) 30(10):1411-1423.e4 [Pubmed]
• Engineering ACE2 decoy receptors to combat viral escapability
• Arimori T, Ikemura N, Okamoto T, Takagi J, Standley DM, Hoshino A.
  Trends Pharmacol Sci. (2022) 43(10):838-851. [Pubmed]
• Functional role of the Frizzled linker domain in the Wnt signaling pathway
• Ko SB, Mihara E, Park Y, Roh K, Kang C, Takagi J, Bang I, Choi HJ.
  Commun Biol. (2022) 5(1):421. [Pubmed]
• An engineered ACE2 decoy neutralizes the SARS-CoV-2 Omicron variant and confers protection against infection in vivo
• Ikemura N, Taminishi S, Inaba T, Arimori T, Motooka D, Katoh K, Kirita Y, Higuchi Y, Li S, Suzuki T, Itoh Y, Ozaki Y, Nakamura S, Matoba S, Standley DM, Okamoto T, Takagi J, Hoshino A.
  Sci Transl Med. (2022) :eabn7737. [Pubmed]
• Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain
• Hasegawa K, Ikeda S, Yaga M, Watanabe K, Urakawa R, Iehara A, Iwai M, Hashiguchi S, Morimoto S, Fujiki F, Nakajima H, Nakata J, Nishida S, Tsuboi A, Oka Y, Yoshihara S, Manabe M, Ichihara H, Mugitani A, Aoyama Y, Nakao T, Hirose A, Hino M, Ueda S, Takenaka K, Masuko T, Akashi K, Maruno T, Uchiyama S, Takamatsu S, Wada N, Morii E, Nagamori S, Motooka D, Kanai Y, Oji Y, Nakagawa T, Kijima N, Kishima H, Ikeda A, Ogino T, Shintani Y, Kubo T, Mihara E, Yusa K, Sugiyama H, Takagi J, Miyoshi E, Kumanogoh A, Hosen N.
  Sci Transl Med. (2022) 14(632):eaax7706. [Pubmed]
[2021]

• Serum autoantibodies against the extracellular region of α6β4 integrin in a patient with dipeptidyl peptidase-4 inhibitor-induced bullous pemphigoid
• Yamashita C, Arase N, Higuchi S, Arase H, Takagi J, Nojima S, Tanemura A, Fujimoto M.
  JAAD Case Rep. (2021) 20:65-68. [Pubmed]
• De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist
• Komatsu Y, Terasaka N, Sakai K, Mihara E, Wakabayashi R, Matsumoto K, Hilvert D, Takagi J, Suga H.
  iScience (2021) 24(11):103302. [Pubmed]
• Structural mechanism of laminin recognition by integrin
• Arimori T, Miyazaki N, Mihara E, Takizawa M, Taniguchi Y, Cabañas C, Sekiguchi K, Takagi J.
  Nat. Commun. (2021) 12(1):4012. [Pubmed]
• Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2
• Higuchi Y, Suzuki T, Arimori T, Ikemura N, Mihara E, Kirita Y, Ohgitani E, Mazda O, Motooka D, Nakamura S, Sakai Y, Itoh Y, Sugihara F, Matsuura Y, Matoba S, Okamoto T, Takagi J, Hoshino A.
  Nat. Commun. (2021) 12(1):3802. [Pubmed]
• Structural insights into integrin α5β1 opening by fibronectin ligand
• Schumacher S, Dedden D, Nunez RV, Matoba K, Takagi J, Biertümpfel C, Mizuno N.
  Sci. Adv. (2021) 7(19):eabe9716. [Pubmed]
• Molecular recognition of a single-chain Fv antibody specific for GA-pyridine, an advanced glycation end-product (AGE), elucidated using biophysical techniques and synthetic antigen analogues
• Kobashigawa Y, Ohara T, Morita K, Toyota Y, Nakamura T, Kotani S, Arimori T, Yamauchi S, Liu C, Kitazaki M, Wakeyama-Miyazaki Y, Suwa Y, Uchida-Kamekura M, Fukuda N, Sato T, Nakajima M, Takagi J, Yamagata Y, Morioka H.
  J. Biochem. (2021) 170(3):379-387. [Pubmed]
• Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins
• Mihara E, Watanabe S, Bashiruddin NK, Nakamura N, Matoba K, Sano Y, Maini R, Yin Y, Sakai K, Arimori T, Matsumoto K, Suga H, Takagi J.
  Nat. Commun. (2021) 12(1):1543. [Pubmed]
• Anti-USAG-1 therapy for tooth regeneration through enhanced BMP signaling
• Murashima-Suginami A, Kiso H, Tokita Y, Mihara E, Nambu Y, Uozumi R, Tabata Y, Bessho K, Takagi J, Sugai M, Takahashi K.
  Sci. Adv. (2021) 7(7):eabf1798. [Pubmed]
• A feasibility study of inverse contrast-matching small-angle neutron scattering method combined with size exclusion chromatography using antibody interactions as model systems
• Sato N, Yogo R, Yanaka S, Martel A, Porcar L, Morishima K, Inoue R, Tominaga T, Arimori T, Takagi J, Sugiyama M, Kato K.
  J. Biochem. (2021) 169(6):701-708. [Pubmed]
[2020]

• Structural studies of reelin N-terminal region provides insights into a unique structural arrangement and functional multimerization
• Nagae M, Suzuki K, Yasui N, Nogi T, Kohno T, Hattori M, Takagi J.
  J. Biochem. (2020) 169(5):555-564. [Pubmed]
• Development of cyclic peptides with potent in vivo osteogenic activity through RaPID-based affinity maturation
• Bashiruddin NK, Hayashi M, Nagano M, Wu Y, Matsunaga Y, Takagi J, Nakashima T, Suga H.
  Proc. Natl. Acad. Sci. U S A. (2020) 117(49):31070-31077. [Pubmed]
• Dissection of α4β7 integrin regulation by Rap1 using novel conformation-specific monoclonal anti-β7 antibodies
• Sato T, Ishihara S, Marui R, Takagi J, Katagiri K.
  Sci. Rep. (2020) 10(1):13221. [Pubmed]
• Negatively charged amino acids in the stalk region of membrane proteins reduce ectodomain shedding
• Iwagishi R, Tanaka R, Seto M, Takagi T, Norioka N, Ueyama T, Kawamura T, Takagi J, Ogawa Y, and Shirakabe K.
  J. Biol. Chem. (2020) 295(35):12343-12352. [Pubmed]
• Site-specific epitope insertion into recombinant proteins using the MAP tag system
• Wakasa A, Kaneko MK, Kato Y, Takagi J, and Arimori T.
  J. Biochem. (2020) 168(4):375-384. [Pubmed]
• Macrocyclic Peptides that Inhibit Wnt Signalling via interaction with Wnt3a
• Otero-Ramirez ME, Matoba K, Mihara E, Passioura T, Takagi J, and Suga H.
  RSC Chemical Biol. (2020) 1, 26-34.
• The constitutive high affinity Met binding site in the kringle domain is dispensable for the signaling activity of hepatocyte growth factor
• Umitsu M, Sakai K, Tamura-Kawakami K, Matsumoto K, Takagi J.
  J Biochem. (2020) 167(6):577-586. [Pubmed]
[2019]

• Novel Pure αVβ3 Antagonists That Do Not Induce Receptor Extension, Prime the Receptor, or Enhance Angiogenesis at Low Concentrations
• Li J, Fukase Y, Shang Y, Zou W, Munoz-Felix JM, Buitrago L, van Agthoven J, Zhang Y, Hara R, Tanaka Y, Okamoto R, Yasui T, Nakahata T, Imaeda T, Aso K, Zhou Y, Locuson C, Nesic D, Duggan M, Takagi J, Vaughan RD, Walz T, Hodivala-Dilke K, Teitelbaum SL, Arnaout MA, Filizola M, Foley MA, Coller BS.
  ACS Pharmacol. Transl. Sci (2019) 2, 387-401. [Pubmed]
• Impaired ligand-dependent MET activation caused by an extracellular SEMA domain missense mutation in lung cancer
• Miao W, Sakai K, Sato H, Imamura R, Jangphattananont N, Takagi J, Nishita M, Minami Y, Matsumoto K.
  Cancer Sci. (2019) 110(10): 3340-3349. [Pubmed]
• Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach
• Jangphattananont N, Sato H, Imamura R, Sakai K, Terakado Y, Murakami K, Barker N, Oshima H, Oshima M, Takagi J, Kato Y, Yano S, Matsumoto K.
  Int J Mol Sci. (2019) 20(12). pii: E2955. [Pubmed]
• Crystal structure of mammalian Wnt-frizzled complex
• Hirai H, Matoba K, Mihara E, Arimori T, and Junichi Takagi J
  Nature Struct. Mol. Biol. (2019) 26(5):372-379. [Pubmed]
• Structural basis of sarco/endoplasmic reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay
• Inoue M, Sakuta N, Watanabe S, Yoshikaie K, Tanaka Y, Ushioda R, Kato Y, Takagi J, Tsukazaki T, Nagata K, and Inaba K
  Cell Rep. (2019) 27(4):1221-1230.e3. [Pubmed]
• Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor
• Sakai K, Passioura T, Sato H, Ito K, Furuhashi H, Umitsu M, Takagi J, Kato Y, Yano S, Shibata M, Suga H, and Matsumoto K
  Nature Chem. Biol. (2019) 15 (6):598-606. [Pubmed]
• Structural basis for oligomerization of the prokaryotic peptide transporter PepTSo2
• Nagamura R, Fukuda M, Kawamoto A, Matoba K, Naoshi Dohmae N, Ishitani R, Takagi J, and Nureki O
  Acta Crystallogr. F (2019) 75(Pt 5):348-358. [Pubmed]
• Advances in domain and subunit localization technology for electron microscopy
• Brown ZP, Takagi J.
  Biophys Rev. (2019) 11(2):149-155. [Pubmed]
[2018]

• The PA Ttag: A versatile peptide tagging system in the era of integrative structural biology
• Brown ZP, Takagi J.
  Adv Exp Med Biol. (2018) 1105:59-76. [Pubmed]
• Cellular signaling and gene expression profiles evoked by a bivalent macrocyclic peptide that serves as an artificial MET receptor agonist
• Miao W, Sakai K, Ozawa N, Nishiuchi T, Suzuki Y, Ito K, Morioka T, Umitsu M, Takagi J, Suga H, and Matsumoto K.
  Sci Rep. (2018) 8(1):16492. [Pubmed]
• Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy
• Tsunoda J, Song C, Imai FL, Takagi J, Ueno H, Murata T, Iino R, and Murata K.
  Sci Rep. (2018) 8(1):15632. [Pubmed]
• Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V
• Nagae M, Kizuka Y, Mihara E, Kitago Y, Hanashima S, Ito Y, Takagi J, Taniguchi N, and Yamaguchi Y.
  Nat Commun. (2018) 9(1): 3380. [Pubmed]
• Semaphorin 6D reverse signaling controls macrophage lipid metabolism and anti-inflammatory polarization
• Kang S, Nakanishi Y, Kioi Y, Okuzaki D, Kimura T, Takamatsu H, Koyama S, Nojima S, Nishide M, Hayama Y, Kinehara Y, Kato Y, Nakatani T, Shimogori T, Takagi J, Toyofuku T, and Kumanogoh A.
  Nature Immunology (2018) 19 (6): 561-570. [Pubmed]
• Facile synthesis of dimeric thioether-macrocyclic peptides with antibody-like affinity against Plexin-B1
• Bashiruddin N, Matsunaga Y, Nagano M, Takagi J, and Suga H.
  Bioconjug Chem. (2018) 29(6): 1847-1851. [Pubmed]
• Systematic survey of conformational states in 1 and 4 integrins by negative-stain electron microscopy
• Miyazaki N, Iwasaki K, and Takagi J.
  J. Cell Sci. (2018) 131(10): jcs.216754. [Pubmed]
• Yeast inner-subunit PA-NZ-1 labeling strategy for accurate subunit identification in a macromolecular complex through cryo-EM analysis
• Wang H, Han W, Takagi J, and Cong Y.
  J. Mol. Biol. (2018) 430 (10), 1417-1425. [Pubmed]
• Foreword to 'Multiscale structural biology: biophysical principles and mechanisms underlying the action of bio-nanomachines', a special issue in Honour of Fumio Arisaka's 70th birthday
• Hall D, Takagi J, and Nakamura H.
  Biophys. Rev. (2018) 10(2):105-129. [Pubmed]
• An anti-peptide monoclonal antibody recognizing the tobacco etch virus protease-cleavage sequence and its application to a tandem tagging system
• Tabata S, Kitago Y, Fujii Y, Mihara E, Tamura-Kawakami K, Norioka N, Takahashi K, Kaneko MK, Kato Y, and Takagi J.
  Protein Expr Purif. (2018) 147, 94-99. [Pubmed]
• Human pancreatic tumor organoids reveal loss of stem cell niche factor dependence during disease progression
• Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, Ohta Y, Matano M, Nanki K, Kawasaki K, Takahashi S, Sugimoto S, Iwasaki E, Takagi J, Itoi T, Kitago M, Kitagawa Y, Kanai T, and Sato T.
  Cell Stem Cell (2018) 22(3), 454-467. [Pubmed]
• Development of a new protein labeling system to map subunits and domains of macromolecular complexes for electron microscopy
• Brown ZP, Arimori T, Iwasaki K, and Takagi J.
  J. Struct. Biol. (2018) 201, 247-251. [Pubmed]
[2017]

• Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus
• Sakakibara S, Arimori T, Yamashita K, Jinzai H, Motooka D, Nakamura S, Li S, Takeda K, Katayama J, Hussien MAE, Narazaki M, Tanaka T, Standley D, Takagi J, and Kikutani H.
  Scientific Rep. (2017) 7: 16428. [Pubmed]
• Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors
• Saito F, Hirayasu K, Satoh T, Wang CW3, Lusingu J, Arimori T, Shida K, Palacpac NMQ, Itagaki S, Iwanaga S, Takashima E, Tsuboi T, Kohyama M, Suenaga T, Colonna M, Takagi J, Lavstsen T, Horii T, and Arase H.
  Nature (2017) 552(7683): 101-105. [Pubmed]
• Molecular dissection of protein-protein interactions between integrin α5β1 and the Helicobacter pylori Cag type IV secretion system
• Koelblen T, Berge C, Cherrier MV, Brillet K, Jimenez-Soto L, Ballut L, Takagi J, Montserret R, Rousselle P, Fischer W, Haas R, Fronzes R, and Terradot L.
  FEBS J. (2017) 284(23): 4143-4157. [Pubmed]
• The activated conformation of integrin β7 as a target for multiple myeloma-specific chimeric antigen receptor T-cell therapy
• Hosen N, Matsunaga Y, Hasegawa K, Matsuno H, Nakamura Y, Makita M, Watanabe K, Yoshida M, Satoh K, Morimoto S, Fujiki F, Nakajima H, Nakata J, Nishida S, Tsuboi A, Oka Y, Manabe M, Ichihara H, Aoyama Y, Mugitani A, Nakao T, Hino M, Uchibori R, Ozawa K, Baba Y, Terakura S, Wada N, Morii E, Nishimura J, Takeda K, Oji Y, Sugiyama H, Takagi J, and Kumanogoh A.
  Nature Medicine (2017) 23: 1436-1443. [Pubmed]
• Fv-clasp: An artificially designed small antibody fragment with improved production compatibility, stability, and crystallizability
• Arimori, T, Kitago, Y, Umitsu, M, Fujii, Y, Asaki, R, Tamura-Kawakami, K, and Takagi, J.
  Structure (2017) 25: 1611-1622. [Pubmed]
• Mechanistic basis for the recognition of laminin-511 by a6b1 integrin
• Takizawa, M, Arimori, T, Taniguchi, Y, Kitago, Y, Yamashita, E, Takagi, J, and Sekiguchi, K.
  Science Advances (2017) 3(9): e1701497. [Pubmed]
• Loss of Parkinson’s disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c
• Meng H, Yamashita C, Shiba-Fukushima K, Inoshita T, Funayama M, Sato S, Hatta T, Natsume T, Umitsu M, Takagi J, Imai Y, Hattori N.
  Nature Commun. (2017) 8: 15500. [Pubmed]
• Structural basis for perception of diverse chemical substances by T1r taste receptors
• Nuemket N, Yasui N, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko M.K, Takagi J, Hosotani M, Yamashita A.
  Nature Commun. (2017) 8: 15530. [Pubmed]
• Structural basis for ligand capture and release by the endocytic receptor ApoER2
• Hirai H, Yasui N, Yamashita K, Tabata S, Yamamoto M, Takagi J, Nogi T.
  EMBO Rep. (2017) pii: e201643521. [Pubmed]
• Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation
• Shirakabe K, Omura T, Shibagaki Y, Mihara E, Homma K, Kato Y, Yoshimura A, Murakami Y, Takagi J, Hattori S, Ogawa Y.
  Scientific Rep. (2017) 7:46174. [Pubmed]
• The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2
• Fukuda S, Kita S, Obata Y, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nishizawa H, Funahashi T, Takagi J, Maeda N, and Shimomura I.
  J Biol Chem. (2017) 292 (19), 7840-7849. [Pubmed]
• 2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching
• Matsumoto A, Miyazaki N, Takagi J, and Iwasaki K.
  Scientific Rep. (2017) 7(1):377. [Pubmed]
• Conformational freedom of the LRP6 ectodomain is regulated by N-glycosylation and the binding of the Wnt antagonist Dkk1
• Matoba K, Mihara E, Tamura-Kawakami K, Miyazaki N, Maeda S, Hirai H, Thompson S, Iwasaki K, and Takagi J.
  Cell Reports (2017) 18, 32-40. [Pubmed]
[2016]

• Allosteric inhibition of a semaphorin 4D receptor plexin B1 by a high-affinity macrocyclic peptide
• Matsunaga Y, Bashiruddin NK, Kitago Y, Takagi J* and Suga H.
  Cell Chem. Biol. (2016) 23:1341-1350. [Pubmed]
• Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies
• Umitsu M, Ogasawara S, Sakai K, Kaneko M, Asaki R, Tamura-Kawakami K, Kato Y, Matsumoto K, and Takagi J.
  Scientific Rep. (2016) 9 (6):33149. [Pubmed]
• Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by ERdj5
• Ushioda R, Miyamoto A, Inoue M, Watanabe S, Okumura M, Maegawa K, Uegaki K, Fujii S, Fukuda Y, Umitsu M, Takagi J, Inaba K, Mikoshiba K, and Nagata K.
  Proc. Natl. Acad. Sci. USA (2016) 113(41):E6055-E6063. [Pubmed]
• Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1
• Kitago Y, Kaneko MK, Ogasawara S, Kato Y, and Takagi J.
  Biochem Biophys Res Commun. (2016) 478(3):1274-9. [Pubmed]
• Molecular Basis of the Ligand Binding Specificity of αvβ8 Integrin
• Ozawa A, Sato Y, Imabayashi T, Uemura T, Takagi J, and Sekiguchi K.
  J Biol Chem. (2016) 291(22):11551-65. [Pubmed]
• Structure of the plexin ectodomain bound by semaphorin-mimicking antibodies
• Suzuki K, Tsunoda H, Omiya R, Matoba K, Baba T, Suzuki S, Segawa H, Kumanogoh A, Iwasaki K, Hattori K, Takagi J.
  PLoS One (2016) 11 (6):e0156719. [Pubmed]
• Tailored placement of a turn-forming PA tag into the structured domain of a protein to probe its conformational state
• Fujii Y, Matsunaga Y, Arimori T, Kitago Y, Ogasawara S, Kaneko M, Kato Y, and Takagi J.
  J. Cell Science (2016) 129:1512-1522. [Pubmed]
• Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin
• Mihara E, Hirai H, Yamamoto H, Tamura-Kawakami K, Matano M, Kikuchi A, Sato T, and Takagi J.
  eLife (2016) 5:e11621. [Pubmed]
• Structural and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase
• Morita J, Kano K, Kato K, Takita H, Skagami H, Yamamoto Y, Mihara E, Ueda H, Sato T, Tokuyama H, Arai H, Asou H, Takagi J, Ishitani R, Nishimasu H, Nureki O, and Aoki J.
  Scientific Rep. (2016) 6:20995. [Pubmed]
[2015]

• Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration
• Kharitidi D, Apaja PM, Manteghi S, Suzuki K, Malitskaya E, Roldan A, Gingras MC, Takagi J, Lukacs GL, AND Pause A.
  Cell Rep. (2015) 13(3):599-609. [Pubmed]
• Structural basis for amyloidogenic peptide recognition by sorLA
• Kitago Y, Nagae M, Nakata Z, Yagi-Utsumi M, Takagi-Niidome S, Mihara E, Nogi T, Kato K, AND Takagi J.
  Nature Struct. Mol. Biol. (2015) 22:199-206. [Pubmed]
[2014]

• Editorial overview: New constructs and expression of proteins: Making things better
• Takagi, J. and Tate, CG.
  Curr Opin Struct Biol. (2014) 26:iv-vi. [Pubmed]
• Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6
• Morita J. et al.
  Acta Crystallogr. F Struct. Biol. Commun. (2014) 70:794-799. [Pubmed]
• PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin
• Fujii, Y. et al.
  Protein Exp. Purif. (2014) 95:240-247. [Pubmed]
• Lysosomal sorting of amyloid-β by the SORLA receptor is impaired by a familial Alzheimer’s disease mutation
• Caglayan, S. et al.
  Science Transl. Med. (2014) 6:223ra20. [Pubmed]
• A structure-based model of substrate discrimination by a non-canonical PDZ tandem in the intramembrane-cleaving protease RseP
• Hizukuri, Y. et al.
  Structure (2014) 22:326-336. [Pubmed]
• Arl6IP1 has the ability to shape the mammalian ER membrane in a reticulon-like fashion
• Yamamoto, Y. et al.
  Biochem. J. (2014) 458:69-79. [Pubmed]
[2013]

• Life cycle of phytoreoviruses visualized by electron microscopy and tomography
• Miyazaki, N. et al.
  Front. Microbiol. (2013) 4:1-9. [Pubmed]
• Cryo-electron tomography: moving towards revealing the viral life cycle of Rice dwarf virus
• Miyazaki, N. et al.
  J. Synchrotron Radiat. (2013) 20:826-828. [Pubmed]
• Crystal structure and enzymatic activity of an ADAMTS13 mutant with the East Asian-specific P475S polymorphism
• Akiyama M. et al.
  J Thromb Haemost. (2013) 11:1399-1406. [Pubmed]
• A multi-purpose fusion tag derived from an unstructured and hyper-acidic region of the amyloid precursor protein
• Sangawa T. et al.
  Protein Sci. (2013) 22:840-850. [Pubmed]
• A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration
• Nojima S. et al.
  Nature Commun. (2013) 4:1406 [Pubmed]
[2012]

• Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling
• Kato K. et al.
  Proc. Natl. Acad. Sci. USA (2012) 109:16876-16881. [Pubmed]
• Schwann Cell Myelination Requires Integration of Laminin Activities
• McKee, K.K. et al.
  J. Cell Sci. (2012) 125:4609-4619. [Pubmed]
• Higher-order architecture of cell adhesion mediated by polymorphic synaptic adhesion molecules neurexin and neuroligin
• Tanaka, H. et al.
  Cell Reports (2012) 2:101-110. [Pubmed]
• Gold Nanoparticle-Induced Formation of Artificial Protein Capsids
• Malay, A.D. et al.
  Nano Lett (2012) 12:2056-2059. [Pubmed]
• Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1
• Kato K. et al.
  Acta Crystallogr. F (2012) 68:778-782. [Pubmed]
• Crystal structure of α5β1 integrin ectodomain: Atomic details of the fibronectin receptor
• Nagae, M. et al.
  J. Cell Biol. (2012) 197:131-140. [Pubmed]
• Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer
• Matoba K. et al.
  J Electron Microsc (Tokyo) (2012) 61:193-198. [Pubmed]
• Neutralization of the g-secretase activity by monoclonal antibody against extracellular domain of nicastrin
• Hayashi, I. et al.
  Oncogene (2012) 31:787-798. [Pubmed]
[2011]

• Functional importance of covalent homodimer of reelin protein linked via its central region
• Yasui, N. et al.
  J. Biol. Chem. (2011) 286:35247-35256. [Pubmed]
• Structural Basis for Variant-Specific Neuroligin-Binding by α-Neurexin
• Tanaka, H. et al.
  PLoS One (2011) 6:e19411[Pubmed]
• Crystal structure of the cis-dimer of nectin-1: implications for the architecture of cell-cell junctions
• Narita, H. et al.
  J.Biol.Chem. (2011) 286:12659-12669. [Pubmed]
• Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure
• Akita, F. et al.
  J. Gen. Virol. (2011) 92:2214-2221. [Pubmed]
• Three-dimensional analysis of the association of viral particles with mitochondria during the replication of Rice gall dwarf virus
• Wei, T. et al.
  J. Mol. Biol. (2011) 410(3):436-446. [Pubmed]
• Crystal structure of Autotaxin, a secreted lysophospholipase D that generates GPCR-signaling lipid mediator
• Nishimasu, H. et al.
  Nature Struct. Mol. Biol. (2011) 18:205-212. [Pubmed]
• Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain
• Nakata, Z. et al.
  Acta Crystallographica. (2011) F67:129-132. [Pubmed]
[2010]

• Electron tomography of the supramolecular structure of virus-infected cells
• Iwasaki, K. and Omura, T.
  Curr.Opin.Struct.Biol. (2010) 50:632-639. [Pubmed]
• Tomosyn Inhibits Synaptotagmin-1-mediated Step of Ca2+-dependent Neurotransmitter Release through Its N-terminal WD40 Repeats
• Yamamoto, Y. et al.
  J. Biol. Chem. (2010) 285:40943-40955. [Pubmed]
• Structural basis for semaphorin signaling through the plexin receptor
• Nogi, T. et al.
  Nature (2010) 467:1123-1127. [PubMed]
• Comparison of the envelope architecture of E. coli using two methods: CEMOVIS and cryo-electron tomography
• Kishimoto-Okada, A. et al.
  J. Electron Microsc (2010) 59:419-426. [PubMed]
• Laminin-121-Recombinant expression and interactions with integrins
• Sasaki, T. et al.
  Matrix Biology (2010) 29:484-493. [PubMed]
• A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system
• Tabata, S. et al.
  J. Proteomics. (2010) 73:1777-1785. [PubMed]
• Structural basis for specific recognition of reelin by its receptors
• Yasui, N. et al.
  Structure (2010) 18:320-331.[PubMed]
• Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation
• Yasui, N. et al.
  J. Immunol. Methods (2010) 352:153-160. [PubMed]
[2009]

• The structure of rat liver vault at 3.5 angstrom resolution
• Tanaka, H. et al.
  Science (2009) 323:384-388. [PubMed]
• Molecular dynamism of Fe-S cluster biosynthesis implicated by the structure of SufC2-SufD2 complex
• Wada, K. et al.
  J. Mol. Biol. (2009) 387:245-258. [PubMed]
• Molecular basis of the recognition of nephronectin by integrin alpha8beta1
• Sato, Y. et al.
  J. Biol. Chem. (2009) 284:14524-14536. [PubMed]
• Production, crystallization and preliminary crystallographic analysis of the exosite-containing fragment of human von Willebrand factor-cleaving proteinase, ADAMTS13
• Akiyama, M. et al.
  Acta Crystallographica (2009) F65:739-742. [PubMed]
• Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor
• Akiyama, M. et al.
  Proc. Natl. Acad. Sci. USA (2009) 106:19274-19279.[PubMed]
• A self-assembled protein nanotube with high aspect ratio
• Miranda, F. et al.
  Small (2009) 5:2077-2084. [PubMed]
• Association of Rice gall dwarf virus with microtubules is necessary for viral release from cultured insect vector cells
• Wei, T., et al.
  J. Virol. (2009) 83:10830-10835. [PubMed]
[2008]

• Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus
• Yamaguchi, H. et al.
  J. Leukocyte Biol. (2008) 83:1300-1307. [PubMed]
• Key interactions in ectodomain of integrin for global conformational change detected by elastic network normal mode analysis
• Matsumoto, A. et al.
  Biophys. J. (2008) 95(6):2895-2908. [PubMed]
• Structure of F-spondin reeler domain reveals a unique b-sandwich fold with a deformable disulfide-bonded loop
• Nagae, M. et al.
  Acta Crystallographica. (2008) D64:1138-1145. [PubMed]
• A murine monoclonal antibody that binds N-terminal extracellular segment of human protease activated receptor-4
• Sangawa, T. et al.
  Hybridoma (2008) 27(5):331-335. [PubMed]
• Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification
• Nogi, T. et al.
  Protein Sci. (2008) 17:2120-2126. [PubMed]
• A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
• Kato, K. et al.
  Acta Crystallogr. (2008) D64:525-531. [PubMed]
• Pleomorphic Configuration of the Trimeric Capsid Proteins of Rice dwarf virus that Allows Formation of Both the Outer Capsid and Tubular Crystals
• Iwasaki, K. et al.
  J.Mol.Biol. (2008) 383:252-265. [PubMed]
[2007]

• Structural basis for ligand recognition by integrins
• Takagi, J.
  Curr. Opin. Cell Biol. (2007) 19:557-564.[PubMed]
• Structure of a receptor-binding reelin fragment and mutational analysis reveal a recognition mechanism similar to endocytic receptors
• Yasui, N. et al.
  Proc. Natl. Acad. Sci. USA (2007) 104:9988-9993.[PubMed]
• The RGD motif in fibronectin is essential for mouse development but dispensable for fibronectin fibril assembly
• Takahashi, S. et al.
  J. Cell Biol. (2007) 178:167-178. [PubMed]
• Three-dimensional architecture of virus-packed tubule
• Katayama, S. et al.
  J. Electron Microsc. (2007) 56:77-81. [PubMed]
• Semaphorin 7A initiates T cell-mediated inflammatory responses through alpha1beta1 integrin
• Suzuki K. et al.
  Nature (2007) 446:680-684. [PubMed]
[2006]

• alphaVbeta6 is a novel receptor for human fibrillin-1: comparative studies of molecular determinants underlying integrin-RGD affinity and specificity
• Jovanovic, J. et al.
  J. Biol. Chem. (2006) 282:6743-6751. [PubMed]
• Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography
• Nogi, T. et al.
  EMBO J. (2006) 25:3675-3683. [PubMed]
• Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation
• Tarui, T. et al.
  Thromb Haemost. (2006) 95:524-534. [PubMed]
• Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins
• Nishiuchi, R. et al.
  Matrix Biol. (2006) 25:189-197.[PubMed]
[2005]

• Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering
• Luo, B. H. et al.
  Proc. Natl. Acad. Sci. USA (2005) 102:3679-3684. [PubMed]
• Potentiation of the ligand-binding activity of integrin alpha3 beta1 via association with tetraspanin CD151
• Nishiuchi, R. et al.
  Proc. Natl. Acad. Sci. USA (2005) 102:1939-1944. [PubMed]

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