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【論文】

    [2022]
  • An engineered ACE2 decoy neutralizes the SARS-CoV-2 Omicron variant and confers protection against infection in vivo
  • Ikemura N, Taminishi S, Inaba T, Arimori T, Motooka D, Katoh K, Kirita Y, Higuchi Y, Li S, Suzuki T, Itoh Y, Ozaki Y, Nakamura S, Matoba S, Standley DM, Okamoto T, Takagi J, Hoshino A.
    Sci Transl Med. (2022) :eabn7737. [Pubmed]
  • Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain
  • Hasegawa K, Ikeda S, Yaga M, Watanabe K, Urakawa R, Iehara A, Iwai M, Hashiguchi S, Morimoto S, Fujiki F, Nakajima H, Nakata J, Nishida S, Tsuboi A, Oka Y, Yoshihara S, Manabe M, Ichihara H, Mugitani A, Aoyama Y, Nakao T, Hirose A, Hino M, Ueda S, Takenaka K, Masuko T, Akashi K, Maruno T, Uchiyama S, Takamatsu S, Wada N, Morii E, Nagamori S, Motooka D, Kanai Y, Oji Y, Nakagawa T, Kijima N, Kishima H, Ikeda A, Ogino T, Shintani Y, Kubo T, Mihara E, Yusa K, Sugiyama H, Takagi J, Miyoshi E, Kumanogoh A, Hosen N.
    Sci Transl Med. (2022) 14(632):eaax7706. [Pubmed]
  • [2021]
  • Serum autoantibodies against the extracellular region of α6β4 integrin in a patient with dipeptidyl peptidase-4 inhibitor-induced bullous pemphigoid
  • Yamashita C, Arase N, Higuchi S, Arase H, Takagi J, Nojima S, Tanemura A, Fujimoto M.
    JAAD Case Rep. (2021) 20:65-68. [Pubmed]
  • De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist
  • Komatsu Y, Terasaka N, Sakai K, Mihara E, Wakabayashi R, Matsumoto K, Hilvert D, Takagi J, Suga H.
    iScience (2021) 24(11):103302. [Pubmed]
  • Structural mechanism of laminin recognition by integrin
  • Arimori T, Miyazaki N, Mihara E, Takizawa M, Taniguchi Y, Cabañas C, Sekiguchi K, Takagi J.
    Nat. Commun. (2021) 12(1):4012. [Pubmed]
  • Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2
  • Higuchi Y, Suzuki T, Arimori T, Ikemura N, Mihara E, Kirita Y, Ohgitani E, Mazda O, Motooka D, Nakamura S, Sakai Y, Itoh Y, Sugihara F, Matsuura Y, Matoba S, Okamoto T, Takagi J, Hoshino A.
    Nat. Commun. (2021) 12(1):3802. [Pubmed]
  • Structural insights into integrin α5β1 opening by fibronectin ligand
  • Schumacher S, Dedden D, Nunez RV, Matoba K, Takagi J, Biertümpfel C, Mizuno N.
    Sci. Adv. (2021) 7(19):eabe9716. [Pubmed]
  • Molecular recognition of a single-chain Fv antibody specific for GA-pyridine, an advanced glycation end-product (AGE), elucidated using biophysical techniques and synthetic antigen analogues
  • Kobashigawa Y, Ohara T, Morita K, Toyota Y, Nakamura T, Kotani S, Arimori T, Yamauchi S, Liu C, Kitazaki M, Wakeyama-Miyazaki Y, Suwa Y, Uchida-Kamekura M, Fukuda N, Sato T, Nakajima M, Takagi J, Yamagata Y, Morioka H.
    J. Biochem. (2021) 170(3):379-387. [Pubmed]
  • Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins
  • Mihara E, Watanabe S, Bashiruddin NK, Nakamura N, Matoba K, Sano Y, Maini R, Yin Y, Sakai K, Arimori T, Matsumoto K, Suga H, Takagi J.
    Nat. Commun. (2021) 12(1):1543. [Pubmed]
  • Anti-USAG-1 therapy for tooth regeneration through enhanced BMP signaling
  • Murashima-Suginami A, Kiso H, Tokita Y, Mihara E, Nambu Y, Uozumi R, Tabata Y, Bessho K, Takagi J, Sugai M, Takahashi K.
    Sci. Adv. (2021) 7(7):eabf1798. [Pubmed]
  • A feasibility study of inverse contrast-matching small-angle neutron scattering method combined with size exclusion chromatography using antibody interactions as model systems
  • Sato N, Yogo R, Yanaka S, Martel A, Porcar L, Morishima K, Inoue R, Tominaga T, Arimori T, Takagi J, Sugiyama M, Kato K.
    J. Biochem. (2021) 169(6):701-708. [Pubmed]
  • [2020]
  • Structural studies of reelin N-terminal region provides insights into a unique structural arrangement and functional multimerization
  • Nagae M, Suzuki K, Yasui N, Nogi T, Kohno T, Hattori M, Takagi J.
    J. Biochem. (2020) 169(5):555-564. [Pubmed]
  • Development of cyclic peptides with potent in vivo osteogenic activity through RaPID-based affinity maturation
  • Bashiruddin NK, Hayashi M, Nagano M, Wu Y, Matsunaga Y, Takagi J, Nakashima T, Suga H.
    Proc. Natl. Acad. Sci. U S A. (2020) 117(49):31070-31077. [Pubmed]
  • Dissection of α4β7 integrin regulation by Rap1 using novel conformation-specific monoclonal anti-β7 antibodies
  • Sato T, Ishihara S, Marui R, Takagi J, Katagiri K.
    Sci. Rep. (2020) 10(1):13221. [Pubmed]
  • Negatively charged amino acids in the stalk region of membrane proteins reduce ectodomain shedding
  • Iwagishi R, Tanaka R, Seto M, Takagi T, Norioka N, Ueyama T, Kawamura T, Takagi J, Ogawa Y, and Shirakabe K.
    J. Biol. Chem. (2020) 295(35):12343-12352. [Pubmed]
  • Site-specific epitope insertion into recombinant proteins using the MAP tag system
  • Wakasa A, Kaneko MK, Kato Y, Takagi J, and Arimori T.
    J. Biochem. (2020) 168(4):375-384. [Pubmed]
  • Macrocyclic Peptides that Inhibit Wnt Signalling via interaction with Wnt3a
  • Otero-Ramirez ME, Matoba K, Mihara E, Passioura T, Takagi J, and Suga H.
    RSC Chemical Biol. (2020) 1, 26-34.
  • The constitutive high affinity Met binding site in the kringle domain is dispensable for the signaling activity of hepatocyte growth factor
  • Umitsu M, Sakai K, Tamura-Kawakami K, Matsumoto K, Takagi J.
    J Biochem. (2020) 167(6):577-586. [Pubmed]
  • [2019]
  • Novel Pure αVβ3 Antagonists That Do Not Induce Receptor Extension, Prime the Receptor, or Enhance Angiogenesis at Low Concentrations
  • Li J, Fukase Y, Shang Y, Zou W, Munoz-Felix JM, Buitrago L, van Agthoven J, Zhang Y, Hara R, Tanaka Y, Okamoto R, Yasui T, Nakahata T, Imaeda T, Aso K, Zhou Y, Locuson C, Nesic D, Duggan M, Takagi J, Vaughan RD, Walz T, Hodivala-Dilke K, Teitelbaum SL, Arnaout MA, Filizola M, Foley MA, Coller BS.
    ACS Pharmacol. Transl. Sci (2019) 2, 387-401. [Pubmed]
  • Impaired ligand-dependent MET activation caused by an extracellular SEMA domain missense mutation in lung cancer
  • Miao W, Sakai K, Sato H, Imamura R, Jangphattananont N, Takagi J, Nishita M, Minami Y, Matsumoto K.
    Cancer Sci. (2019) 110(10): 3340-3349. [Pubmed]
  • Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach
  • Jangphattananont N, Sato H, Imamura R, Sakai K, Terakado Y, Murakami K, Barker N, Oshima H, Oshima M, Takagi J, Kato Y, Yano S, Matsumoto K.
    Int J Mol Sci. (2019) 20(12). pii: E2955. [Pubmed]
  • Crystal structure of mammalian Wnt-frizzled complex
  • Hirai H, Matoba K, Mihara E, Arimori T, and Junichi Takagi J
    Nature Struct. Mol. Biol. (2019) 26(5):372-379. [Pubmed]
  • Structural basis of sarco/endoplasmic reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay
  • Inoue M, Sakuta N, Watanabe S, Yoshikaie K, Tanaka Y, Ushioda R, Kato Y, Takagi J, Tsukazaki T, Nagata K, and Inaba K
    Cell Rep. (2019) 27(4):1221-1230.e3. [Pubmed]
  • Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor
  • Sakai K, Passioura T, Sato H, Ito K, Furuhashi H, Umitsu M, Takagi J, Kato Y, Yano S, Shibata M, Suga H, and Matsumoto K
    Nature Chem. Biol. (2019) 15 (6):598-606. [Pubmed]
  • Structural basis for oligomerization of the prokaryotic peptide transporter PepTSo2
  • Nagamura R, Fukuda M, Kawamoto A, Matoba K, Naoshi Dohmae N, Ishitani R, Takagi J, and Nureki O
    Acta Crystallogr. F (2019) 75(Pt 5):348-358. [Pubmed]
  • Advances in domain and subunit localization technology for electron microscopy
  • Brown ZP, Takagi J.
    Biophys Rev. (2019) 11(2):149-155. [Pubmed]
  • [2018]
  • The PA Ttag: A versatile peptide tagging system in the era of integrative structural biology
  • Brown ZP, Takagi J.
    Adv Exp Med Biol. (2018) 1105:59-76. [Pubmed]
  • Cellular signaling and gene expression profiles evoked by a bivalent macrocyclic peptide that serves as an artificial MET receptor agonist
  • Miao W, Sakai K, Ozawa N, Nishiuchi T, Suzuki Y, Ito K, Morioka T, Umitsu M, Takagi J, Suga H, and Matsumoto K.
    Sci Rep. (2018) 8(1):16492. [Pubmed]
  • Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy
  • Tsunoda J, Song C, Imai FL, Takagi J, Ueno H, Murata T, Iino R, and Murata K.
    Sci Rep. (2018) 8(1):15632. [Pubmed]
  • Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V
  • Nagae M, Kizuka Y, Mihara E, Kitago Y, Hanashima S, Ito Y, Takagi J, Taniguchi N, and Yamaguchi Y.
    Nat Commun. (2018) 9(1): 3380. [Pubmed]
  • Semaphorin 6D reverse signaling controls macrophage lipid metabolism and anti-inflammatory polarization
  • Kang S, Nakanishi Y, Kioi Y, Okuzaki D, Kimura T, Takamatsu H, Koyama S, Nojima S, Nishide M, Hayama Y, Kinehara Y, Kato Y, Nakatani T, Shimogori T, Takagi J, Toyofuku T, and Kumanogoh A.
    Nature Immunology (2018) 19 (6): 561-570. [Pubmed]
  • Facile synthesis of dimeric thioether-macrocyclic peptides with antibody-like affinity against Plexin-B1
  • Bashiruddin N, Matsunaga Y, Nagano M, Takagi J, and Suga H.
    Bioconjug Chem. (2018) 29(6): 1847-1851. [Pubmed]
  • Systematic survey of conformational states in 1 and 4 integrins by negative-stain electron microscopy
  • Miyazaki N, Iwasaki K, and Takagi J.
    J. Cell Sci. (2018) 131(10): jcs.216754. [Pubmed]
  • Yeast inner-subunit PA-NZ-1 labeling strategy for accurate subunit identification in a macromolecular complex through cryo-EM analysis
  • Wang H, Han W, Takagi J, and Cong Y.
    J. Mol. Biol. (2018) 430 (10), 1417-1425. [Pubmed]
  • Foreword to 'Multiscale structural biology: biophysical principles and mechanisms underlying the action of bio-nanomachines', a special issue in Honour of Fumio Arisaka's 70th birthday
  • Hall D, Takagi J, and Nakamura H.
    Biophys. Rev. (2018) 10(2):105-129. [Pubmed]
  • An anti-peptide monoclonal antibody recognizing the tobacco etch virus protease-cleavage sequence and its application to a tandem tagging system
  • Tabata S, Kitago Y, Fujii Y, Mihara E, Tamura-Kawakami K, Norioka N, Takahashi K, Kaneko MK, Kato Y, and Takagi J.
    Protein Expr Purif. (2018) 147, 94-99. [Pubmed]
  • Human pancreatic tumor organoids reveal loss of stem cell niche factor dependence during disease progression
  • Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, Ohta Y, Matano M, Nanki K, Kawasaki K, Takahashi S, Sugimoto S, Iwasaki E, Takagi J, Itoi T, Kitago M, Kitagawa Y, Kanai T, and Sato T.
    Cell Stem Cell (2018) 22(3), 454-467. [Pubmed]
  • Development of a new protein labeling system to map subunits and domains of macromolecular complexes for electron microscopy
  • Brown ZP, Arimori T, Iwasaki K, and Takagi J.
    J. Struct. Biol. (2018) 201, 247-251. [Pubmed]
  • [2017]
  • Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus
  • Sakakibara S, Arimori T, Yamashita K, Jinzai H, Motooka D, Nakamura S, Li S, Takeda K, Katayama J, Hussien MAE, Narazaki M, Tanaka T, Standley D, Takagi J, and Kikutani H.
    Scientific Rep. (2017) 7: 16428. [Pubmed]
  • Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors
  • Saito F, Hirayasu K, Satoh T, Wang CW3, Lusingu J, Arimori T, Shida K, Palacpac NMQ, Itagaki S, Iwanaga S, Takashima E, Tsuboi T, Kohyama M, Suenaga T, Colonna M, Takagi J, Lavstsen T, Horii T, and Arase H.
    Nature (2017) 552(7683): 101-105. [Pubmed]
  • Molecular dissection of protein-protein interactions between integrin α5β1 and the Helicobacter pylori Cag type IV secretion system
  • Koelblen T, Berge C, Cherrier MV, Brillet K, Jimenez-Soto L, Ballut L, Takagi J, Montserret R, Rousselle P, Fischer W, Haas R, Fronzes R, and Terradot L.
    FEBS J. (2017) 284(23): 4143-4157. [Pubmed]
  • The activated conformation of integrin β7 as a target for multiple myeloma-specific chimeric antigen receptor T-cell therapy
  • Hosen N, Matsunaga Y, Hasegawa K, Matsuno H, Nakamura Y, Makita M, Watanabe K, Yoshida M, Satoh K, Morimoto S, Fujiki F, Nakajima H, Nakata J, Nishida S, Tsuboi A, Oka Y, Manabe M, Ichihara H, Aoyama Y, Mugitani A, Nakao T, Hino M, Uchibori R, Ozawa K, Baba Y, Terakura S, Wada N, Morii E, Nishimura J, Takeda K, Oji Y, Sugiyama H, Takagi J, and Kumanogoh A.
    Nature Medicine (2017) 23: 1436-1443. [Pubmed]
  • Fv-clasp: An artificially designed small antibody fragment with improved production compatibility, stability, and crystallizability
  • Arimori, T, Kitago, Y, Umitsu, M, Fujii, Y, Asaki, R, Tamura-Kawakami, K, and Takagi, J.
    Structure (2017) 25: 1611-1622. [Pubmed]
  • Mechanistic basis for the recognition of laminin-511 by a6b1 integrin
  • Takizawa, M, Arimori, T, Taniguchi, Y, Kitago, Y, Yamashita, E, Takagi, J, and Sekiguchi, K.
    Science Advances (2017) 3(9): e1701497. [Pubmed]
  • Loss of Parkinson’s disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c
  • Meng H, Yamashita C, Shiba-Fukushima K, Inoshita T, Funayama M, Sato S, Hatta T, Natsume T, Umitsu M, Takagi J, Imai Y, Hattori N.
    Nature Commun. (2017) 8: 15500. [Pubmed]
  • Structural basis for perception of diverse chemical substances by T1r taste receptors
  • Nuemket N, Yasui N, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko M.K, Takagi J, Hosotani M, Yamashita A.
    Nature Commun. (2017) 8: 15530. [Pubmed]
  • Structural basis for ligand capture and release by the endocytic receptor ApoER2
  • Hirai H, Yasui N, Yamashita K, Tabata S, Yamamoto M, Takagi J, Nogi T.
    EMBO Rep. (2017) pii: e201643521. [Pubmed]
  • Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation
  • Shirakabe K, Omura T, Shibagaki Y, Mihara E, Homma K, Kato Y, Yoshimura A, Murakami Y, Takagi J, Hattori S, Ogawa Y.
    Scientific Rep. (2017) 7:46174. [Pubmed]
  • The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2
  • Fukuda S, Kita S, Obata Y, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nishizawa H, Funahashi T, Takagi J, Maeda N, and Shimomura I.
    J Biol Chem. (2017) 292 (19), 7840-7849. [Pubmed]
  • 2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching
  • Matsumoto A, Miyazaki N, Takagi J, and Iwasaki K.
    Scientific Rep. (2017) 7(1):377. [Pubmed]
  • Conformational freedom of the LRP6 ectodomain is regulated by N-glycosylation and the binding of the Wnt antagonist Dkk1
  • Matoba K, Mihara E, Tamura-Kawakami K, Miyazaki N, Maeda S, Hirai H, Thompson S, Iwasaki K, and Takagi J.
    Cell Reports (2017) 18, 32-40. [Pubmed]
  • [2016]
  • Allosteric inhibition of a semaphorin 4D receptor plexin B1 by a high-affinity macrocyclic peptide
  • Matsunaga Y, Bashiruddin NK, Kitago Y, Takagi J* and Suga H.
    Cell Chem. Biol. (2016) 23:1341-1350. [Pubmed]
  • Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies
  • Umitsu M, Ogasawara S, Sakai K, Kaneko M, Asaki R, Tamura-Kawakami K, Kato Y, Matsumoto K, and Takagi J.
    Scientific Rep. (2016) 9 (6):33149. [Pubmed]
  • Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by ERdj5
  • Ushioda R, Miyamoto A, Inoue M, Watanabe S, Okumura M, Maegawa K, Uegaki K, Fujii S, Fukuda Y, Umitsu M, Takagi J, Inaba K, Mikoshiba K, and Nagata K.
    Proc. Natl. Acad. Sci. USA (2016) 113(41):E6055-E6063. [Pubmed]
  • Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1
  • Kitago Y, Kaneko MK, Ogasawara S, Kato Y, and Takagi J.
    Biochem Biophys Res Commun. (2016) 478(3):1274-9. [Pubmed]
  • Molecular Basis of the Ligand Binding Specificity of αvβ8 Integrin
  • Ozawa A, Sato Y, Imabayashi T, Uemura T, Takagi J, and Sekiguchi K.
    J Biol Chem. (2016) 291(22):11551-65. [Pubmed]
  • Structure of the plexin ectodomain bound by semaphorin-mimicking antibodies
  • Suzuki K, Tsunoda H, Omiya R, Matoba K, Baba T, Suzuki S, Segawa H, Kumanogoh A, Iwasaki K, Hattori K, Takagi J.
    PLoS One (2016) 11 (6):e0156719. [Pubmed]
  • Tailored placement of a turn-forming PA tag into the structured domain of a protein to probe its conformational state
  • Fujii Y, Matsunaga Y, Arimori T, Kitago Y, Ogasawara S, Kaneko M, Kato Y, and Takagi J.
    J. Cell Science (2016) 129:1512-1522. [Pubmed]
  • Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin
  • Mihara E, Hirai H, Yamamoto H, Tamura-Kawakami K, Matano M, Kikuchi A, Sato T, and Takagi J.
    eLife (2016) 5:e11621. [Pubmed]
  • Structural and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase
  • Morita J, Kano K, Kato K, Takita H, Skagami H, Yamamoto Y, Mihara E, Ueda H, Sato T, Tokuyama H, Arai H, Asou H, Takagi J, Ishitani R, Nishimasu H, Nureki O, and Aoki J.
    Scientific Rep. (2016) 6:20995. [Pubmed]
  • [2015]
  • Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration
  • Kharitidi D, Apaja PM, Manteghi S, Suzuki K, Malitskaya E, Roldan A, Gingras MC, Takagi J, Lukacs GL, AND Pause A.
    Cell Rep. (2015) 13(3):599-609. [Pubmed]
  • Structural basis for amyloidogenic peptide recognition by sorLA
  • Kitago Y, Nagae M, Nakata Z, Yagi-Utsumi M, Takagi-Niidome S, Mihara E, Nogi T, Kato K, AND Takagi J.
    Nature Struct. Mol. Biol. (2015) 22:199-206. [Pubmed]
  • [2014]
  • Editorial overview: New constructs and expression of proteins: Making things better
  • Takagi, J. and Tate, CG.
    Curr Opin Struct Biol. (2014) 26:iv-vi. [Pubmed]
  • Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6
  • Morita J. et al.
    Acta Crystallogr. F Struct. Biol. Commun. (2014) 70:794-799. [Pubmed]
  • PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin
  • Fujii, Y. et al.
    Protein Exp. Purif. (2014) 95:240-247. [Pubmed]
  • Lysosomal sorting of amyloid-β by the SORLA receptor is impaired by a familial Alzheimer’s disease mutation
  • Caglayan, S. et al.
    Science Transl. Med. (2014) 6:223ra20. [Pubmed]
  • A structure-based model of substrate discrimination by a non-canonical PDZ tandem in the intramembrane-cleaving protease RseP
  • Hizukuri, Y. et al.
    Structure (2014) 22:326-336. [Pubmed]
  • Arl6IP1 has the ability to shape the mammalian ER membrane in a reticulon-like fashion
  • Yamamoto, Y. et al.
    Biochem. J. (2014) 458:69-79. [Pubmed]
  • [2013]
  • Life cycle of phytoreoviruses visualized by electron microscopy and tomography
  • Miyazaki, N. et al.
    Front. Microbiol. (2013) 4:1-9. [Pubmed]
  • Cryo-electron tomography: moving towards revealing the viral life cycle of Rice dwarf virus
  • Miyazaki, N. et al.
    J. Synchrotron Radiat. (2013) 20:826-828. [Pubmed]
  • Crystal structure and enzymatic activity of an ADAMTS13 mutant with the East Asian-specific P475S polymorphism
  • Akiyama M. et al.
    J Thromb Haemost. (2013) 11:1399-1406. [Pubmed]
  • A multi-purpose fusion tag derived from an unstructured and hyper-acidic region of the amyloid precursor protein
  • Sangawa T. et al.
    Protein Sci. (2013) 22:840-850. [Pubmed]
  • A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration
  • Nojima S. et al.
    Nature Commun. (2013) 4:1406 [Pubmed]
  • [2012]
  • Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling
  • Kato K. et al.
    Proc. Natl. Acad. Sci. USA (2012) 109:16876-16881. [Pubmed]
  • Schwann Cell Myelination Requires Integration of Laminin Activities
  • McKee, K.K. et al.
    J. Cell Sci. (2012) 125:4609-4619. [Pubmed]
  • Higher-order architecture of cell adhesion mediated by polymorphic synaptic adhesion molecules neurexin and neuroligin
  • Tanaka, H. et al.
    Cell Reports (2012) 2:101-110. [Pubmed]
  • Gold Nanoparticle-Induced Formation of Artificial Protein Capsids
  • Malay, A.D. et al.
    Nano Lett (2012) 12:2056-2059. [Pubmed]
  • Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1
  • Kato K. et al.
    Acta Crystallogr. F (2012) 68:778-782. [Pubmed]
  • Crystal structure of α5β1 integrin ectodomain: Atomic details of the fibronectin receptor
  • Nagae, M. et al.
    J. Cell Biol. (2012) 197:131-140. [Pubmed]
  • Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer
  • Matoba K. et al.
    J Electron Microsc (Tokyo) (2012) 61:193-198. [Pubmed]
  • Neutralization of the g-secretase activity by monoclonal antibody against extracellular domain of nicastrin
  • Hayashi, I. et al.
    Oncogene (2012) 31:787-798. [Pubmed]
  • [2011]
  • Functional importance of covalent homodimer of reelin protein linked via its central region
  • Yasui, N. et al.
    J. Biol. Chem. (2011) 286:35247-35256. [Pubmed]
  • Structural Basis for Variant-Specific Neuroligin-Binding by α-Neurexin
  • Tanaka, H. et al.
    PLoS One (2011) 6:e19411[Pubmed]
  • Crystal structure of the cis-dimer of nectin-1: implications for the architecture of cell-cell junctions
  • Narita, H. et al.
    J.Biol.Chem. (2011) 286:12659-12669. [Pubmed]
  • Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure
  • Akita, F. et al.
    J. Gen. Virol. (2011) 92:2214-2221. [Pubmed]
  • Three-dimensional analysis of the association of viral particles with mitochondria during the replication of Rice gall dwarf virus
  • Wei, T. et al.
    J. Mol. Biol. (2011) 410(3):436-446. [Pubmed]
  • Crystal structure of Autotaxin, a secreted lysophospholipase D that generates GPCR-signaling lipid mediator
  • Nishimasu, H. et al.
    Nature Struct. Mol. Biol. (2011) 18:205-212. [Pubmed]
  • Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain
  • Nakata, Z. et al.
    Acta Crystallographica. (2011) F67:129-132. [Pubmed]
  • [2010]
  • Electron tomography of the supramolecular structure of virus-infected cells
  • Iwasaki, K. and Omura, T.
    Curr.Opin.Struct.Biol. (2010) 50:632-639. [Pubmed]
  • Tomosyn Inhibits Synaptotagmin-1-mediated Step of Ca2+-dependent Neurotransmitter Release through Its N-terminal WD40 Repeats
  • Yamamoto, Y. et al.
    J. Biol. Chem. (2010) 285:40943-40955. [Pubmed]
  • Structural basis for semaphorin signaling through the plexin receptor
  • Nogi, T. et al.
    Nature (2010) 467:1123-1127. [PubMed]
  • Comparison of the envelope architecture of E. coli using two methods: CEMOVIS and cryo-electron tomography
  • Kishimoto-Okada, A. et al.
    J. Electron Microsc (2010) 59:419-426. [PubMed]
  • Laminin-121-Recombinant expression and interactions with integrins
  • Sasaki, T. et al.
    Matrix Biology (2010) 29:484-493. [PubMed]
  • A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system
  • Tabata, S. et al.
    J. Proteomics. (2010) 73:1777-1785. [PubMed]
  • Structural basis for specific recognition of reelin by its receptors
  • Yasui, N. et al.
    Structure (2010) 18:320-331.[PubMed]
  • Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation
  • Yasui, N. et al.
    J. Immunol. Methods (2010) 352:153-160. [PubMed]
  • [2009]
  • The structure of rat liver vault at 3.5 angstrom resolution
  • Tanaka, H. et al.
    Science (2009) 323:384-388. [PubMed]
  • Molecular dynamism of Fe-S cluster biosynthesis implicated by the structure of SufC2-SufD2 complex
  • Wada, K. et al.
    J. Mol. Biol. (2009) 387:245-258. [PubMed]
  • Molecular basis of the recognition of nephronectin by integrin alpha8beta1
  • Sato, Y. et al.
    J. Biol. Chem. (2009) 284:14524-14536. [PubMed]
  • Production, crystallization and preliminary crystallographic analysis of the exosite-containing fragment of human von Willebrand factor-cleaving proteinase, ADAMTS13
  • Akiyama, M. et al.
    Acta Crystallographica (2009) F65:739-742. [PubMed]
  • Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor
  • Akiyama, M. et al.
    Proc. Natl. Acad. Sci. USA (2009) 106:19274-19279.[PubMed]
  • A self-assembled protein nanotube with high aspect ratio
  • Miranda, F. et al.
    Small (2009) 5:2077-2084. [PubMed]
  • Association of Rice gall dwarf virus with microtubules is necessary for viral release from cultured insect vector cells
  • Wei, T., et al.
    J. Virol. (2009) 83:10830-10835. [PubMed]
  • [2008]
  • Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus
  • Yamaguchi, H. et al.
    J. Leukocyte Biol. (2008) 83:1300-1307. [PubMed]
  • Key interactions in ectodomain of integrin for global conformational change detected by elastic network normal mode analysis
  • Matsumoto, A. et al.
    Biophys. J. (2008) 95(6):2895-2908. [PubMed]
  • Structure of F-spondin reeler domain reveals a unique b-sandwich fold with a deformable disulfide-bonded loop
  • Nagae, M. et al.
    Acta Crystallographica. (2008) D64:1138-1145. [PubMed]
  • A murine monoclonal antibody that binds N-terminal extracellular segment of human protease activated receptor-4
  • Sangawa, T. et al.
    Hybridoma (2008) 27(5):331-335. [PubMed]
  • Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification
  • Nogi, T. et al.
    Protein Sci. (2008) 17:2120-2126. [PubMed]
  • A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
  • Kato, K. et al.
    Acta Crystallogr. (2008) D64:525-531. [PubMed]
  • Pleomorphic Configuration of the Trimeric Capsid Proteins of Rice dwarf virus that Allows Formation of Both the Outer Capsid and Tubular Crystals
  • Iwasaki, K. et al.
    J.Mol.Biol. (2008) 383:252-265. [PubMed]
  • [2007]
  • Structural basis for ligand recognition by integrins
  • Takagi, J.
    Curr. Opin. Cell Biol. (2007) 19:557-564.[PubMed]
  • Structure of a receptor-binding reelin fragment and mutational analysis reveal a recognition mechanism similar to endocytic receptors
  • Yasui, N. et al.
    Proc. Natl. Acad. Sci. USA (2007) 104:9988-9993.[PubMed]
  • The RGD motif in fibronectin is essential for mouse development but dispensable for fibronectin fibril assembly
  • Takahashi, S. et al.
    J. Cell Biol. (2007) 178:167-178. [PubMed]
  • Three-dimensional architecture of virus-packed tubule
  • Katayama, S. et al.
    J. Electron Microsc. (2007) 56:77-81. [PubMed]
  • Semaphorin 7A initiates T cell-mediated inflammatory responses through alpha1beta1 integrin
  • Suzuki K. et al.
    Nature (2007) 446:680-684. [PubMed]
  • [2006]
  • alphaVbeta6 is a novel receptor for human fibrillin-1: comparative studies of molecular determinants underlying integrin-RGD affinity and specificity
  • Jovanovic, J. et al.
    J. Biol. Chem. (2006) 282:6743-6751. [PubMed]
  • Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography
  • Nogi, T. et al.
    EMBO J. (2006) 25:3675-3683. [PubMed]
  • Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation
  • Tarui, T. et al.
    Thromb Haemost. (2006) 95:524-534. [PubMed]
  • Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins
  • Nishiuchi, R. et al.
    Matrix Biol. (2006) 25:189-197.[PubMed]
  • [2005]
  • Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering
  • Luo, B. H. et al.
    Proc. Natl. Acad. Sci. USA (2005) 102:3679-3684. [PubMed]
  • Potentiation of the ligand-binding activity of integrin alpha3 beta1 via association with tetraspanin CD151
  • Nishiuchi, R. et al.
    Proc. Natl. Acad. Sci. USA (2005) 102:1939-1944. [PubMed]

☆別刷り希望の方はメールで。

【和文総説】

  • 37. 菅野(中村)希、高木淳一 (2021) 環状ペプチドとタンパク質の融合による新しいバイオ医薬品モダリティの創成(特集 ペプチド化学の進歩と次世代の創薬)、 ファルマシア, 57(9), 820-824. [link]
  • 36. 星野温、有森貴夫 (2021) 指向性進化法による高親和性ACE2創出とCOVID-19創薬展開、 医学のあゆみ, 278, 588-595. [link]
  • 35. 有森貴夫 (2021) タンパク質結晶化に応用可能な新規小型抗体フォーマットFv-claspの開発、 日本結晶学会誌, 63, 113-120. [link]
  • 34. 有森貴夫、高木淳一 (2018) 小型抗体の作製技術(特集 次世代抗体医薬の衝撃)、実験医学、36(11), 1849-1853. [link]
  • 33. 有森貴夫、高木淳一 (2018) 新規小型抗体「Fv-clasp」の開発、バイオサイエンスとバイオインダストリー、76(2), 132-133. [link]
  • 32. 的場京子、高木淳一 (2017) 糖鎖によってゆらぐ受容体蛋白質の構造を観る、医学のあゆみ、262(5), 424-430. [link]
  • 31. 高木淳一 (2016) PAタグ:ループ挿入可能な超高親和性ペプチドタグ、生物物理、56(5), 284-287. [link]
  • 30. 海津正賢、高木淳一 (2016) HGF-Metシグナル伝達の構造的基盤、日本血栓止血学会誌、27(1), 77-84. [link]
  • 29. 北郷 悠、高木淳一 (2015) 巨大受容体sorLAによるアミロイドβ分解の促進、医学のあゆみ、252(9), 963-964. [link]
  • 28. 海津正賢、高木淳一 (2015) 構造生物学が明らかにする細胞膜を介したシグナリング機構、実験医学、33(10), 183-189. [link]
  • 27. 禾 晃和、三原恵美子、高木淳一 (2013) 動物細胞発現系を用いた試料調製、「タンパク質結晶の最前線」(杉山 成 監修)シーエムシー出版、 [link]
  • 26. 岩崎憲治 (2013) 電子線トモグラフィー、「膜タンパク質構造研究」(岩田 想 編)化学同人, pp 180-186 [link]
  • 25. 高木淳一 (2013) 動物細胞を用いる膜タンパク質生産、「膜タンパク質構造研究」(岩田 想 編)化学同人、 pp 103-110. [link]
  • 24. 高木淳一 (2013) 相関構造解析法による階層をまたがった生命機能の統合的解明、パリティ、28-07, 20-24.
  • 23. 宮崎直幸、田中宏樹、禾 晃和、岩崎憲治、高木淳一 (2013) 光-電子相関顕微鏡法~シナプス接着メカニズム解明への応用~、生物物理、53, 218-221. [link]
  • 22. 松永幸子、高木淳一 (2013) セマフォリン関連分子群の立体構造、実験医学、31(4), 523-530. [link]
  • 21. 北郷 悠、高木淳一 (2012) LDL受容体ファミリーの構造と蛋白質間相互作用、The Lipid、23(4), 56-62.
  • 20. 寒川 剛、高木淳一 (2011) タンパク質間相互作用の検出-表面プラズモン共鳴法、「タンパク質実験ノート(下)」(岡田雅人、三木裕明、宮崎 香 編)羊土社, pp 132-137. [link]
  • 19. 禾 晃和、高木淳一(2011) 神経軸索の伸長を司るガイダンス因子セマフォリンのシグナル伝達における過渡的複合体形成、生化学、83, 938-948. [link]
  • 18. 高木淳一 (2011) 動物細胞を用いる高難度タンパク質生産と迅速精製技術、BIO INDUSTRY、 28 (3), 7-13. [link]
  • 17. 秋山正志、武田壮一、小亀浩市、高木淳一、宮田敏行(2010) VWF切断酵素ADAMTS13のエキソサイト認識機構、生化学、82, 950-956 [link]
  • pub16img 16. 高木淳一 (2009) 微量蛋白質の1段階精製を可能にするTARGETタグシステム  「融合発展する構造生物学とケミカルバイオロジーの最前線」(長野哲雄、若槻壮市、高木淳一、古谷利夫 編)、共立出版、1468-1475. [Link] (表紙には当研究室で得た抗原-抗体の構造が使われています)[サンプル]


  • pub15img 15. 高木淳一 (2009) タンパク質の分子間相互作用の解析、「やさしい原理からはいるタンパク質科学実験法3-タンパク質の働きを知る-分子機能と生体作用」(長谷俊治、高尾敏文、高木淳一 編)、化学同人、29-52. [Link] (表紙には当研究室で得られた画像が使われています)






  • pub14img 14. 岩崎憲治 (2009) タンパク質の電子顕微鏡観察、「やさしい原理からはいるタンパク質科学実験法2-タンパク質をみる-構造と挙動」(長谷俊治・高尾敏文・高木淳一編)、化学同人、197-225. [Link] (表紙には当研究室で得られた画像が使われています)


  • 13. 岩崎憲治 (2009) M36生物学における話題と電子顕微鏡、日本結晶学会誌、51, 70-71. [Link]
  • 12. 高木淳一 (2009) インテグリン、「ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術」(田畑泰彦 編)、メディカルドゥ、 251-256. [Link]
  • 11. 宮崎直幸、岩崎憲治、大村敏博 (2009) クライオ電子顕微鏡単粒子解析法を用いたウイルス構造解析、 Virus Report、医薬ジャーナル社、6(1), 52-61. [Link]
  • 10. 岩崎憲治、宮崎直幸、片山寿美枝、大村敏博 (2009) イネ委縮ウイルスのライフサイクルにおける超分子複合体の解析、顕微鏡、44(2), 82-86. [Link]
  • 9. 安井典久、禾晃和、高木淳一 (2008) 脳の層構造形成を司る細胞外タンパク質リーリンの構造生物学、生物物理、48, 235-238. [Link]
  • 8. 高木淳一(2007) 細胞接着分子とその受容体:細胞が細胞外マトリックスを識別する仕組み、「再生医療のための細胞生物学」(関口清俊 編)コロナ社, pp 26-48.
  • 7. 高木淳一 (2006)モジュラータンパク質の構造解析:受容体構造生物学における中心課題、日本結晶学会誌、48, 411-417. [Link]
  • 6. 高木淳一 (2005) 血小板GPIIb/IIIa複合体の立体構造、血液疾患-state of the arts-、pp.116-119.
  • 5. 高木淳一 (2005) レセプター膜貫通ドメインの構造とそのシグナリングにおける動的制御、蛋白質核酸酵素、50(10), 1226-1232.
  • 4. 高木淳一 (2005) 血小板インテグリンαIIbβ3の立体構造から解くリガンド認識の構造的基盤、日本血栓止血学会誌、16(1), 39-46 [Link]
  • 3. 岩崎憲治、高木淳一 (2005) 電子顕微鏡を用いた蛋白質の高次構造解析が開く世界-インテグリンレセプターの構造解析を中心として-、生化学、77,400-410 [Link]
  • 2. 岩崎憲治、宮澤淳夫 (2005) クライオ電子顕微鏡法とX線結晶構造解析のハイブリッドアプローチによって提案されたイノシトール3リン酸受容体のCa2+放出のメカニズム. 生物物理, 45,192-197 [Link]
  • 1. 岩崎憲治(2005) 細胞内の分子を電子顕微鏡で見る、Bionics(バイオニクス)11月号,オーム社p.p.38-43. [Link]

蛋白質研究所  分子創製学研究室

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