蛋白質研究所分子創製学 / 業績・高木

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PUBLICATION LIST for J. TAKAGI（高木淳一）May 2025

248) Ito T, Suzuki T, Sakai Y, Nishioka K, Itoh Y, Sakamoto K, Ikemura N, Matoba S, Kanda Y, Takagi J, Okamoto T, Tahara K, Hoshino A. (2025) Engineered ACE2 Decoy in Dry Powder Form for Inhalation: A Novel Therapy for SARS-CoV-2 Variants. Ther. Methods & Clin. Dev., 33(2):101459.
247) Kobiyama K, Utsumi D, Kaku Y, Sasaki E, Yasui F, Okamura T, Onodera T, Tobuse AJ, Sakkour A, Amiry AF, Hayashi T, Temizoz B, Liu K, Negishi H, Toyama-Sorimachi N, Kohara M, Sawasaki T, Takagi J, Sato K, Takahashi Y, Yasutomi Y, Ishii KJ. (2025) Immunological analysis of LC16m8 vaccine: preclinical and early clinical insights into mpox. eBioMedicine, 115:105703.
246) Ikeda S, Hasegawa K, Kogue Y, Arimori T, Kawamoto R, Wibowo T, Yaga M, Inada Y, Uehara H, Matsubara M, Tachikawa M, Suga M, Kida S, Shibata K, Tsutsumi K, Fukushima K, Fujita J, Ueda T, Kusakabe S, Hino A, Ichii M, Hirose A, Nakamae H, Hino M, Nakao T, Inoue M, Yoshihara K, Yoshihara S, Ueda S, Tachi T, Kuroda H, Murakami K, Kijima N, Kishima H, Igashira E, Murakami M, Takiuchi T, Kimura T, Hiroshima T, Kimura T, Shintani Y, Imai C, Yusa K, Mori R, Ogino T, Eguchi H, Takeda K, Oji Y, Kumanogoh A, Takagi J, Hosen N. (2025) CAR T or NK cells targeting mismatched HLA-DR molecules in acute myeloid leukemia after allogeneic hematopoietic stem cell transplant. Nat. Cancer, 6(4):595-611.
245) Chida S, Chiba T, Uchida Y, Matsushima T, Kurimoto R, Miyazaki T, Yagasaki L, Nakamura S, Mihara E, Takagi J, Moriyama K, Asahara H. (2025) Impact of mechanotransduction on gene expression changes in periodontal ligament during orthodontic tooth movement. J. Bone Miner. Metab., in press
244) Takahashi K, Kiso H, Mihara E, Takagi J, Tokita Y, Murashima-Suginami A. (2024) Development of a new antibody drug to treat congenital tooth agenesis. J. Oral Biosci., 66(4):1-9.
243) Rojas-Chaverra NM, Imamura R, Sato H, Passioura T, Mihara E, Nishimura T, Takagi J, Suga H, Matsumoto K, Sakai K. (2024) A cyclic peptide-grafted Fc with hepatocyte growth factor functionality ameliorates hepatic fibrosis in a non-alcoholic steatohepatitis mouse model. iScience, 27(8):110426.
242) Franzolin G, Brundu S, Cojocaru CF, Curatolo A, Ponzo M, Mastrantonio R, Mihara E, Kumanogoh A, Suga H, Takagi J, Tamagnone L, Giraudo E. (2024) PlexinB1 Inactivation Reprograms Immune Cells in the Tumor Microenvironment, Inhibiting Breast Cancer Growth and Metastatic Dissemination. Cancer Immunol Res., 12(9):1286-1301.
241) Arimori T, Mihara E, Suzuki H, Ohishi T, Tanaka T, Kaneko MK, Takagi J, Kato Y. (2024) Locally misfolded HER2 expressed on cancer cells is a promising target for development of cancer-specific antibodies. Structure, 32(5):536-549.
240) Hua Z, Watanabe R, Fukunaga T, Matsui Y, Matsuoka M, Yamaguchi S, Tanabe S, Yamamoto M, Kawakami KT, Takagi J, Kajita M, Futai E, Shirakabe K. (2024) C-terminal amino acids in the type I transmembrane domain of L-type lectin VIP36 affect γ-secretase susceptibility. Biochem Biophys Res Commun., 12:696:149504.
239) Arase N, Sasaoka Y, Narita J, Kiyohara E, Hashimoto K, Shinzaki S, Nojima S, Takagi J, Fujimoto M. (2024) Anti-α6β4 integrin autoantibodies inhibit the binding of laminins to α6β4 integrin in patients with pemphigoid and affect the gastrointestinal tract. J Eur Acad Dermatol Venereol., 38(2):404-412.
238) 有森貴夫，高木淳一 (2024) 次世代型Fc融合法による超効率的な疾病関連蛋白質の生産、実験医学、 43(2):82-88.
237) Urano E, Itoh Y, Suzuki T, Sasaki T, Kishikawa J, Akamatsu K, Higuchi Y, Sakai Y, Okamura T, Mitoma S, Sugihara F, Takada A, Kimura M, Nakao S, Hirose M, Sasaki T, Koketsu R, Tsuji S, Yanagida S, Shioda T, Hara E, Matoba S, Matsuura Y, Kanda Y, Arase H, Okada M, Takagi J, Kato T, Hoshino A, Yasutomi Y, Saito A, Okamoto T. (2023) An inhaled ACE2 decoy confers protection against SARS-CoV-2 infection in preclinical models. Sci Transl Med., 15(711):eadi2623.
236) Shirai T, Nakai A, Ando E, Fujimoto J, Leach S, Arimori T, Higo D, Eerden F, Tulyeu J, Liu Y, Okuzaki D, Murayama MA, Miyata H, Nunomura K, Lin B, Tani A, Kumanogoh A, Ikawa M, Wing JB, Standley DM, Takagi J, Suzuki K. (2023) Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex. Science Immunology, 8(81):eadc9324.
235) Kikuchi A, Takagi J, Takada S, Ishitani T, Minami Y. (2023) Wnt 2022 EMBO | the Company of Biologists workshop and Yamada conference. Genes to Cells, 28(4):249-257.
234) Kuhn CC, Basnet N, Bodakuntla S, Alvarez-Brecht P, Nichols S, Martinez-Sanchez A, Agostini L, Soh YM, Takagi J, Biertümpfel C, Mizuno N. (2023) Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein. Nat Commun., 14(1):620.
233) Ravi V, Murashima-Suginami A, Kiso H, Tokita Y, Huang CL, Bessho K, Takagi J, Sugai M, Tabata Y, Takahashi K. (2023) Advances in tooth agenesis and tooth regeneration. Regen Ther., 3:22:160-168.
232) Sakai K, Sugano-Nakamura N, Mihara E, Rojas-Chaverra NM, Watanabe S, Sato H, Imamura R, Voon DC, Sakai I, Yamasaki C, Tateno C, Shibata M, Suga H, Takagi J, Matsumoto K. (2022) Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions. Nat Biomed Eng., 7(2):164-176.
231) Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M, Kiuchi T, Takagi J, Watanabe N. (2022) Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Reports Methods, 2(10):100301.
230) Sen S, Spasic A, Sinha A, Wang J, Bush M, Li J, Nešić D, Zhou Y, Angiulli G, Morgan P, Salas-Estrada L, Takagi J, Walz T, Coller BS, Filizola M. (2022) Structure-Based Discovery of a Novel Class of Small-Molecule Pure Antagonists of Integrin αVβ3. J Chem Inf Model., 62(22):5607-5621.
229) Tanaka T, Ekimoto T, Nagatomo M, Neyazaki M, Shimoji E, Yamane T, Kanagawa S, Oi R, Mihara E, Takagi J, Akashi S, Ikeguchi M, Nogi T. (2022) Hybrid in vitro/in silico analysis of low-affinity protein-protein interactions that regulate signal transduction by Sema6D. Protein Sci., 31(11):e4452.
228) Sugano-Nakamura N, Matoba K, Hirose M, Bashiruddin NK, Matsunaga Y, Yamashita K, Hirata K, Yamamoto M, Arimori T, Suga H, Takagi J. (2022) De novo Fc-based receptor dimerizers differentially modulate PlexinB1 function. Structure, 30(10):1411-1423.e4.
227) Arimori T, Ikemura N, Okamoto T, Takagi J, Standley DM, Hoshino A. (2022) Engineering ACE2 decoy receptors to combat viral escapability.Trends Pharmacol Sci., 43(10):838-851.
226) Ko SB, Mihara E, Park Y, Roh K, Kang C, Takagi J, Bang I, Choi HJ. (2022) Functional role of the Frizzled linker domain in the Wnt signaling pathway.Commun Biol., 5(1):421.
225) Ikemura N, Taminishi S, Inaba T, Arimori T, Motooka D, Katoh K, Kirita Y, Higuchi Y, Li S, Suzuki T, Itoh Y, Ozaki Y, Nakamura S, Matoba S, Standley DM, Okamoto T, Takagi J, Hoshino A. (2022) An engineered ACE2 decoy neutralizes the SARS-CoV-2 Omicron variant and confers protection against infection in vivo. Sci Transl Med., eabn7737.
224) Hasegawa K, Ikeda S, Yaga M, Watanabe K, Urakawa R, Iehara A, Iwai M, Hashiguchi S, Morimoto S, Fujiki F, Nakajima H, Nakata J, Nishida S, Tsuboi A, Oka Y, Yoshihara S, Manabe M, Ichihara H, Mugitani A, Aoyama Y, Nakao T, Hirose A, Hino M, Ueda S, Takenaka K, Masuko T, Akashi K, Maruno T, Uchiyama S, Takamatsu S, Wada N, Morii E, Nagamori S, Motooka D, Kanai Y, Oji Y, Nakagawa T, Kijima N, Kishima H, Ikeda A, Ogino T, Shintani Y, Kubo T, Mihara E, Yusa K, Sugiyama H, Takagi J, Miyoshi E, Kumanogoh A, Hosen N. (2022) Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain. Sci Transl Med., 14(632):eaax7706.
223) Yamashita C, Arase N, Higuchi S, Arase H, Takagi J, Nojima S, Tanemura A, Fujimoto M. (2021) Serum autoantibodies against the extracellular region of α6β4 integrin in a patient with dipeptidyl peptidase-4 inhibitor-induced bullous pemphigoid. JAAD Case Rep., 20:65-68.
222) 菅野（中村）希、高木淳一 (2021) 環状ペプチドとタンパク質の融合による新しいバイオ医薬品モダリティの創成（特集　ペプチド化学の進歩と次世代の創薬）、ファルマシア、57(9):820-824.
221) Komatsu Y, Terasaka N, Sakai K, Mihara E, Wakabayashi R, Matsumoto K, Hilvert D, Takagi J, Suga H. (2021) De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist. iScience, 24(11):103302.
220) Arimori T, Miyazaki N, Mihara E, Takizawa M, Taniguchi Y, Cabañas C, Sekiguchi K, Takagi J. (2021) Structural mechanism of laminin recognition by integrin. Nat. Commun., 12(1): 4012.
219) Higuchi Y, Suzuki T, Arimori T, Ikemura N, Mihara E, Kirita Y, Ohgitani E, Mazda O, Motooka D, Nakamura S, Sakai Y, Itoh Y, Sugihara F, Matsuura Y, Matoba S, Okamoto T, Takagi J, Hoshino A. (2021) Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2. Nat. Commun., 12(1):3802.
218) Schumacher S, Dedden D, Nunez RV, Matoba K, Takagi J, Biertümpfel C, Mizuno N. (2021) Structural insights into integrin α5β1 opening by fibronectin ligand. Sci. Adv., 7(19):eabe9716.
217) Kobashigawa Y, Ohara T, Morita K, Toyota Y, Nakamura T, Kotani S, Arimori T, Yamauchi S, Liu C, Kitazaki M, Wakeyama-Miyazaki Y, Suwa Y, Uchida-Kamekura M, Fukuda N, Sato T, Nakajima M, Takagi J, Yamagata Y, Morioka H. (2021) Molecular recognition of a single-chain Fv antibody specific for GA-pyridine, an advanced glycation end-product (AGE), elucidated using biophysical techniques and synthetic antigen analogues. J. Biochem., 170(3):379-387.
216) Mihara E, Watanabe S, Bashiruddin NK, Nakamura N, Matoba K, Sano Y, Maini R, Yin Y, Sakai K, Arimori T, Matsumoto K, Suga H, Takagi J. (2021) Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins. Nat. Commun., 12(1):1543.
215) Murashima-Suginami A, Kiso H, Tokita Y, Mihara E, Nambu Y, Uozumi R, Tabata Y, Bessho K, Takagi J, Sugai M, Takahashi K. (2021) Anti-USAG-1 therapy for tooth regeneration through enhanced BMP signaling. Sci. Adv., 7(7):eabf1798.
214) Sato N, Yogo R, Yanaka S, Martel A, Porcar L, Morishima K, Inoue R, Tominaga T, Arimori T, Takagi J, Sugiyama M, Kato K. (2021) A feasibility study of inverse contrast-matching small-angle neutron scattering method combined with size exclusion chromatography using antibody interactions as model systems. J. Biochem., 169(6):701-708.
213) Nagae M, Suzuki K, Yasui N, Nogi T, Kohno T, Hattori M, Takagi J. (2020) Structural studies of reelin N-terminal region provides insights into a unique structural arrangement and functional multimerization. J. Biochem. 169(5):555-564.
212) Bashiruddin NK, Hayashi M, Nagano M, Wu Y, Matsunaga Y, Takagi J, Nakashima T, Suga H. (2020) Development of cyclic peptides with potent in vivo osteogenic activity through RaPID-based affinity maturation. Proc. Natl. Acad. Sci. U S A., 117(49):31070-31077.
211) Sato T, Ishihara S, Marui R, Takagi J, Katagiri K. (2020) Dissection of α4β7 integrin regulation by Rap1 using novel conformation-specific monoclonal anti-β7 antibodies. Sci. Rep., 10(1):13221.
210) Iwagishi R, Tanaka R, Seto M, Takagi T, Norioka N, Ueyama T, Kawamura T, Takagi J, Ogawa Y, and Shirakabe K. (2020) Negatively charged amino acids in the stalk region of membrane proteins reduce ectodomain shedding. J. Biol. Chem. 295(35):12343-12352.
209) Wakasa A, Kaneko MK, Kato Y, Takagi J, and Arimori T. (2020) Site-specific epitope insertion into recombinant proteins using the MAP tag system. J. Biochem., 168(4):375-384.
208) Otero-Ramirez ME, Matoba K, Mihara E, Passioura T, Takagi J, and Suga H. (2020) Macrocyclic Peptides that Inhibit Wnt Signalling via interaction with Wnt3a. RSC Chemical Biol. 1, 26-34.
207) Umitsu M, Sakai K, Tamura-Kawakami K, Matsumoto K, Takagi J. (2020) The constitutive high affinity Met binding site in the kringle domain is dispensable for the signaling activity of hepatocyte growth factor. J Biochem. 167(6):577-586.
206) Li J, Fukase Y, Shang Y, Zou W, Muñoz-Félix JM, Buitrago L, van Agthoven J, Zhang Y, Hara R, Tanaka Y, Okamoto R, Yasui T, Nakahata T, Imaeda T, Aso K, Zhou Y, Locuson C, Nesic D, Duggan M, Takagi J, Vaughan RD, Walz T, Hodivala-Dilke K, Teitelbaum SL, Arnaout MA, Filizola M, Foley MA, Coller BS. (2019) Novel Pure αVβ3 Antagonists That Do Not Induce Receptor Extension, Prime the Receptor, or Enhance Angiogenesis at Low Concentrations. ACS Pharmacol. Transl. Sci 2, 387-401.
205) Miao W, Sakai K, Sato H, Imamura R, Jangphattananont N, Takagi J, Nishita M, Minami Y, Matsumoto K. (2019) Impaired ligand-dependent MET activation caused by an extracellular SEMA domain missense mutation in lung cancer. Cancer Sci. 110(10): 3340-3349.
204) Jangphattananont N, Sato H, Imamura R, Sakai K, Terakado Y, Murakami K, Barker N, Oshima H, Oshima M, Takagi J, Kato Y, Yano S, Matsumoto K. (2019) Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach. Int J Mol Sci. 20(12). pii: E2955.
203) Sakai K, Passioura T, Sato H, Ito K, Furuhashi H, Umitsu M, Takagi J, Kato Y, Yano S, Shibata M, Suga H*, and Matsumoto K* (2019) Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor. Nature Chem. Biol. 15(6):598-606.
202) Hirai H, Matoba K, Mihara E, Arimori T, and Takagi J* (2019) Crystal structure of mammalian Wnt–frizzled complex. Nature Struct. Mol. Biol. 26(5):372-379.
201) Inoue M, Sakuta N, Watanabe S, Yoshikaie K, Tanaka Y, Ushioda R, Kato Y, Takagi J, Tsukazaki T, Nagata K, and Inaba K* (2019) Structural basis of sarco/endoplasmic reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay. Cell Rep. 27(4):1221-1230.e3.
200) Nagamura R, Fukuda M, Kawamoto A, Matoba K, Naoshi Dohmae N, Ishitani R, Takagi J, and Nureki O (2019) Structural basis for oligomerization of the prokaryotic peptide transporter PepTSo2. Acta Crystallogr. F, 75(Pt 5):348-358.
199) Brown ZP, Takagi J. (2019) Advances in domain and subunit localization technology for electron microscopy. Biophys Rev. 11(2):149-155.
198) Brown ZP, Takagi J. (2018) The PA tag: A versatile peptide tagging system in the era of integrative structural biology. Adv Exp Med Biol. 1105:59-76.
197) Miao W, Sakai K, Ozawa N, Nishiuchi T, Suzuki Y, Ito K, Morioka T, Umitsu M, Takagi J, Suga H, Matsumoto K. (2018) Cellular signaling and gene expression profiles evoked by a bivalent macrocyclic peptide that serves as an artificial MET receptor agonist. Sci Rep. 8(1):16492.
196) Tsunoda J, Song C, Imai FL, Takagi J, Ueno H, Murata T, Iino R, Murata K. (2018) Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy. Sci Rep. 8(1):15632.
195) Nagae M, Kizuka Y, Mihara E, Kitago Y, Hanashima S, Ito Y, Takagi J, Taniguchi N, Yamaguchi Y. (2018) Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V. Nat Commun., 9(1): 3380.
194) 有森貴夫、高木淳一 (2018) 小型抗体の作製技術（特集　次世代抗体医薬の衝撃）、実験医学、36(11): 1849-1853.
193) Kang S, Nakanishi Y, Kioi Y, Okuzaki D, Kimura T, Takamatsu H, Koyama S, Nojima S, Nishide M, Hayama Y, Kinehara Y, Kato Y, Nakatani T, Shimogori T, Takagi J, Toyofuku T, and Kumanogoh A. (2018) Semaphorin 6D reverse signaling controls macrophage lipid metabolism and anti-inflammatory polarization. Nature Immunology, 19 (6): 561-570.
192) Bashiruddin N, Matsunaga Y, Nagano M, Takagi J, Suga H. (2018) Facile synthesis of dimeric thioether-macrocyclic peptides with antibody-like affinity against Plexin-B1. Bioconjug Chem. 29(6): 1847-1851.
191) 有森貴夫、高木淳一 (2018) 新規小型抗体「Fv-clasp」の開発、バイオサイエンスとバイオインダストリー、76(2): 132-133.
190) Miyazaki N, Iwasaki K, and Takagi J. (2018) Systematic survey of conformational states in β1 and β4 integrins by negative-stain electron microscopy. J. Cell Sci., 131(10): jcs.216754.
189) Wang H, Han W, Takagi J, Cong Y. (2018) Yeast inner-subunit PA-NZ-1 labeling strategy for accurate subunit identification in a macromolecular complex through cryo-EM analysis. J. Mol. Biol. 430 (10): 1417-1425.
188) Hall D, Takagi J, Nakamura H. (2018) Foreword to 'Multiscale structural biology: biophysical principles and mechanisms underlying the action of bio-nanomachines', a special issue in Honour of Fumio Arisaka's 70th birthday. Biophys. Rev. 10(2):105-129.
187) Tabata S, Kitago Y, Fujii Y, Mihara E, Tamura-Kawakami K, Norioka N, Takahashi K, Kaneko MK, Kato Y, and Takagi J. (2018) An anti-peptide monoclonal antibody recognizing the tobacco etch virus protease-cleavage sequence and its application to a tandem tagging system. Protein Expr Purif. 147: 94-99.
186) Seino T, Kawasaki S, Shimokawa M, Tamagawa H, Toshimitsu K, Fujii M, Ohta Y, Matano M, Nanki K, Kawasaki K, Takahashi S, Sugimoto S, Iwasaki E, Takagi J, Itoi T, Kitago M, Kitagawa Y, Kanai T, and Sato T. (2018) Human pancreatic tumor organoids reveal loss of stem cell niche factor dependence during disease progression. Cell Stem Cell, 22(3): 454-467.
185) Brown ZP, Arimori T, Iwasaki K, and Takagi J. (2018) Development of a new protein labeling system to map subunits and domains of macromolecular complexes for electron microscopy. J. Struct. Biol., 201, 247-251.
184) Sakakibara S, Arimori T, Yamashita K, Jinzai H, Motooka D, Nakamura S, Li S, Takeda K, Katayama J, Hussien MAE, Narazaki M, Tanaka T, Standley D, Takagi J, and Kikutani H. (2017) Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus. Scientific Rep. 7 (1), 16428.
183) Saito F, Hirayasu K, Satoh T, Wang CW3, Lusingu J, Arimori T, Shida K, Palacpac NMQ, Itagaki S, Iwanaga S, Takashima E, Tsuboi T, Kohyama M, Suenaga T, Colonna M, Takagi J, Lavstsen T, Horii T, Arase H. (2017) Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors. Nature 552(7683): 101-105.
182) Koelblen T, Bergé C, Cherrier MV, Brillet K, Jimenez-Soto L, Ballut L, Takagi J, Montserret R, Rousselle P, Fischer W, Haas R, Fronzes R, Terradot L. (2017) Molecular dissection of protein-protein interactions between integrin α5β1 and the Helicobacter pylori Cag type IV secretion system. FEBS J. 284 (23): 4143-4157.
181) Hosen N, Matsunaga Y, Hasegawa K, Matsuno H, Nakamura Y, Makita M, Watanabe K, Yoshida M, Satoh K, Morimoto S, Fujiki F, Nakajima H, Nakata J, Nishida S, Tsuboi A, Oka Y, Manabe M, Ichihara H, Aoyama Y, Mugitani A, Nakao T, Hino M, Uchibori R, Ozawa K, Baba Y, Terakura S, Wada N, Morii E, Nishimura J, Takeda K, Oji Y, Sugiyama H, Takagi J, and Kumanogoh A. (2017) The activated conformation of integrin β7 as a target for multiple myeloma–specific chimeric antigen receptor T-cell therapy. Nature Med. 23(12): 1436-1443.
180) Arimori, T, Kitago, Y, Umitsu, M, Fujii, Y, Asaki, R, Tamura-Kawakami, K, and Takagi, J. (2017) Fv-clasp: An artificially designed small antibody fragment with improved production compatibility, stability, and crystallizability. Structure 25: 1611-1622.
179) Takizawa, M, Arimori, T, Taniguchi, Y, Kitago, Y, Yamashita, E, Takagi, J, and Sekiguchi, K. (2017) Mechanistic basis for the recognition of laminin-511 by α6β1 integrin. Science Advances 3(9):e1701497.
178) 的場京子、高木淳一 (2017) 糖鎖によってゆらぐ受容体蛋白質の構造を観る、医学のあゆみ、262(5), 424-430.
177) Meng H, Yamashita C, Shiba-Fukushima K, Inoshita T, Funayama M, Sato S, Hatta T, Natsume T, Umitsu M, Takagi J, Imai Y, Hattori N. (2017) Loss of Parkinson’s disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c. Nature Commun. 8: 15500.
176) Nuemket N, Yasui N, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko M.K, Takagi J, Hosotani M, Yamashita A. (2017) Structural basis for perception of diverse chemical substances by T1r taste receptors. Nature Commun. 8: 15530.
175) Hirai H, Yasui N, Yamashita K, Tabata S, Yamamoto M, Takagi J, Nogi T. (2017) Structural basis for ligand capture and release by the endocytic receptor ApoER2. EMBO Rep. pii: e201643521.
174) Shirakabe K, Omura T, Shibagaki Y, Mihara E, Homma K, Kato Y, Yoshimura A, Murakami Y, Takagi J, Hattori S, Ogawa Y. (2017) Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation. Scientific Rep. 7:46174.
173) Fukuda S, Kita S, Obata Y, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nishizawa H, Funahashi T, Takagi J, Maeda N, and Shimomura I. (2017) The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2. J Biol Chem. 292 (19), 7840-7849.
172) Matsumoto A, Miyazaki N, Takagi J, and Iwasaki K. (2017) 2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching. Scientific Rep. 7(1):377.
171) Matoba K, Mihara E, Tamura-Kawakami K, Miyazaki N, Maeda S, Hirai H, Thompson S, Iwasaki K, and Takagi J. (2017) Conformational freedom of the LRP6 ectodomain is regulated by N-glycosylation and the binding of the Wnt antagonist Dkk1. Cell Reports 18, 32-40.
170) Matsunaga Y, Bashiruddin NK, Kitago Y, Takagi J, and Suga H. (2016) Allosteric inhibition of a semaphorin 4D receptor plexin B1 by a high-affinity macrocyclic peptide. Cell Chem. Biol. 23, 1341-1350.
169) Ushioda R, Miyamoto A, Inoue M, Watanabe S, Okumura M, Maegawa K, Uegaki K, Fujii S, Fukuda Y, Umitsu M, Takagi J, Inaba K, Mikoshiba K, and Nagata K. (2016) Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by ERdj5. Proc. Natl. Acad. Sci. USA, 113, E6055-E6063.
168)高木淳一 (2016) PAタグ：ループ挿入可能な超高親和性ペプチドタグ生物物理 56（5），284-287.
167) Umitsu M, Sakai K, Ogasawara S, Kaneko M, Asaki R, Tamura-Kawakami K, Kato Y, Matsumoto K, and Takagi J. (2016) Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies. Scientific Rep. 9 (6), 33149.
166) Kitago Y, Kaneko MK, Ogasawara S, Kato Y, and Takagi J. (2016) Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1. Biochem Biophys Res Commun. 478(3):1274-9.
165) Ozawa A, Sato Y, Imabayashi T, Uemura T, Takagi J, and Sekiguchi K. (2016) Molecular Basis of the Ligand Binding Specificity of αvβ8 Integrin. J Biol Chem. 291(22):11551-65.
164) Suzuki K, Tsunoda H, Omiya R, Matoba K, Baba T, Suzuki S, Segawa H, Kumanogoh A, Iwasaki K, Hattori K, Takagi J. (2016) Structure of the plexin ectodomain bound by semaphorin-mimicking antibodies. PLoS One, 11 (6): e0156719.
163) 海津正賢、高木淳一 (2016) HGF-Metシグナル伝達の構造的基盤、日本血栓止血学会誌、27(1), 77-84.
162) Fujii Y, Matsunaga Y, Arimori T, Kitago Y, Ogasawara S, Kaneko M, Kato Y, and Takagi J. (2016) Tailored placement of a turn-forming PA tag into the structured domain of a protein to probe its conformational state. J. Cell Science, 129:1512-1522.
161) Mihara E, Hirai H, Yamamoto H, Tamura-Kawakami K, Matano M, Kikuchi A, Sato T, and Takagi J. (2016) Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin. eLife;5:e11621.
160) Morita J, Kano K, Kato K, Takita H, Skagami H, Yamamoto Y, Mihara E, Ueda H, Sato T, Tokuyama H, Arai H, Asou H, Takagi J, Ishitani R, Nishimasu H, Nureki O, and Aoki J. (2016) Structural and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase. Scientific Rep. 6, 20995.
159) Kharitidi D, Apaja PM, Manteghi S, Suzuki K, Malitskaya E, Roldan A, Gingras MC, Takagi J, Lukacs GL, Pause A. (2015) Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration. Cell Rep. 13(3):599-609.
158) 北郷　悠、高木淳一 (2015) 巨大受容体sorLAによるアミロイドβ分解の促進、医学のあゆみ、252(9), 963-964.
157) 海津正賢、高木淳一 (2015) 構造生物学が明らかにする細胞膜を介したシグナリング機構、実験医学、33(10), 183-189.
156) Kitago Y, Nagae M, Nakata Z, Yagi-Utsumi M, Takagi-Niidome S, Mihara E, Nogi T, Kato K, Takagi J. (2015) Structural basis for amyloidogenic peptide recognition by sorLA. Nature Struct. Mol. Biol. 22, 199-206.
155) Takagi, J. and Tate, CG. (2014) Editorial overview: New constructs and expression of proteins: Making things better. Curr Opin Struct Biol. 26.
154) Morita J, Kato K, Mihara E, Ishitani R, Takagi J, Nishimasu H, Aoki J, and Nureki O. (2014) Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6. Acta Crystallogr. F Struct. Biol. Commun. 70, 794-799.
153) Fujii, Y., Kaneko, M., Neyazaki, M., Nogi, T., Kato, Y. and Takagi, J. (2014) PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin. Protein Exp. Purif. 95, 240-247.
152) Caglayan S, Takagi-Niidome S, Liao F, Carlo A-S, Schmidt V, Burgert T, Kitago Y, Füchtbauer E-M, Füchtbauer A, Holtzman DM, Takagi J, and Wilnow TE. (2014) Lysosomal sorting of amyloid-β by the SORLA receptor is impaired by a familial Alzheimer’s disease mutation. Science Transl. Med., 6, 223ra20.
151) Hizukuri Y, T, Tabata S, Tamura-Kawakami K, Oda T, Sato M, Takagi J, Akiyama Y, and Nogi T. (2014) A structure-based model of substrate discrimination by a non-canonical PDZ tandem in the intramembrane-cleaving protease RseP. Structure, 22, 326-336.
150) 禾　晃和、三原恵美子、高木淳一 (2013) 動物細胞発現系を用いた試料調製、「タンパク質結晶の最前線」（杉山　成　監修）シーエムシー出版,
149) 高木淳一 (2013) 動物細胞を用いる膜タンパク質生産、「膜タンパク質構造研究」（岩田　想　編）化学同人, pp 103-110.
148) 高木淳一 (2013) 相関構造解析法による階層をまたがった生命機能の統合的解明、パリティ、28-07, 20-24.
147) 宮崎直幸、田中宏樹、禾　晃和、岩崎憲治、高木淳一 (2013) 光—電子相関顕微鏡法〜シナプス接着メカニズム解明への応用〜、生物物理、53, 218-221.
146) Akiyama M, Nakayama D, Takeda S, Kokame K, Takagi J, Miyata T. (2013) Crystal structure and enzymatic activity of an ADAMTS13 mutant with the East Asian-specific P475S polymorphism. J Thromb Haemost. 11, 1399-1406.
145) Sangawa T, Tabata S, Suzuki K, Saheki Y, Tanaka K, and Takagi J. (2013) A multi-purpose fusion tag derived from an unstructured and hyper-acidic region of the amyloid precursor protein. Protein Sci., 22., 840-850.
144) Nojima S, Toyofuku T, Kamao H, Ishigami C, Kaneko J, Okuno T, Takamatsu H, Ito D, Kang S, Kimura T, Yoshida Y, Morimoto K, Maeda Y, Ogata A, Ikawa M, Morii E, Aozasa K, Takagi J, Takahashi M, Kumanogoh A. (2013) A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration. Nature Commun. 4:1406.
143) 松永幸子、高木淳一 (2013) セマフォリン関連分子群の立体構造、実験医学、31(4), 523-530.
142) 北郷　悠、高木淳一 (2012) LDL受容体ファミリーの構造と蛋白質間相互作用、The Lipid、23(4), 56-62.
141) Kato K, Nishimasu H, Okudaira S, Mihara E, Ishitani R, Takagi J, Aoki J, Nureki O. (2012) Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling. Proc. Natl. Acad. Sci. USA, 109, 16876-16881.
140) McKee, K.K., Yang, D.H., Patel, R., Chen, Z.L., Strickland, S., Takagi, J., Sekiguchi, K., and Yurchenco, P.D. (2012) Schwann Cell Myelination Requires Integration of Laminin Activities. J. Cell Sci., 125, 4609-4619.
139) Tanaka, H., Miyazaki, N., Matoba, K., Nogi, T., Iwasaki, K., and Takagi, J. (2012) Higher-order architecture of cell adhesion mediated by polymorphic synaptic adhesion molecules neurexin and neuroligin. Cell Reports, 2(1), 101-110.
138) Kato K, Nishimasu H, Mihara E, Ishitani R, Takagi J, Aoki J, and Nureki O. (2012) Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1. Acta Crystallogr. F, 68(7), 778-782.
137) Matoba K, Takagi J, Yasunaga T, Jinnai H, Iwasaki K. (2012) Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer. J Electron Microsc (Tokyo). 61(3), 193-198.
136) Nagae, M., Re, S., Mihara, E., Nogi, T., Sugita, Y., and Takagi, J. (2012) Crystal structure of α5β1 integrin ectodomain: Atomic details of the fibronectin receptor. J. Cell Biol. 197(1), 131-140.
135) Hayashi, I., Takatori, S., Urano, Y., Takagi, J., Sakata-Yanagimoto, M., Iwanari, H., Osawa, S., Morohashi, Y. Li, T., Wong, P.C., Chiba, S. Kodama, T., Hamakubo, T., Tomita, T. and Iwatsubo, T. (2012) Neutralization of the γ-secretase activity by monoclonal antibody against extracellular domain of nicastrin. Oncogene, 31(6), 787-798.
134) 寒川　剛、高木淳一 (2011) タンパク質間相互作用の検出−表面プラズモン共鳴法、「タンパク質実験ノート（下）」（岡田雅人、三木裕明、宮崎　香　編）羊土社, pp 132-137.
133) 禾　晃和、高木淳一(2011) 神経軸索の伸長を司るガイダンス因子セマフォリンのシグナル伝達における過渡的複合体形成、生化学、83, 938-948.
132) Yasui, N., Kitago, Y., Beppu, A., Kohno, T., Morishita, S., Gomi, H., Nagae, M., Hattori, M. and Takagi, J. (2011) Functional importance of covalent homodimer of reelin protein linked via its central region. J. Biol. Chem., 286(40), 35247-35256.
131) Tanaka, H., Nogi, T., Yasui, N., Iwasaki, K. and Takagi, J. (2011) Structural Basis for Variant-Specific Neuroligin-Binding by α-Neurexin. PLoS One, 6, e19411
130) 高木淳一 (2011) 動物細胞を用いる高難度タンパク質生産と迅速精製技術、BIO INDUSTRY、 28 (3), 7-13.
129) Nishimasu, H., Okudaira, S., Hama, K., Mihara, M., Dohmae, N., Ishitani, R., Takagi, J., Aoki, J., Nureki, O. (2011) Crystal structure of Autotaxin, a secreted lysophospholipase D that generates GPCR-signaling lipid mediator. Nature Struct. Mol. Biol. 18, 205-212.
128) Nakata, Z., Nagae, M., Yasui, N., Bujo, H., Nogi, T., and Takagi, J. (2011) Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain. Acta Crystallographica., F67, 129-132.
127) 秋山正志、武田壮一、小亀浩市、高木淳一、宮田敏行(2010) VWF切断酵素ADAMTS13のエキソサイト認識機構、生化学、82, 950-956
126) Nogi, T., Yasui, N., Mihara, E., Matsunaga, Y., Noda, M., Yamashita, N., Toyofuku, T., Goshima, Y., Kumanogoh, A, and Takagi, J. (2010) Structural basis for semaphorin signaling through the plexin receptor. Nature 467, 1123-1127.
125) Kishimoto-Okada, A., Murakami, S., Ito, Y., Horii, N., Furukawa, H., Takagi, J., Iwasaki, K. (2010) Comparison of the envelope architecture of E. coli using two methods: CEMOVIS and cryo-electron tomography. J. Electron Microsc. 59, 419-426.
124) Sasaki, T., Takagi, J., Giudici, C., Yamada, Y., Arikawa-Hirasawa, E., Deutzmann, R., Timpl, R., Sonnenberg, A., Bächinger, H.P., Tonge, D. (2010) Laminin-121-Recombinant expression and interactions with integrins. Matrix Biology 29, 484-493.
123) Tabata, S., Nampo, M., Mihara, E., Tamura-Kawakami, K., Fujii, I., and Takagi, J. (2010) A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system. J. Proteomics., 73, 1777-1785.
122) Yasui N, Nogi T, Takagi J. (2010) Structural basis for specific recognition of reelin by its receptors. Structure, 18, 320-331.
121) Yasui N, Mihara E, Nampo M, Tamura-Kawakami K, Unno H, Matsumoto K, Takagi J. (2010) Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation. J. Immunol. Methods, 352, 153-160.
120) Akiyama, M., Takeda, S., Kokame, K., Junichi, T., and Miyata, T. (2009) Structural insights into von Willebrand factor cleavage by ADAMTS13. Proc. Natl. Acad. Sci. USA, 106, 19274-19279.
119) Akiyama, M., Takeda, S., Kokame, K., Junichi, T., and Miyata, T. (2009) Production, crystallization and preliminary crystallographic analysis of the exosite-containing fragment of human von Willebrand factor-cleaving proteinase, ADAMTS13. Acta Crystallographica., F65, 739-742.
118) 高木淳一 (2009) 微量蛋白質の1段階精製を可能にするTARGETタグシステム、「融合発展する構造生物学とケミカルバイオロジーの最前線」（長野哲雄、若槻壮市、高木淳一、古谷利夫　編）共立出版, pp 1468-1475.
117) 高木淳一 (2009) タンパク質の分子間相互作用の解析、「やさしい原理からはいるタンパク質科学実験法３−タンパク質の働きを知る−分子機能と生体作用」（長谷俊治、高尾敏文、高木淳一　編）化学同人, pp 29-52.
116) 高木淳一 (2009) インテグリン、「ますます重要になる細胞周辺環境（細胞ニッチ）の最新科学技術」（田畑泰彦　編）メディカルドゥ, pp 251-256.
115) Sato, Y., Uemura, T., Morimitsu, K., Sato-Nishiuchi, R., Manabe, R., Takagi, J., Yamada, M., and Sekiguchi, K. (2009) Molecular basis of the recognition of nephronectin by integrin alpha8beta1. J. Biol. Chem. 284, 14524-14536.
114) Nagae, M., Nishikawa, K., Yasui, N., Yamasaki, M., Nogi, T., and Takagi, J. (2008) Structure of F-spondin reeler domain reveals a unique -sandwich fold with a deformable disulfide-bonded loop. Acta Crystallographica., D64, 1138-1145.
113) Nogi, T., Sangawa, T., Tabata, S., Nagae, M., Tamura-Kawakami, K., Beppu, A., Hattori, M., Yasui, N., and Takagi, J. (2008) Novel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purification. Protein Sci., 17, 2120-2126.
112) Sangawa, T., Nogi, T., and Takagi, J. (2008) A murine monoclonal antibody that binds N-terminal extracellular segment of human protease activated receptor-4. Hybridoma, 27(5), 331-335.
111) Atsushi Matsumoto, Tetsuji Kamata, Junichi Takagi, Kenji Iwasaki, and Kei Yura. (2008) Key interactions in ectodomain of integrin for global conformational change detected by elastic network normal mode analysis. Biophys. J., 95(6), 2895-2908.
110) Yamaguchi, H., Takagi, J., Miyamae, T., Yokota, S., Fujimoto, T., Nakamura, S., Ohshima, S., Naka, T., and Nagata, S. (2008) Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus. J. Leukocyte Biol., 83, 1300-1307.
109) Takagi, J. (2008). Crystal structure of reelin repeats. in Reelin Glycoprotein, biology, structure and roles in health and disease. Fetami, S.H. Ed., Springer, pp57-68.
108) Takagi, J. (2007) Structural basis for ligand recognition by integrins. Curr. Opin. Cell Biol., 19, 557-564.
107) Yasui, N., Nogi, T., Kitao, T., Nakano, Y., Hattori, M., and Takagi, J. (2007) Structure of a receptor-binding reelin fragment and mutational analysis reveal a recognition mechanism similar to endocytic receptors. Proc. Natl. Acad. Sci. USA, 104, 9988-9993.
106) Takahashi, S., Leiss, M., Moser, M., Ohashi, T., Kitao, T., Heckmann, D., Pfeifer, A., Kessler, H., Takagi, T., Erickson, H.P., and Fässler, R. (2007) The RGD motif in fibronectin is essential for mouse development but dispensable for fibronectin fibril assembly. J. Cell Biol. 178, 167-178.
105) Katayama, S., Wei, T., Omura, T., Takagi, J., and Iwasaki, K. (2007) Three-dimensional architecture of virus-packed tubule. J. Electron Microsc., 56, 77-81.
104) Suzuki K., Okuno,T., Yamamoto, M., Pasterkamp, R.J., Takegahara, N., Kitao, T., Takagi, J., Rennert , P.D., Kolodkin, A.L., Kumanogoh, A., and Kikutani, H. (2007) Semaphorin 7A initiates T cell-mediated inflammatory responses through 11 integrin. Nature 446 680-684.
103) 高木淳一 (2007) ポストゲノム時代のタンパク質科学−構造・機能・ゲノミクス−（Arthur M. Lesk原著）化学同人, pp 1-328.
102) 高木淳一 (2007) 細胞接着分子とその受容体：細胞が細胞外マトリックスを識別する仕組み、「再生医療のための細胞生物学」（関口清俊　編）コロナ社, pp 26-48.
101) Jovanovic J, Takagi J, Choulier L, Abrescia NG, Stuart DI, van der Merwe PA, Mardon HJ, and Handford PA (2007) alpha Vbeta 6 is a novel receptor for human fibrillin-1: comparative studies of molecular determinants underlying integrin-RGD affinity and specificity. J. Biol. Chem. 282, 6743-6751.
100) 高木淳一 (2006) モジュラータンパク質の構造解析：受容体構造生物学における中心課題、日本結晶学会誌、48, 411-417.
99) Nogi, T., Yasui, N., Hattori, M., Iwasaki, K., and Takagi, J. (2006). Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography. EMBO J., 25, 3675–3683.
98) Tarui, T., Akakura, N., Majumdar, M., Andronicos, N., Takagi, J., Mazar, A.P., Bdeir, K., Kuo, A., Yarovoi, S.V., Cines, D.B., and Takada, Y. (2006). Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation. Thromb Haemost., 95, 524-534.
97) Nishiuchi, R., Takagi, J., Hayashi, M., Ido, H., Yagi, Y., Sanzen, N., Tsuji, T., Yamada, M., and Sekiguchi, K. (2006). Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins. Matrix Biol., 25,189-197.
96) 高木淳一 (2005) 血小板GPIIb/IIIa複合体の立体構造、血液疾患-state of the arts-、pp.116-119.
95) 高木淳一 (2005) レセプター膜貫通ドメインの構造とそのシグナリングにおける動的制御、蛋白質核酸酵素、50(10), 1226-1232.
94) Luo, B. H., Carman, C. V., Takagi, J., and Springer, T. A. (2005). Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering. Proc Natl Acad Sci U S A, 102, 3679-3684.
93) Nishiuchi, R., Sanzen, N., Nada, S., Sumida, Y., Wada, Y., Okada, M., Takagi, J., Hasegawa, H., and Sekiguchi, K. (2005). Potentiation of the ligand-binding activity of integrin alpha3 beta1 via association with tetraspanin CD151. Proc Natl Acad Sci U S A, 102, 1939-1944.
92) 高木淳一 (2005) 血小板インテグリンαIIbβ3の立体構造から解くリガンド認識の構造的基盤、日本血栓止血学会誌、16(1), 39-46
91) 岩崎憲治、高木淳一 (2005) 電子顕微鏡を用いた蛋白質の高次構造解析が開く世界−インテグリンレセプターの構造解析を中心として−、生化学、77, 400-410
90) Artoni, A., Li, J., Mitchell, B., Ruan, J., Takagi, J., Springer, T. A., French, D. L., and Coller, B. S. (2004). Integrin beta3 regions controlling binding of murine mAb 7E3: implications for the mechanism of integrin alphaIIbbeta3 activation. Proc Natl Acad Sci U S A, 101, 13114-13120.
89) Chen, J., Takagi, J., Xie, C., Xiao, T., Luo, B. H., and Springer, T. A. (2004). The relative influence of metal ion binding sites in the I-like domain and the interface with the hybrid domain on rolling and firm adhesion by integrin alpha4beta7. J Biol Chem, 279, 55556-55561.
88) Luo, B. H., Springer, T. A., and Takagi, J. (2004). A specific interface between integrin transmembrane helices and affinity for ligand. PLoS Biol, 2, 0776-0786.
87) Luo, B. H., Strokovich, K., Walz, T., Springer, T. A., and Takagi, J. (2004). Allosteric beta1 integrin antibodies that stabilize the low affinity state by preventing the swing-out of the hybrid domain. J Biol Chem, 279, 27466-27471.
86) Luo, B. H., Takagi, J., and Springer, T. A. (2004). Locking the beta3 integrin I-like domain into high and low affinity conformations with disulfides. J Biol Chem, 279, 10215-10221.
85) Takagi, J. (2004). Structural basis for ligand recognition by RGD (Arg-Gly-Asp)-dependent integrins. Biochem Soc Trans, 32, 403-406
84) 高木淳一、細胞接着におけるインテグリン“活性化”の正体、細胞工学、23(7), 832-837 (2004)
83) Xiao, T., Takagi, J., Coller, B. S., Wang, J. H., and Springer, T. A. (2004). Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics. Nature, 432, 59-67.
82) Yang, W., Shimaoka, M., Salas, A., Takagi, J., and Springer, T. A. (2004). Intersubunit signal transmission in integrins by a receptor-like interaction with a pull spring. Proc Natl Acad Sci U S A, 101, 2906-2911.
81) 高木淳一 (2003) ラミニン・ナイドジェン複合体の立体構造−蛋白質結合インターフェースの特異性と普遍性はいかに保たれるか?−、蛋白質核酸酵素、48(14), 1920-1927.
80) 高木淳一 (2003) インテグリンによるシグナル伝達、実験医学、21(1), 55-57.
79) Luo, B.-H., Springer, T. A., and Takagi, J. (2003). Stabilizing the open conformation of the integrin headpiece with a glycan wedge increases affinity for ligand. Proc Natl Acad Sci U S A, 100, 2403-2408.
78) Luo, B.-H., Springer, T. A., and Takagi, J. (2003). High affinity ligand binding by integrins does not involve head separation. J Biol Chem, 278, 17185-17189.
77) Shimaoka, M., Xiao, T., Liu, J.-H., Yang, Y., Dong, Y., Jun, C.-D., McCormack, A., Zhang, R., Joachimiak, A., Takagi, J., et al. (2003). Structures of the aL I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation. Cell, 112, 99-111.
76) 高木淳一 (2003) シグナル伝達におけるインテグリンの構造変化、日本血栓止血学会誌、14(1), 3-10.
75) Takagi, J., Strokovich, K., Springer, T. A., and Walz, T. (2003). Structure of integrin alpha5beta1 in complex with fibronectin. EMBO J, 22, 4607-4615.
74) Takagi, J., Yang, Y., Liu, J. H., Wang, J. H., and Springer, T. A. (2003). Complex between nidogen and laminin fragments reveals a paradigmatic beta-propeller interface. Nature, 424, 969-974.
73) Beglova, N., Blacklow, S. C., Takagi, J., and Springer, T. A. (2002). Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation. Nat Struct Biol, 9, 282-287.
72) Jing, H., Takagi, J., Liu, J.-h., Lindgren, S., Zhang, R.-G., Joachimiak, A., Wang, J.-h., and Springer, T. A. (2002). Archaeal surface layer proteins contain b-propeller, polycystic kidney disease, and b-helix domains, and are related to metazoan cell surface proteins. Structure, 10, 1453-1464.
71) Kamiya, S., Kato, R., Wakabayashi, M., Tohyama, T., Enami, I., Ueki, M., Yajima, H., Ishii, T., Nakamura, H., Katayama, T., et al. (2002). Fibronectin peptides derived from two distinct regions stimulate adipocyte differentiation by preventing fibronectin matrix assembly. Biochemistry, 41, 3270-3277.
70) Kohli, R. M., Takagi, J., and Walsh, C. T. (2002). The thioesterase domain from a nonribosomal peptide synthetase as a cyclization catalyst for integrin binding peptides. Proc Natl Acad Sci U S A, 99, 1247-1252.
69) 高木淳一 (2002) インテグリンαVβ3の立体構造−気まぐれな巨大細胞接着レセプターの脚線美は本物か？−、蛋白質核酸酵素 47: 153-159.
68) Shimaoka, M., Takagi, J., and Springer, T. A. (2002). Conformational regulation of integrin structure and function. Annu Rev Biophys Biomol Struct, 31, 485-516.
67) Shimaoka, M., Lu, C., Salas, A., Xiao, T., Takagi, J., and Springer, T. A. (2002). Stabilizing the integrin aM inserted domain in alternative conformations with a range of engineered disulfide bonds. Proc Natl Acad Sci U S A, 99, 16737-16741.
66) Takagi, J., Debottis, D. P., Erickson, H. P., and Springer, T. A. (2002). The role of specificity-determining loop of the integrin b-subunit I-like domain in folding, association with the a subunit, and ligand binding. Biochemistry, 41, 4339-4347.
65) Takagi, J., Petre, B. M., Walz, T., and Springer, T. A. (2002). Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Cell, 110, 599-611.
64) Takagi, J., and Springer, T. A. (2002). Integrin activation and structural rearrangement. Immunological Rev, 186, 141-163.
63) Jeon, H., Meng, W., Takagi, J., Eck, M. J., Springer, T. A., and Blacklow, S. C. (2001). Implications for familial hypercholesterolemia from structure of the LDL receptor YWTD-EGF domain pair. Nat Struc Biol, 8, 499-504.
62) Jun, C.-D., Shimaoka, M., Carman, C. V., Takagi, J., and Springer, T. A. (2001). Dimerization and the effectiveness of ICAM-1 in mediating LFA-1 dependent adhesion. Proc Natl Acad Sci U S A, 98, 6830-6835.
61) Lu, C., Shimaoka, M., Ferzly, M., Oxvig, C., Takagi, J., and Springer, T. A. (2001). An isolated, surface-expressed I domain of the integrin aLb2 is sufficient for strong adhesive function when locked in the open conformation with a disulfide. Proc Natl Acad Sci U S A, 98, 2387-2392.
60) Lu, C., Shimaoka, M., Zang, Q., Takagi, J., and Springer, T. A. (2001). Locking in alternate conformations of the integrin aLb2 I domain with disulfide bonds reveals functional relationships among integrin domains. Proc Natl Acad Sci U S A, 98, 2393-2398.
59) Lu, C., Ferzly, M., Takagi, J., and Springer, T. A. (2001). Epitope mapping of antibodies to the C-terminal region of the integrin b2 subunit reveals regions that become exposed upon receptor activation. J Immunol, 166, 5629-5637.
58) Lu, C., Takagi, J., and Springer, T. A. (2001). Association of the membrane-proximal regions of the a and b subunit cytoplasmic domains constrains an integrin in the inactive state. J Biol Chem, 276, 14642-14648.
57) Shimaoka, M., Lu, C., Palframan, R., von Andrian, U. H., Takagi, J., and Springer, T. A. (2001). Reversibly locking a protein fold in an active conformation with a disulfide bond: integrin aL I domains with high affinity and antagonist activity in vivo. Proc Natl Acad Sci U S A, 98, 6009-6014.
56) Takagi, J., Erickson, H. P., and Springer, T. A. (2001). C-terminal opening mimics "inside-out" activation of integrin a5b1. Nature Struct Biol, 8, 412-416.
55) Takagi, J., Beglova, N., Yalamanchili, P., Blacklow, S. C., and Springer, T. A. (2001). Definition of EGF-like, closely interacting modules that bear activation epitopes in integrin b subunits. Proc Natl Acad Sci U S A, 98, 11175-11180.
54) Huang, C., Zang, Q., Takagi, J., and Springer, T. A. (2000). Structural and functional studies with antibodies to the integrin b2 subunit: a model for the I-like domain. J Biol Chem, 275, 21514-21524.
53) Shimaoka, M., Shifman, J. M., Jing, H., Takagi, J., Mayo, S. L., and Springer, T. A. (2000). Computational design of an integrin I domain stabilized in the open, high affinity conformation. Nature Struct Biol, 7, 674-678.
52) Springer, T. A., Jing, H., and Takagi, J. (2000). A novel Ca2+-binding b-hairpin loop better resembles integrin sequence motifs than the EF-hand. Cell, 102, 275-277.
51) Takahashi, H., Isobe, T., Horibe, S., Takagi, J., Yokosaki, Y., Sheppard, D., and Saito, Y. (2000). Tissue transglutaminase, coagulation factor XIII, and the pro- polypeptide of von Willebrand factor are all ligands for the integrins alpha 9beta 1 and alpha 4beta 1. J Biol Chem, 275, 23589-23595.
50) Zang, Q., Lu, C., Huang, C., Takagi, J., and Springer, T. A. (2000). The top of the I-like domain of the integrin LFA-1 b subunit contacts the a subunit b-propeller domain near b-sheet 3. J Biol Chem, 275, 22202-22212.
49) Isobe, T., Takahashi, H., Ueki, S., Takagi, J., and Saito, Y. (1999). Activity-independent cell adhesion to tissue-type transglutaminase is mediated by alpha4beta1 integrin. Eur J Cell Biol, 78, 876-883.
48) Ueki, S., Takagi, J., Kobayashi, Y., Sato, F., and Saito, Y. (1999). 12-hydroxy-5Z, 8Z, 10E, 14Z, eicosatetraenoic acid (12-HETE) stimulates cAMP production in normal human fibroblasts. J Cell Physiol, 178, 63-68.
47) Wakasugi, M., Kawachi, H., Omori, S., Takagi, J., Nishi, S., Arakawa, M., and Shimizu, F. (1999). Expression of the molecule detected by anti-propolypeptide of von Willebrand factor antibody in rat mesangial cells in anti-Thy-1.1 mAb 1-22-3-induced glomerulonephritis. -A marker of injured mesangial cells-. Nephron,
46) Garcia, A. J., Takagi, J., and Boettiger, D. (1998). Two-stage activation for alpha5beta1 integrin binding to surface- adsorbed fibronectin. J Biol Chem, 273, 34710-34715.
45) Kushida, A., Koga, M., Takagi, J., and Saito, Y. (1998). Screening of hybridoma clones producing antibodies against plasma membrane-associated materials. Hybridoma, 17, 209-213.
44) Takagi, J. (1998). Molecular view of the integrin cell adhesion receptors. Connective Tissue, 30,
43) 高木淳一：細胞の細胞外マトリックスへの接着機構の解析法、「細胞外マトリックス研究法」（畑　隆一郎、服部俊治、新井克彦　編） pp. 9-15, コラーゲン技術研修会(1998)
42) Tsuchida, J., Ueki, S., Takada, Y., Saito, Y., and Takagi, J. (1998). The 'ligand-induced conformational change' of alpha 5 beta 1 integrin. Relocation of alpha 5 subunit to uncover the beta 1 stalk region. J Cell Sci, 111, 1759-1766.
41) Inuyama, H., Saito, T., Takagi, J., and Saito, Y. (1997). Factor X-dependent, thrombin-generating activities on a neuroblastoma cell and their disappearance upon differentiation. J Cell Physiol, 173, 406-414.
40) Isobe, T., Hisaoka, T., Shimizu, A., Okuno, M., Aimoto, S., Takada, Y., Saito, Y., and Takagi, J. (1997). Propolypeptide of von Willebrand factor is a novel ligand for VLA-4 integrin. J Biol Chem, 272, 8447-8453.
39) Takagi, J., Kamata, T., Meredith, J., Puzon-McLaughlin, W., and Takada, Y. (1997). Changing ligand specificities of alphavbeta1 and alphavbeta3 integrins by swapping a short diverse sequence of the beta subunit. J Biol Chem, 272, 19794-19800.
38) Takagi, J., Isobe, T., Takada, Y., and Saito, Y. (1997). Structural interlock between ligand-binding site and stalk-like region of beta1 integrin revealed by a monoclonal antibody recognizing conformation-dependent epitope. J Biochem, 121, 914-921.
37) Tsuchida, J., Ueki, S., Saito, Y., and Takagi, J. (1997). Classification of 'activation' antibodies against integrin beta1 chain. FEBS Lett, 416, 212-216.
36) Ueki, S., Takagi, J., and Saito, Y. (1996). Dual fanctions of transglutaminase in novel cell adhesion. J Cell Science, 109, 2727-2735.
35) Wang, X.-Q., Otsuka, M., Takagi, J., Kobayashi, Y., Sato, F., and Saito, Y. (1996). Inhibition of adenylyl cyclase by 12(S)-hydroxyeicosatetraenoic acid. Biocem Biophys Res Commun, 228, 81-87.
34) Kondoh, O., Muto, A., Kajiwara, S., Takagi, J., Saito, Y., and Shishido, K. (1995). A fruiting body-specific cDNA, mfbAc, from the mushroom Lentinus edodes encodes a high-molecular-weight cell-adhesion protein containing an Arg-Gly-Asp motif. Gene, 154, 31-37.
33) Takagi, J., and Saito, Y. (1995). Differential role of protein tyrosine phosphorylation/ dephospho- rylation in affinity modulation of beta1 and beta3 integrin in human fibroblasts. Cell Struct Funct, 20, 403-413.
32) 高木淳一：インテグリンスーパーファミリー、生体の科学 46: 530-532 (1995)
31) Takagi, J., Aoyama, T., Ueki, S., Ohba, H., Saito, Y., and Lorand, L. (1995). Identification of factor-XIIIa-reactive glutaminyl residues in the propolypeptide of bovine von Willebrand factor. Eur J Biochem, 232, 773-777.
30) Takagi, J., Sudo, Y., Saito, T., and Saito, Y. (1994). Beta 1-integrin-mediated adhesion of melanoma cells to the propolypeptide of von Willebrand factor. Eur J Biochem, 222, 861-867.
29) Takagi, J., Fujisawa, T., Usui, T., Aoyama, T., and Saito, Y. (1993). A single chain 19-kDa fragment from bovine thrombospondin binds to type V collagen and heparin. J Biol Chem, 268, 15544-15549.
28) Usui, T., Takagi, J., and Saito, Y. (1993). Propolypeptide of von Willebrand factor serves as a substrate for factor XIIIa and is cross-linked to laminin. J Biol Chem, 268, 12311-12316.
27) 高木淳一、関屋富士男、斉藤佑尚：血小板トロンビンレセプター、血液・腫瘍科　 27: 437-449 (1993)
26) Hashimoto, K., Sekiya, F., Takagi, J., Tsukada, T., Sato, F., and Saito, Y. (1992). Activation of phospholipases in platelets by polyclonal antibodies against a surface membrane protein. Biochim Biophys Acta, 1165, 27-31.
25) Takagi, J., Asai, H., and Saito, Y. (1992). A collagen/gelatin-binding decapeptide derived from bovine propolypeptide of von Willebrand factor. Biochemistry, 31, 8530-8534.
24) Usui, T., Fujisawa, T., Takagi, J., and Saito, Y. (1992). Propolypeptide and mature portions of von Willebrand factor of bovine origin recognize different sites on type-I collagen obtained from bovine tendon. Eur J Biochem, 205, 363-367.
23) Fujisawa, T., Takagi, J., Sekiya, F., Goto, A., Miake, F., and Saito, Y. (1991). Monoclonal antibodies that inhibit binding of propolypeptide of von Willebrand factor to collagen. Localization of epitopes. Eur J Biochem, 196, 673-677.
22) Hashimoto, K., Usui, T., Sasaki, K., Fujisawa, T., Sekiya, F., Takagi, J., Tsukada, T., and Saito, Y. (1991). Immunological detection of propolypeptide of von Willebrand factor on platelet surface. Biochem Biophys Res Commun, 176, 1571-1576.
21) 高木淳一、関屋富士男、斉藤佑尚：“コラーゲンによる血小板凝集”の選択的阻害、血液・腫瘍科 23: 391-398 (1991)
20) 高木淳一、関屋富士男、藤沢朋行、橋本恵子、碓氷智子、斉藤佑尚：コラーゲンによる血小板の活性化、「血小板活性化の生化学」（日高、山崎、大熊、青木　編） pp. 189-204, 金芳堂 (1991)
19) Sekiya, F., Takagi, J., Usui, T., Kawajiri, K., Kobayashi, Y., Sato, F., and Saito, Y. (1991). 12S-hydroxyeicosatetraenoic acid plays a central role in the regulation of platelet activation. Biochem Biophys Res Commun, 179, 345-351.
18) Takagi, J., Fujisawa, T., Sekiya, F., and Saito, Y. (1991). Collagen-binding domain within bovine propolypeptide of von Willebrand factor. J Biol Chem, 266, 5575-5579.
17) Sekiya, F., Kawajiri, K., Takagi, J., Saito, Y., and Nagase, S. (1990). Albumin in plasma potentiates platelet aggregation induced by collagen--a study with an albumin deficient rat. Thromb Res, 58, 657-662.
16) Sekiya, F., Takagi, J., Sasaki, K., Kawajiri, K., Kobayashi, Y., Sato, F., and Saito, Y. (1990). Feedback regulation of platelet function by 12S-hydroxyeicosatetraenoic acid: inhibition of arachidonic acid liberation from phospholipids. Biochim Biophys Acta, 1044, 165-168.
15) Sekiya, F., Takagi, J., and Saito, Y. (1989). Elucidation of a role of plasma albumin during collagen-induced aggregation of bovine platelets. Thromb Res, 56, 407-415.
14) Takagi, J., Kasahara, K., Sekiya, F., Inada, Y., and Saito, Y. (1989). A collagen-binding glycoprotein from bovine platelets is identical to propolypeptide of von Willebrand factor. J Biol Chem, 264, 10425-10430.
13) Takagi, J., Sekiya, F., Kasahara, K., Inada, Y., and Saito, Y. (1989). Inhibition of platelet-collagen interaction by propolypeptide of von Willebrand factor. J Biol Chem, 264, 6017-6020.
12) Kasahara, K., Takagi, J., Sekiya, F., Inada, Y., and Saito, Y. (1988). "A" subunit of factor XIII is present on bovine platelet membrane and mediates collagen-induced platelet activation. Thromb Res, 50, 253-263.
11) Sekiya, F., Takagi, J., Kasahara, K., Inada, Y., and Saito, Y. (1988). Plasma albumin is essential for collagen-induced platelet aggregation. Thromb Res, 50, 837-846.
10) Takagi, J., Kasahara, K., Sekiya, F., Inada, Y., and Saito, Y. (1988). Subunit B of factor XIII is present in bovine platelets. Thromb Res, 50, 767-774.
9) Takagi, J., Sekiya, F., Kasahara, K., Inada, Y., and Saito, Y. (1988). Venom from southern copperhead snake (Agkistrodon contortrix contortrix). II. A unique phospholipase A2 that induces platelet aggregation. Toxicon, 26, 199-206.
8) 高木淳一、斉藤佑尚、稲田祐二：血小板中の血液凝固XIII因子はコラーゲンレセプターか？　生化学、59: 13-17 (1987)
7) Kasahara, K., Takagi, J., Sekiya, F., Inada, Y., and Saito, Y. (1987). Analysis of distribution of receptors among platelets by flow cytometry. Thromb Res, 45, 763-770.
6) Shimizu, A., Jimenez, E. C., Takagi, J., Inada, Y., and Saito, Y. (1987). Venom from southern copperhead snake (Agkistrodon contortrix contortrix). I. Characterization of a protease that preferentially releases fibrinopeptide B. Toxicon, 25, 751-757.
5) Imada, T., Saito, Y., Shimada, H., Kikuchi, T., Takagi, J., and Inada, Y. (1986). Specific desensitization in the cytoskeleton assembly of platelet by receptor dependent activations. Thromb Res, 41, 119-125.
4) Kikuchi, T., Takagi, J., Inada, Y., and Saito, Y. (1986). Interaction between plasma Factor XIII and collagen. Thromb Res, 43, 213-218.
3) Saito, Y., Imada, T., Takagi, J., Kikuchi, T., and Inada, Y. (1986). Platelet Factor XIII; The collagen receptor? J Biol Chem, 261, 1355-1358.
2) Takagi, J., Imada, T., Saito, Y., Kikuchi, T., and Inada, Y. (1986). Modification of transglutaminase assay: Use of ammonium sulfate to stop the reaction. Anal Biochem, 153, 295-298.
1) Takagi, J., Imada, T., Kikuchi, T., Saito, Y., and Inada, Y. (1985). A new platelet aggregation factor from "Gynostemma pentaphyllum Makino". Chem Pharm Bull, 33, 5568-5571.

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